Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot

... re-replication of DNA in a single cell cycle. Inactivation of CDK1 leads to re-replication of DNA in eukaryotic cells including human cells [7]. Targets of the kinase in the regulation of DNA replication include ... HeLa cell cycle in a site-specific manner. Cyclin-dependent protein kinase is involved in the phosphorylation of MCM4 To determine whic...

Ngày tải lên: 16/03/2014, 13:20

... and the change of the molecular species composition of the cel- lular Gb 3 [28,29]. In in vitro binding assays, the fatty acid chain of Gb 3 was found to in uence the binding to Shiga toxin [30,31]. In ... transport of the holotoxin and the delivery of the monomeric catalytic A-subunit into the cytosol, leading to the inhibition of protein biosynthe- sis [...

Ngày tải lên: 30/03/2014, 10:20

... endocytosis [16]. In Madin–Darby canine kidney (MDCK) cells, a study of chimeric forms of DPP IV has shown that the luminal domain of DPP IV carries dominant apical sorting information while the short ... signal observed 15 min following insulin injection (Fig. 2A, lower panel). We then verified DPP IV phosphorylation in vitro. Following incubation of the nonsolubilized G...

Ngày tải lên: 19/02/2014, 07:20

... reveal the changes in the thermal unfolding of actin induced by interaction of G-actin with actin-binding proteins [25– 27], G–F transformation of actin, and stabilization of F-actin by phalloidin ... on the conformation of the nucleotide-binding cleft. This suggestion is sup- ported by the studies on the effects of actin-binding proteins on actin structure. Actin-bi...

Ngày tải lên: 06/03/2014, 22:21

... mixing (lane 1). At 4 h of incubation (lane 2), the bands of partially dissociated urease (a m b n ) became faint as well as the band of the b-subunit monomer. In contrast, the intensity of the ... HpU-9-L, again indicating the digestion by HpU-9-L was specific. In summary, we succeeded in the pre- paration of a catalytic antibody light chain capable of specifica...

Ngày tải lên: 07/03/2014, 21:20

... chosen for the input word in training. 8 The ATB generates normalized forms of certain clitics and of the word stem, so that the resulting tokens are not simply the result of splitting the original ... use the no- tion of ambiguity class in the feature classifiers; in- stead we investigate different ways of using the re- sults of the individual feature cla...

Ngày tải lên: 20/02/2014, 15:20

... histone loading, did not detect differences in the amount of histone H3 between the two strains, indicating that cells lacking Hat1 display normal levels of histones during the course of HU treatment. To ... treatment. To investigate whether HU induces an increase of the Hat1 protein, we used a yeast strain expressing a hemagglutinin (HA)-tagged version of Hat1. The...

Ngày tải lên: 07/03/2014, 05:20

... contained in the N-terminal part of the molecule comprising the head domain and possibly parts of the rod. The head domain of vimentin had previously been shown to harbor the binding site(s) for the ... (1995) Utrophin actin binding domain: analysis of actin binding and cellular targeting. J. Cell Sci. 108, 63–71. 10. Gimona, M. & Winder, S.J. (1998) Single calpo...

Ngày tải lên: 07/03/2014, 15:20

... deciphering the origin of the destabilization and hence, diminution of the melting temperature (T m ) in the former. Base stacking in the vicinity of A C mismatch in PNAÆDNA and DNA duplexes Intra ... to interrupted stacking, fluctuating nature of the hydro- gen bond and water organization in the vicinity of the mismatch might be the contributing factors for...

Ngày tải lên: 07/03/2014, 21:20

... that the next point is in the valid region, we project each point during the line search back onto the chosen orthant. 1 At each iteration, we choose the orthant containing the current point ... that the value of  is adap- tively chosen according to the reduction of ExpLoss during training. The algorithm starts with a large initial , and then at each forward...

Ngày tải lên: 08/03/2014, 02:21