Báo cáo khoa học: Characterization of a membrane-bound amino

Báo cáo khoa học: Characterization of a membrane-bound aminopeptidase puriﬁed from Acyrthosiphon pisum midgut cells A major binding site for toxic mannose lectins pptx

... Bmo AAX39866 Tni APN4 AAK69605 Sli AAF37559 Hpu APN2 AAK58066 Hvi AAC36148 Pin AAX39865 Tni APN3 AAF01259 Pxy APN3 Q11000 Hvi AAN75694 Har APN2 AAF37560 Hpu APN3 AAF99701 Epo AAD31183 Ldi APN1 AAL83943 ... APN1 AAF08254 Hvi AAN75693 Har APN1 AAF37558 Hpu APN1 AAC33301 Bmo Q11001 Mse A pisum APN CAA66467 Pxy AAX39864 Tni APN2 AAD31184 Ldi APN2 BAA32140 Bmo P91885 Mse APN2 CAA10950 Pxy BA...

Ngày tải lên : 16/03/2014, 12:20

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Tài liệu Báo cáo khoa học: Characterization of phycoviolobilin phycoerythrocyanin-a84-cysteinlyase-(isomerizing) from Mastigocladus laminosus pdf

... the confor- mation of chromophore, to a form suitable for the PVB- PEC-lyase to act on, and unfavourable for PecA to bind spontaneously to PCB. Changes of the conformational equilibria of bile ... His 6 -PecA was decreased 15%. However, in this case a small amount of the ligation/isomerization product, His 6 -a- PECA, was formed (7% as compared to the maximal yield of His 6...

Ngày tải lên : 21/02/2014, 03:20

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Báo cáo khoa học: Characterization of the cofactor-independent phosphoglycerate mutase from Leishmania mexicana mexicana docx

... using TURBOSEQUEST (ThermoFinnigan) database search engine or manually with the help of XCALIBUR software (ThermoFinnigan). Search parameters incorpor- ated a mass difference of 72.00 atomic mass units for N-carbethoxyhistidine ... Virtually all mutase activity measured in the supernatant was vanadate resistant and therefore due to i-PGAM. The supernatant was then passed through 1.5 m...

Ngày tải lên : 16/03/2014, 16:20

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Báo cáo khoa học: Folding of epidermal growth factor-like repeats from human tenascin studied through a sequence frame-shift approach pdf

... quantitative analysis of the RP-HPLC proﬁles showed that the rates of disappearance of the reduced forms are similar but not identical (Fig. 4A) . A ﬁt of experimental data with a three-parameter ... peptides f2–f6 was carried out on the same Gilson chromatographic apparatus using a PrePak Cart ridge 25 · 100 m m (Agilent) casted on a PrepLC Un iversal Base apparatus (Waters)...

Ngày tải lên : 23/03/2014, 13:20

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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... Characterization of the interaction between the plasma membrane H + -ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida ... primers: gatggatcccatATGGGTG TTGAAGTTGTA annealing around the start codon of the Ppi1 ORF and gactcgagATTAGTCGACTTCTTACGC annealing just before the putative transmembrane domain (capital lette...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... 5¢-CTTTAACTTGTTGGGCACTGG CATTG-3¢; NP6, 5¢-TTGATCGATTCTGTCTATGCCC CA-3¢ along with the adaptor primers: AP1 (5¢-GTAATAC GACTCACTATAGGGC-3¢) and AP2 (5¢-ACTATAGGG CACGCGTGGT-3¢). Nested PCR was carried ... PCR was performed in 50 lL containing 1 ng of genomic DNA as template, 0.2 mm dATP, dCTP, dGTP and dTTP, 0.2 lm of upstream primer (OP17: 5¢-GA AAAACCATGGTGAATGAATACCAAGCGACT-3¢ ) and do...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

... Aiba Y, Nakamura K, Namba T, Hirata M, Mazda O, Katsura Y & Narumiya S (1993) Thromboxane A2 receptor is highly expressed in mouse immature thymocytes and mediates DNA fragmentation and apoptosis. ... pGL3e: Prm3 AP)1 *, pGL3b: Prm 3a AP)1 *, pGL3e:Prm 3a AP)1 *, pGL3b:Prm3ab AP)1 *, pGL3e: Prm3ab AP)1 *, pGL3b: Prm3aa AP)1 *, pGL3e:Prm3aa AP)1 *, pGL3b:Prm3aaa AP)1 * and pGL3e:Prm3aaa...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Characterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier pdf

... 5¢-GAGCCCGGATCCACCATGAA GGTCTCAATAATT 3¢;3¢ primer, 5¢-CTGACG GAAT TCTTAAACATTAATGCC 3¢. These primers encoded a Kozak consensus sequence as well as BamHI and EcoRI restriction sites. The PCR-ampliﬁed ... with M. brassicae CSPMbraA6 [32]. We observed also that BrC15-Ac was able to displace ASA, suggesting that brominated fatty acid and ASA both associated with W81 in the same ligand bindin...

Ngày tải lên : 21/02/2014, 03:20

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Báo cáo khoa học: Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae potx

... Journal compilation ª 2011 FEBS 1853 Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae Malea M. Kneen 1 , Razvan Stan 1 , Alejandra Yep 2 , ... an ‘instant’ PDC. It appears that it will be easier to expand the active site to accept larger substrates and, as shown for benzoylformate decarboxylase [16], satu- ration mutage...

Ngày tải lên : 06/03/2014, 00:21

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Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt

... localization and induction by highlight. Mol Microbiol 69, 231–244. 41 Prado-Cabrero A, Estrada AF, Al-Babili S & Avalos J (2007) Identiﬁcation and biochemical characterization of a novel carotenoid ... M, Azulay Y, Portnoy V, Wasserman B, Bar E, Meir A, Burger Y, Hirschberg J, Schaffer AA, Katzir N et al. (2006) Functional characterization of CmCCD1, a carotenoid cleavage d...

Ngày tải lên : 07/03/2014, 03:20

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