Báo cáo khoa học: Functional characterization of ecdysone receptor gene switches in mammalian cells docx

... participates in the functioning of this VP16-EcR gene switch. However, the use of EcR DNA binding domain and EcRE raises the possibilities of endogenous steroid receptors of the host cells binding to ... because of the inadequate understanding of molecular mecha- nisms of their function in vivo. An insect hormone receptor based gene regulatory system, ecdysone rec...

Ngày tải lên: 16/03/2014, 12:20

... concentration of the 16 kDa protein subunit) increased linearly with increasing fractional saturation of the metal site in Bxe_A2876, the k cat determined from the molar concentration of Fe 2+ -containing ... one of the following substrates was offered to a protein solution containing 7 lm metal sites: xanthine in the presence of 2-ketoglutarate; cat- echol; or quercetin. Howeve...

Ngày tải lên: 18/02/2014, 06:20

... of front-end desaturases are present in each of these trypanosomatid proteins, including the three highly conserved histidine boxes and the cytochrome b5 N-terminal domain (Fig. 1). Interestingly, ... recognize. They contain a fused cytochrome b5 as an N-terminal domain and three histidine boxes in the desaturase (C-terminal) domain. The third histidine box has the QX 2 HH instead of...

Ngày tải lên: 07/03/2014, 12:20

... 90% of artemin-containing cells endured a 30 min heat shock, as opposed to only 17% of those lacking the protein (Fig. 4A). In comparison, 55% of cells containing arte- min survived incubation in ... staining intensity on western blots and in comparison to the protein in cyst extracts, suggesting that it protected transfected cells in a way other than by indiscriminate b...

Ngày tải lên: 16/03/2014, 11:20

... Within the more general context of protein evolution, this finding reveals how important the surface electrostatic features of molecules a re for their functional interactions within the living cells. Acknowledgements Research ... in a wide variety of organisms from an evolutionary point of view, thereby yielding a hierarchy of kinetic models with a significant increase in effi...

Ngày tải lên: 16/03/2014, 16:20

... superfamily of nuclear receptors. Like other members of the family, HNF-4a is characterized by the presence of two well-conserved functional domains, the DNA-binding domain (DBD) consisting of two zinc-finger ... motifs that specifically bind to DR1 elements found in promoters of target genes, and the ligand-binding domain (LBD), consist- ing of a ligand-binding site, dimerizati...

Ngày tải lên: 23/03/2014, 10:21

... other eIF2a kinases, the mutation of the invariant lysine in the kinase subdomain II impaired the ability of DPERK to rescue growth of yeast lacking GCN2. Moreover, the use of strain J82 (Dgcn2 ... Hinnebusch, A.G. (2001) The tRNA-binding moiety in GCN2 contains a dimeri- zation domain that interacts with the kinase domain and is required for tRNA binding and kinase activation. EM...

Ngày tải lên: 31/03/2014, 07:20

... epitopes in the N-terminal (ATPase) domain while the remaining four mAbs, obtained with intact MalK, bind to the C-terminal (regulatory) domain. For precise determination of the epitopes overlapping ... N-terminal domain. Thus, these findings clearly suggest that MalT binds to the transport complex by interaction with the C-terminal domains of the MalK subunits. Enzyme IIA Glc eliminates...

Ngày tải lên: 31/03/2014, 09:20

... or D , D -Hep/ L , D -Hep during in vivo growth and pathogenesis are not understood. It may be that C-reactive protein binds and mediates killing efficiently of organisms expressing the PCho-containing glycoforms and ... determination of 5-N-acetyl neuraminic acid in lipopolysaccharides of Haemophilus in uenzae: a survey of 24 non- typeable H. in uenzae strains. Carbohydr. Res. 3...

Ngày tải lên: 08/03/2014, 02:21

... here in the form of SI. An SI > 2 was considered to be significant: SI = (D 540 nm of activated cells – D 540 nm of inactivated cells) ⁄ D 540 nm of inactivated cells. Cytokine assay – determination ... Rv3020c contains histidine in place of tryptophan in the WXG motif, which induces the formation of a small helix that joins the N-terminal and C-terminal domains [13]....

Ngày tải lên: 22/03/2014, 16:21