Báo cáo khoa học: Biochemical characterization of rice trehalose-6-phosphate phosphatases supports distinctive functions of these plant enzymes potx

... characterization of HpSDH demonstrates its activity with k cat of 7.7 s )1 and K m of 0.148 mm toward shikimate, k cat of 7.1 s )1 and K m of 0.182 mm toward NADP, k cat of 5.2 s )1 and K m of ... Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China 2 School of Pharmacy, East China University...

Ngày tải lên: 19/02/2014, 05:20

... 2006 FEBS Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism Matthias Mack 1 , Ute Schniegler-Mattox 2 , Verena Peters 3 , Georg F. Hoffmann 3 , ... humans, isolated deficiencies of each of the six dif- ferent steps within the leucine degradation pathway (Fig. 1) cause their own characteristic disease [1]. The enzymes of this...

Ngày tải lên: 19/02/2014, 07:20

... of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalytic cycle. The substrate binding site of the type II enzyme remains veiled. However, a patch of ... These enzymes were found to be dependent on NADPH and Mg 2+ as cofactors. Anaerobic conditions were not tested in these studies. In contrast to these findings [52–54], the catalytic...

Ngày tải lên: 19/02/2014, 13:20

... specificity and enzymatic properties of USP7 As part of the characterization of USP7 biochemical properties, we have measured its kinetic parameters for the hydrolysis of ubiquitin C-terminal 7-amido-4-meth- ylcoumarin ... molar ratio of 100 : 1 in a final volume of 150 lL. Reactions were stopped by addition of one volume of 2 · Laemmli sample buffer, followed by boiling at...

Ngày tải lên: 07/03/2014, 05:20

... estimated from fluorimetric cofactor analyses of the two recom- binant enzymes was in the range 0.2–0.3 mol flavin per mol protein. Kinetic characterization Activity measurements of CaDHODH and DNCaD- HODH ... amino-acid residues of the C. albicans protein. (B) Alignment of the catalytic centre of the recombinantly expressed C. albicans DHODH and amino-acid sequences of differen...

Ngày tải lên: 07/03/2014, 12:20

... Winter et al. Biochemical characterization of annexin B1 FEBS Journal 273 (2006) 3238–3247 ª 2006 The Authors Journal compilation ª 2006 FEBS 3247 Biochemical characterization of annexin B1 from Cysticercus ... Yusof 1 , Erning Gao 1 , Hong-Li Yan 2 and Andreas Hofmann 1 1 Institute of Structural & Molecular Biology, School of Biological Sciences, The University of Edinb...

Ngày tải lên: 07/03/2014, 12:20

... of the LOX and hydroperoxide lyase activities. Further studies are in progress to clarify the role of these enzymes in the production of volatile compounds, which are important constituents of ... Mita 1 1 Institute of Sciences of Food Production C.N.R. Section of Lecce, Italy; 2 John Innes Centre, Norwich, UK Plant lipoxygenases (LOXs) are a class of dioxygenases which...

Ngày tải lên: 21/02/2014, 00:20

... fruiting of V. volvacea on straw The duration of the developmental cycle of V. volvacea when the mushroom was grown on rice- straw ÔcompostsÕ was approximately 23 days. For the purpose of this ... fluids of V. volvacea only during the latter stages of primary growth. In order to better under- stand these effects at the molecular level, we have now purified and characterized one...

Ngày tải lên: 07/03/2014, 15:20

... similar to that of other well- characterized members of PUF family. These proteins must bind to transcripts in the plant and regulate their translation and ⁄ or turnover. A large number of APUM target ... therefore predict that various PUF of A. thalina may be involved in many different processes in the plant. RNA-binding capacity of the APUM proteins pfam analysis of all pu...

Ngày tải lên: 18/02/2014, 06:20

... in the course of the reaction. The principle of the assay is that the presence of a SOD inhibits the reduction of cytochrome c. This was vali- dated by adding a total of 2.5 U of commercial SOD ... comparison of nonheme metal- dependent enzymes. Fig. S2. Overlay of the homology model of Bxe_A2876 (white) with the template crystal structure of RgCarb (RgCAR) (yellow) and...

Ngày tải lên: 18/02/2014, 06:20