Báo cáo khoa học: Enhancing thermostability of maltogenic amylase from Bacillus thermoalkalophilus ET2 by DNA shuffling pdf

... The Authors Journal compilation ª 2006 FEBS 3345 Enhancing thermostability of maltogenic amylase from Bacillus thermoalkalophilus ET2 by DNA shuffling Shuang-Yan Tang 1 , Quang-Tri Le 1 , Jae-Hoon ... Thermostable and alkalophilic malto- genic amylases of Bacillus thermoalkalophilus ET2. Biocatal Biotransform 23, 79–87. Enhancing MAase thermostability by...

Ngày tải lên: 16/03/2014, 13:20

... a 3-A ˚ dissociation of subunits interacting by a1. The base of dTTP is inserted between the a1-helix of two subunits and is stacked between the rings of Phe18 and Phe34 from the adjacent subunit ... enzyme. The pattern of interaction of ATP with TKs from Gram-negative bacteria remains to be evaluated. O4 of the thymine makes a hydrogen bond with the main- chain nitrogen...

Ngày tải lên: 23/03/2014, 09:21

... of the WT enzyme and between those of G194P and G198P. Thermostability of WT and mutant enzymes The thermostability of the WT and mutant enzymes was determined by measuring residual activity after incubation ... stability of Ochr-MPH; however, replacing G198 of Ochr-MPH with proline did not improve the thermostability. The thermostability of the double point mutant (G1...

Ngày tải lên: 15/02/2014, 01:20

... the efficiency of a parser with a possible trade-off of los- ing correct parses. For example, the noun phrase reading of The dog barks is a correct parse, although unlikely. By blocking the use of barks ... jh5 section of the treebank released with the ERG. This text consists of edited written English in the domain of Norwegian hiking instructions from the LOGON project (Oepe...

Ngày tải lên: 17/03/2014, 02:20

... fraction of bound 32 P-labeled DNA (R) is the ratio of the fraction of bound 32 P-labeled DNA in the presence of the competitor DNA (cpm bound ⁄ cpm total ) to the fraction of bound 32 P-labeled DNA ... efficiency of the reference unmodified DNA and the competitor damaged DNA to the MTase. The K d values were calculated by dividing K r d (see below) by the experi- me...

Ngày tải lên: 19/02/2014, 00:20

... half-life of % 13 and 33 min, respectively. Substrate specificity analysis An analysis of the substrate specificity for DNA of the enzymes shows that they both cleave plasmid DNA, dsDNA and ssDNA as ... calculated as described by Lonhienne et al. [37]. Activation energy, E a , was extracted from the slope of the linear regression curve obtained from an Arrhenius plot of 1 ⁄...

Ngày tải lên: 19/02/2014, 05:20

... exhibit a wide range of structural diversity produced by a sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini- tial origin of structural diversity of skeletons in their ... respectively, by GC-MS. Co-expression of CYP93E1 and b-amyrin synthase in S. cerevisiae yielded olean-12-ene-3b,24-diol. This is the first identification of triterpene hydroxylase c...

Ngày tải lên: 19/02/2014, 07:20

... lm. A B Fig. 5. Expression of iNOS in duodenum tissue of mice. (A) Quanti- fication of iNOS mRNA induction by flagellin in whole tissue and microdissected epithelium from villi assessed by real-time PCR. ... respectively. Specificity of PCR was checked by ana- lyzing the melting curve. Relative mRNA levels were deter- mined by comparing (a) the PCR cycle threshold between cDNA...

Ngày tải lên: 19/02/2014, 16:20

... alignment of the C-terminal extension of PhKc. Sequences were obtained for both vertebrate and nonvertebrates by using sequence from 296 to 386 of PhKc from rabbit muscle. Identities are shown by the ... 1.2 molar ratio of Ca 2+ ⁄ CaM to PhK5 had been reached. The inset shows an expanded view of the indi- cated area of the spectrum. (Bottom) An overlay of spectra from...

Ngày tải lên: 19/02/2014, 17:20

... half-maximal amounts of pPacUSp1 (50 ng, left panel) or increasing amount of Ets-1 with 50 ng of either Sp1 or Sp3. (B) Assay of fgl2 promoter activity upon cotransfection of thresholds amount of both Sp1 ... site of fgl2 mRNA. (A) 5¢-RACE of fgl2 mRNA from macrophages. Stars indicate the 5¢-ter- minus of 33 clones isolated after 5¢-RACE. (B) Primer extension analysis of...

Ngày tải lên: 20/02/2014, 11:20