Báo cáo khoa học: Structural insights into mechanisms of non-nucleoside drug resistance for HIV-1 reverse transcriptases mutated at codons 101 or 138 pot

... 2006 The Authors Journal compilation ª 2006 FEBS Structural insights into mechanisms of non-nucleoside drug resistance for HIV-1 reverse transcriptases mutated at codons 101 or 138 Jingshan Ren 1 , ... priority for continued efforts to treat HIV. Experimental procedures Crystallization and data collection Expression vectors for HIV-1 RT (HXB-2 isolate...

Ngày tải lên: 16/03/2014, 13:20

... comprised denaturation at 95 °C for 60 s, annealing at 60 °C for 90 s, and extension at 72 °C for 90 s) and a final extension step at 72 °C for 15 min. The forward primers for PCR were designed according ... predena- turation step at 95 °C for 5 min, 35 cycles of two-step amplifications (the first five cycles comprised denaturation at 95 °C for 60 s, annealing at 50...

Ngày tải lên: 18/02/2014, 16:20

... GasCHH-U1 for 3¢ RACE and Pab ⁄ GasCHH-D1 for 5¢ RACE under the following conditions: five cycles of 94 °C for 30 s and 72 °C for 2 min, five cycles of 94 °C for 30 s, 70 °C for 30 s and 72 °C for 2 ... T)TGCCAACCATCTG-3¢ (antisense), corresponding to QMVG(K ⁄ R)K(70–75). After a denaturation step at 94 °C for 3 min, 35 amplification cycles were carried out (94 °C for 30...

Ngày tải lên: 23/03/2014, 07:20

... amounts of core protein 1 and core protein 2 were relatively unaffected. Therefore, the absence of subunit 6 appeared to alter the rates of processing of two of the redox subunits and caused minor ... assembly of the yeast bc 1 complex from the laboratories of Berden [30] and Tzagoloff [31]. It has been demonstrated previously that gene expression, import of proteins into mi...

Ngày tải lên: 19/02/2014, 12:20

... indicated in green for the cofactor ThDP, in blue for the activator ADP, and in orange for the substrate (here PADP). Differ- ent amino acid residues at the substrate binding site are indicated ... A ˚ for 1023 super- imposed Ca atom pairs of 2q27 and 2q29, and rmsd 0.16 A ˚ for 993 superimposed Ca atom pairs of 2q28 and 2q29), indicating that binding of the activator ADP o...

Ngày tải lên: 06/03/2014, 11:20

... respectively. For clarity of presentation, only the most abundant linkage and /or sequence informa- tive ions are schematically illustrated and annotated. The nomenclature for the ion series follows that ... and Mr Sz-Wei Wu for the MALDI-TOF/TOF MS/MS analyses of sugars. Mass spectrometry data were acquired at the NRPGM Core Facilities for Proteomics, Academia Sinica, supporte...

Ngày tải lên: 07/03/2014, 06:20

... whatever the type of cells i.e. either drug- sensitive or drug- resistant, at the steady-state (s) the rate of rhodamine influx (V + ) s is equal to that of rhodamine efflux; (c) for drug- resistant ... V + ,therateofpassiveuptakefor rhodamine, is equal to the number of moles that enter by passive diffusion into one cell per second; V – ,therateof passive efflux for rhodami...

Ngày tải lên: 08/03/2014, 08:20

... molecular genetics data have provided useful information for clinical care of the patients and were an important step towards the understanding of the mechanisms of this disorder. This minireview ... retinoblastoma or other vascular malformations [29,30]. According to this hypothesis, CCM formation would be caused by a complete loss, within affected cells, of the two alleles of...

Ngày tải lên: 15/03/2014, 10:20

... of the underlying biology of lesion formation. In this regard, cerebral cavernous malformation as a genetic disorder offers unique opportunities to understand the biology of vascular malformations. ... blood– brain barrier of a CCM [19] or if inflammatory action is part of the mechanism of pathogenesis leading to formation of aberrant blood vessels. It is intriguing that the mi...

Ngày tải lên: 15/03/2014, 10:20

... Rudnik- Schoneborn S, Bertalanffy H, Sure U & Felbor U (2008) Novel CCM1, CCM2, and CCM3 mutations in patients with cerebral cavernous malformations: in- frame deletion in CCM2 prevents formation of ... (2006) An inven- tory of mucin genes in the chicken genome shows that the mucin domain of Muc13 is encoded by multiple exons and that ovomucin is part of a locus of related gel-f...

Ngày tải lên: 15/03/2014, 10:20