Báo cáo khoa học: Binding and activation of nitric oxide synthase isozymes by calmodulin EF hand pairs potx

... iso- forms of NOS with CaM-TnC chimeras demonstrated that the roles of the four EF hands in the binding and activation of the cNOS and iNOS enzymes are distinct [13]. Replacement of any CaM EF hand by ... two repeats of the N-terminal EF hand pair (residues 1–81, 9–75), and CaMCC contains two repeats of the C-terminal EF hand pair (residues 1–8, 82–148, 7...

Ngày tải lên: 16/03/2014, 13:20

... the presence of cytokines. Natural ligands of ICAM- 1and LFA-1 are expressed on the surface of T and B lymphocytes, natural killer cells, monocytes, macrophages and granulocytes [33], and interaction ... and macrophages. J Immunol 178, 463–473. 31 Gold R, Toyka KV & Hartung HP (1995) Synergistic effect of IFN-gamma and TNF-alpha on expression of immune molecules and...

Ngày tải lên: 18/02/2014, 18:20

... other hand, the timing of radical rearrangement (radical clocks) may depend critically on the tightness of the radical cage and the ensemble of steric and electronic forces experienced by the incipient ... ompetition with consumma- Scheme 9. Epoxidation of menadione (A) and deformylation of an enolized mimic of the A/B ring moiety of androstenedione (B) by [Fe(III)(TM...

Ngày tải lên: 19/02/2014, 16:20

... coordi- nates of the peptide bond atoms of the catalytic residues of the proteins, His50, Glu96 and His137 in a-sarcin and His36, Glu82, and His123 in a-sarcin D(7–22). Images were generated by the MOLMOL ... can be explained by the sequence variations between Aspf1 and a-sarcin, and also by the loss of a region of the protein in the deletion mutant [10,16]. Taking i...

Ngày tải lên: 16/03/2014, 19:20

... with binding of both MOF and Tip60 (equivalent to Esa1 in yeast NuA4) [124,130]. However, in human cells depletion of MOF, but not Tip60, results in reduced global levels of AcH4-K16 and defective ... acetylation and deacetyla- tion, and transient recruitment of remodellers and transcription factors. A cycle of transcription commences with the recruit- ment of transcri...

Ngày tải lên: 14/02/2014, 18:20

... by the activation of ERK1 ⁄ 2 and enhances the activity of ERK1 ⁄ 2by repressing negative regulators of the ERK ⁄ MAPK sig- naling pathway. Some other miRNAs are also reported to be induced by ... expression of estrogen receptor-a (ERa) is autoregulated by miR-18a, -19b and -20b, which in turn are upregulated by the activation of ERa. This mechanism of regulation...

Ngày tải lên: 14/02/2014, 19:20

... important mediator of the cells’ paracrine anti-fibrotic effects. These findings help to improve our understanding of the cellular and molecular basis of MSCs’ anti-inflam- matory and paracrine effects. Materials ... to treat cardiac fibroblasts, enabling observation of the paracrine effects of MSCs. The expression and secre- tion of IL-1b, TNF-a and IL-10 by hypoxia ⁄ SD-st...

Ngày tải lên: 18/02/2014, 04:20

... differently from the rest of the promoters. Of these, amino acid residues 243 and 275 are involved in DNA binding and 244 and 246 are in very close proximity to DNA binding. This indi- cates ... advantage of p53 mutation analysis, and a unique feature of this database, is the availabil- ity of the residual activity of the majority of p53 mis- sense mutants. The biolog...

Ngày tải lên: 18/02/2014, 11:20

... presented by a mixture of clo- sely related isoforms. In the present study, four pulchel- lin isoforms were isolated, and their amino acids sequences deduced by cDNA cloning and verified by MS. Half ... discussed. Results Nomenclature of the toxic pulchellin lectins The abbreviation P is followed by the Roman numer- als I, II, III and IV and refers to each pulchellin isofor...

Ngày tải lên: 18/02/2014, 16:20

... lg), pEF-BOS–p22 phox (1 lg), pEF-BOS–myc- Noxa1 (1 lg), and the indicated amount of pEF-BOS–HA-Noxo1b (left panel) or pEF-BOS–HA-Noxo1c (right panel). Superoxide pro- duction was assayed by chemiluminescence ... (wild-type), pEF-BOS–HA- Noxo1-DPX, or pEF-BOS vector in (B); pcDNA3.0–Nox3 and pEF-BOS–p22 phox , and simultaneously with pEF-BOS–HA-Noxo1b (wild-type), pEF-BOS–HA-Noxo1-D...

Ngày tải lên: 19/02/2014, 06:20