Báo cáo khoa học: Enhanced stereoselective hydrolysis of toxic organophosphates by directly evolved variants of mammalian serum paraoxonase ppt

... al. Enhanced stereoselective OP hydrolysis by PON1 Enhanced stereoselective hydrolysis of toxic organophosphates by directly evolved variants of mammalian serum paraoxonase Gabriel Amitai 1 , ... faster hydrolysis of the less toxic optical isomer of cyclosarin [P(+)] and the two less toxic diastereoisomers of soman [P(+)C(+ ⁄ –)] by V346A. St...

Ngày tải lên: 16/03/2014, 13:20

... and, in terms of execution speed, may 4 Acknowledgements: This corpus was provided by Dr. Daisuke Kawahara of NICT. 5 To be precise, we need two copies of these. 6 Each node has a copy of the training ... “type”, or it ended with “name” followed by of . This rule is for treating expressions like “ is one of the landlocked countries.” By applying this method of extraction to...

Ngày tải lên: 20/02/2014, 09:20

... energies of the G194P were lower than those of the WT enzyme (Table 2). As the structure of the G194P mutant become more stable than that of the WT enzyme, the rmsf values (calculated by the MDS) of ... 1, whereas the T 50 of G198P was lower than that of the WT enzyme. The T 50 of the double point mutant (G194P ⁄ G198P) was similar to that of the WT enzyme and between thos...

Ngày tải lên: 15/02/2014, 01:20

... net increase of 0.0819 of correct boundaries which led to the in- creased recall. Since the deduction of precision is less than the increase of recall, a better over-all performance of PROMODES-H ... data by expectation maximization 2 which makes them either supervised or unsupervised algorithms. The purpose of this paper is an analysis of the underlying probabilistic models a...

Ngày tải lên: 20/02/2014, 04:20

... of the targets of regulation by the kinase [3]. Recently it has been shown that deregulation of Cdc6, ORC (origin recognition com- plex) and Mcm, all of which are targets of phosphorylation by ... expression of Mcm genes in growth-arrested cells is induced by addition of serum [43]. There are several E2F binding sites in the promoter region of the Mcm5, 6,and7 genes, a...

Ngày tải lên: 20/02/2014, 23:20

... yeast. Saccharomyces cerevisiae is often used for industrial production of recombinant p eptides [1]. Secretion of heterologously expressed proteins i s obtained by expression of fusion proteins in which ... the amount of proCCK that enters the secretory pathway. Gene disruption of CYM1 thereby resulted in an increase in t he amount of secr eted proCCK. Interestingly, gene disru...

Ngày tải lên: 07/03/2014, 16:20

... 30% of the X 6 was hydrolyzed. After 60 min of incubation, the reaction was terminated. For XBS samples, this was carried out by the addition of 20 lL of NaOH (2.00 m) and subsequent boiling of ... determine the solubilization of the sub- strates by XBS, XAN and its variants, 20 mg of substrate was incubated with enzyme in a final volume of 7.80 mL after 10 min of pre-in...

Ngày tải lên: 14/02/2014, 19:20

... [12]. Considering that dmrt 1bY has all the features of the master regula- tor of testicular differentiation in medaka (see [13] for review) and because of the discovery of sex-chromo- some-linked ... network of dmrt1 and cyp19a1 in gonadal development. Expression, regulation and functions of dmrt1a/dmrt 1bY in medaka In the medaka, which has XY–XX sex determination, dmrt 1bY, t...

Ngày tải lên: 14/02/2014, 19:20

... induces the expression of Hsp70 in mammalian cells. Here, we examined the mechanism by which Hsp105b induces the expres- sion of Hsp70. Using a series of deletion mutants of Hsp105b, it was revealed ... nucleus. Downregulation of STAT3 expression resulted in reduction of the activation of the hsp70 promoter by Hsp105b. Furthermore, downre- gulation of Hsp105b reduced the...

Ngày tải lên: 18/02/2014, 06:20

... killing, the scavenging of free radicals and the reactivation of degenerated proteins during infection by a family of proteins named ‘thio- redoxins’ is one of the elegant mechanisms of defense and self-sustenance ... the N-terminus of a-2 helix of thioredoxin fold and is separated by a kink from rest of the helix due to presence of a proline [25,48–50]. The kink provide...

Ngày tải lên: 18/02/2014, 12:20