Báo cáo khoa học: ˚ cDNA cloning and 1 75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosaminebinding hemagglutinin from Parkia platycephala seeds potx

Báo cáo khoa học: ˚ cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosaminebinding hemagglutinin from Parkia platycephala seeds potx

... The Authors Journal compilation ª 2006 FEBS cDNA cloning and 1. 75 A ˚ crystal structure determination of PPL2, an endochitinase and N-acetylglucosamine-binding hemagglutinin from Parkia platycephala ... Mimosoideae thathave been functionally and structurally characterizedare those from seeds of species of the genus Parkia, including Parkia speciosa [14 ], Parkia javanica [15 ], Parkia discolor [16 ] and ... 2 71 280. 17 Cavada BS, Santos CF, Grangeiro TB, Moreira da SilvaLIM, Campos MJO, de Sousa FAM & Calvete JJ (19 97) Isolation and partial characterization of a lectin from Parkia platycephala...

Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx

... nanoscale architectures and outstandingmechanical performance, have received a great deal of attention from many biology and materials scientistsin the past few decades [1] . Shells and pearls ... oyster CaM and CaLP. In the presence of calcium, oyster CaLP and CaM appeared as a single band with an apparentmolecular weight of approximately 18 kDa and 14 kDa, respectively, whereas in the absence ... margaritifera (Linnaeus). J Shellﬁsh Res 16 , 16 9 17 7.39 Garcia-Gasca A, Ochoa-Baze BI & Betancourt M (19 94) Microscopic anatomy of the mantle of the pearloyster Pinctada Mazatlanica (Hanley, 18 56)....

Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx

... 5¢-GACCTGCTTCCCGATGACTTT-3¢ and antisense, 5¢-TTCCTCCAACCACAGCACATAC-3¢); UBC9(sense, 5¢-CAACAAAGAACCCTGATGGCACGA-3¢ and antisense, 5¢-GCATCCGTAGCTTGAACAAGCCTC-3¢);PIAS3 (sense, 5¢-ACTGCAGGGACCCTGCTACA-3¢ and antisense, ... 5¢-GAGAATCCAGCTTCTTTCCC-3¢ and antisense, 5¢-GGCGACACTGTATGAATTGC-3¢);SENP2 (sense, 5¢-AACAGTCTCTACAATGCGGCCA-3¢ and antisense, 5¢-CCGTGTTCCATTACAAGCAGAA-3¢);SAE1 (sense, 5¢-GACCTGCTTCCCGATGACTTT-3¢ ... 5¢-CTTGATCAGTGCTCGGGAATG-3¢); PIASxa(sense, 5¢-TGCACCTCATTCACCGTCAT-3¢ and antisense,5¢-CTCAAACGTGGGCTTAGTGTCTT-3¢); PIASxb (sense,5¢-CCTTCTACTTCCATTGCACCTCAT-3¢ and anti-sense, 5¢-AAACGTGGGCTTAGTGTCTTGAA-3¢);...

Báo cáo khoa học: 2-Oxo acid dehydrogenase complexes in redox regulation Role of the lipoate residues and thioredoxin pot

... dihydro-lipoate/lipoate ratio. Redox state of the complex-boundlipoate is an indicator of the availability of the reactionsubstrates (2-oxo acid, CoA and NAD+) and thiol-disulﬁdestatus of the ... decreased number of lipoyl domainsare at a physiological disadvantage. They exhibit decreasedgrowth rates and are eventually Ôwashed-outÕ from themixed population containing the mutant and wild type ... to the state of the mitochondrial NAD+ and NADH pool. The E1activity is regulated both upon accumulation of NADH and decrease of NAD+. The E1 inactivation at low NAD+concentration prevents...

Báo cáo khoa học: "Enhancing Unlexicalized Parsing Performance using a Wide Coverage Lexicon, Fuzzy Tag-set Mapping, and EM-HMM-based Lexical Probabilities" ppt

... quantities of training data than treebanks can provide, and lexicon-based unsupervised approaches to POStagging are practically unlimited in the amount of training data they can use. POS taggers ... may be different than the goldones, and evalb can not be used. Instead, we usethe evaluation measure of (Tsarfaty, 2006), alsoused in (Goldberg and Tsarfaty, 2008), which is an adaptation of ... Lexical probabilities for rareevents are estimated in a semi-supervisedmanner from a lexicon and large unanno-tated corpora. We show that this solu-tion greatly enhances the performance of an...

Báo cáo khoa học: Kininogen-derived peptides for investigating the putative vasoactive properties of human cathepsins K and L docx

... investigating the putative vasoactiveproperties of human cathepsins K and LClaire Desmazes 1 , Laurent Galineau 1 , Francis Gauthier 1 , Dieter Bro¨ mme2 and Gilles Lalmanach 1 1Laboratoire ... increase microvascularpermeability and modulate the release of histamine,prostaglandine E2, superoxide radicals and pro-inﬂamma-tory cytokines, IL -1 and TNF -a [4–7]. Since the character-ization ... [28]. Samples: lane 1, hK1; lane 2, hK1/HMWK (molar ratio, 0 .1) ; lane 3, hK1/HMWK (molar ratio, 0. 01) ; lane 4, HMWK; lane 5,cathepsin L/HMWK (molar ratio, 2); lane 6, cathepsin L/HMWK (molar ratio,...

Báo cáo khoa học: Escherichia coli cyclophilin B binds a highly distorted form of trans-prolyl peptide isomer doc

... Suc-Ala-trans-Pro-Ala-pNA and Ac-Ala-Ala-trans-Pro-Ala-AMC (Figs 4 and 7A) , the Ng2atom of Arg48 forms a hydrogen bond withthe distance of 2.62 A ˚ and 2.72 A ˚ to CO of trans-Pro of thetripeptide and tetrapeptide, ... the Ala-cis-Pro amidebond.In the case of CyPB in complexes with Suc-Ala-trans-Pro-Ala-pNA and Ac-Ala-Ala-trans-Pro-Ala-AMC, the largedistortion in the orientation of Ala1 and Ala2 of Ala-Prowith ... distance of the N atom of Pro and the Ng2atom of Arg48 is 3.08 A ˚ and 3.02 A ˚ , and the angle between the Ng2-N vector and normal vector to the amide bond plane of Ala-Pro is 16 ° and 17 °,...

Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf

... min at 72 °Cwith a 5 min preheat at 95 °C and a 10 min ﬁnal extensionat 72 °C using primers, S1 (5¢-GCAAATGCAACTGGAAGCGG-3¢)andA1(5¢-ACAGCCTGCTAGCAAAGAGG-3¢) for ampliﬁcation of HRT1, and ... participating in natural rubber biosynthesisKasem Asawatreratanakul 1, 2, Yuan-Wei Zhang 1, *, Dhirayos Wititsuwannakul3, Rapepun Wititsuwannakul4,Seiji Takahashi 1 , Atiya Rattanapittayaporn4 and ... related to proteins from Hevea blasiliensis and Par-thenium argentatium. Phytochemistry 32, 10 97 11 02. 12 . Kang, H., Kang, M.Y. & Han, K H. (2000) Identiﬁcation of natural rubber and characterization...

Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot

... been identiﬁed and can be divided intoCHH -A and CHH-B groups [19 ]. In the crabs Can-cer pagurus, Carcinus maenas and Libinia emarginata and in the crayﬁshes Procambarus clarkii and Orconec-tes ... were allowed to anneal bymixing equal amounts of each strand, heating to 10 0 °C for 1 min, and cooling gradually to room temperature for3–4 h. Single-stranded RNAs and the annealed RNA(dsRNA) ... sequence, and 2.5 units of Taq DNA polymerase(Heber Biotec S .A. , Havana, Cuba). The ampliﬁcation of CHH cDNA was carried out in 30 cycles as follows: denatur-ation 30 s at 95 °C, 1 min annealing at...

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulﬁde isomerase family proteins with nonclassic active center motifs pdf

... translation from AUG3. For GmPDIL-3b, a 59 kDa polypeptide wasgenerated from wild-type mRNA, and from mRNAthat contained AGG in place of both AUG1 and AUG2 (Fig. 1C, lanes 2–5), whereas it was ... were treated without (lanes 1 and 2) or with (lanes 3 and 4) proteinase K, in the absence (lanes 1 and 3) or presence (lanes 2 and 4) of Triton X -10 0. Microsomalproteins (10 lg) were analyzed ... with a Thermal Cycler Dice RealTime System (TaKaRa Bio Inc.). Forward primers 5¢-CGTTTGAAGGGTGAGGAGGAAAA[FAM]G-3¢ and 5¢-CACAAGAGAGTTCTGCGATAACCTTG[FAM]G-3¢ (Invi-trogen Corporation) and reverse...

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