Báo cáo khoa học: Concerted mutation of Phe residues belonging to the b-dystroglycan ectodomain strongly inhibits the interaction with a-dystroglycan in vitro pot
Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt
... determined the X-ray crystal structure of a truncated form of importin-a lacking the importin-b binding domain, bound to a CLIC4 NLS peptide. The NLS peptide binds to the major binding site in an extended ... at its location with the hydroxyl group reaching the im- portin-a main chain near the C-terminus of the ARM1 H3 helix. Most of the hydrophobic contacts...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc
... able to interact with the N-terminus of the peptide itself but not with the enzyme. Interestingly, a hydrophobic interactionofthesidechainofMet1ofTat(1–9)withthe phenyl ring of Ala-Pro-pNA may hinder ... Ile8 of the peptide interacting with the Tyr752 hydroxyl group of the enzyme. A hydrophobic interactionofthesidechainofMet1ofTat(1–9)withthe phenyl ring of the...
Ngày tải lên: 20/02/2014, 11:20
Báo cáo khoa học: Heterologous synthesis of cytochrome c¢ by Escherichia coli is not dependent on the System I cytochrome c biogenesis machinery ppt
... corresponding to the PHCP protein sequence of the 31st to 40th residues (Fig. 2), and a gene-spe- cific forward primer, PHcp03fw, corresponding to the sequence of the 45th to 53rd residues. The resulting ... protein was able to heter- ologously synthesize the PHCP and PH c 552 proteins, confirming that the observed difference between the two proteins in the...
Ngày tải lên: 06/03/2014, 00:20
Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf
... aa; nine aa in total) were located near the interface of the physiological dimer. The C- terminal domain may contain structural features involved in protein–protein interactions to form the physiological ... differences in the interactions across the domain interface. All the resi- dues involved here seem to be unrelated to the Tris- binding site residues (incl...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: Secondary structure of lipidated Ras bound to a lipid bilayer pptx
... mea- sured in solution. Furthermore, it resembles the mean of an ensemble of 20 structures and thus indicates the dynamics of the protein, leading to changes in second- ary structure according to the ... flow. Protein incorporation After the formation of the model membrane, protein incor- poration was initiated by mixing the protein into the sample solution on...
Ngày tải lên: 07/03/2014, 04:20
Báo cáo khoa học: Identification of crucial residues for the antibacterial activity of the proline-rich peptide, pyrrhocoricin pot
... reflected the presence of vital residues for the binding and flanking residues likely needed to maintain the structural integrity at the interaction sites (Fig. 7). The major drop in the fluorescence ... hence the interactive domain with the bacterial target protein, the C-terminal half aided the delivery of the pharmacophore into the interior of cells....
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf
... elements. The topological arrangement of these elements is usually bababba, similar to the thioredoxin fold of other GSH-binding or cysteine-binding proteins. The H-site is entirely helical, with ... assistance interactions to the developing charge of the r-complex o-nitro group [33]. The comparison of the crystal structure of the rat M1- 1 GST in complex wi...
Ngày tải lên: 15/03/2014, 09:20
Báo cáo khoa học: Recent contributions of in vitro models to our understanding of hepatitis C virus life cycle pdf
... of CD81 in the binding process was further illustrated by the poor binding inhibition achieved in the presence of mAbs directed against CD81. In contrast, heparin sul- fate seemed to mediate this interaction ... Moreover, the chi- meric nature of the viruses and the intrinsic character- istics of the Huh7 cell line (its nonpermissiveness to infection with H...
Ngày tải lên: 16/03/2014, 05:20
Tài liệu Báo cáo khoa học: Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation pdf
... 7.4) to determine the protein thermostability. The purified proteins were stored at )20 °C in aliquots until use. The purity of the proteins was analyzed by SDS ⁄ PAGE followed by staining with ... MPH using pro- tein engineering. The two main protein-engineering strategies that can be used to increase protein thermostability are rational design and random mutagenesis [10–1...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx
... revealed the importance of conserved residues in the vicinity of the active site that serve to position the functional K12 residue. The network of key interactions spans the interacting subunits. The ... the backbone CO of F102. Fur- thermore, the orientation of the side chain of the two residues brings the guanidinium plane and the aro- matic rin...
Ngày tải lên: 18/02/2014, 11:20