Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf

... Electron-density map of the dCMP -binding region for the R188M CMP kinase variant. The F o ) F c omit map calculated for the dCMP–R188M CMP kinase complex in the absence of the dCMP model is green. The contour ... with the amino group and the N3 atom of the cytosine (Fig. 1). This study uses site-directed muta- genesis to further explore the contribu...

Ngày tải lên: 16/03/2014, 10:20

... solutions. The ligand bound was interpreted as GMP because the N2 of the base makes a hydrogen bond to a main chain car- bonyl. Binding of a xanthine base from XMP in the same position, would lead to the ... guanine and hypoxanthine bases, respectively, indicating that Asp137 functions as a catalytic base [12]. However, a tight binding of N7 of the purine ba...

Ngày tải lên: 15/02/2014, 01:20

... shown indicate the starting and ending residues of the secondary structural elements. (C) The domain-swapped dimer of St SurE. The A and B subunits are shown in green and blue, respectively. The ... determined and con- firmed by comparison with the S. typhimurium genome. The cloning strategy resulted in 14 additional amino acids from the vector at the N te...

Ngày tải lên: 18/02/2014, 14:20

... 168–174 are missing; in chain F, residues 1 and 168–175 are missing. All amino acid residues were present in the E chain, and part of the His-tag was observed in the B chain. A few amino acid residues ... Superposition of UpUMPK (green) on top of E. coli UMPK (blue) at the active site. (B) Binding of UMP to E. coli UMPK and the location of amino...

Ngày tải lên: 18/02/2014, 16:20

... candidates. Receptor binding of PDGFs The PDGFs bind to the protein tyrosine kinase receptors PDGF receptor-a and -b. These two recep- tor isoforms dimerize upon binding the PDGF dimer, leading to three ... heparin- binding domain in the C-terminal region of the growth factor domain but the exact residues responsible for the binding have not been identified [25]...

Ngày tải lên: 19/02/2014, 07:20

... of the molecule involving a-helix A, the nearby loop, the first and last turns of helices E and F, and the C-terminus helices I and J. In addition, there is a lysine-rich N- and C-ter- minus, in ... anti-parallel to the same strand of a second monomer, allowing for the formation of the dimer. The surface of interaction between monomers also involves port...

Ngày tải lên: 06/03/2014, 00:21

... within the a-crystallin domain, better understand the conse- quences of amino acid substitutions, and identify pro- tein regions involved in oligomer assembly. The four modified a-crystallin domain ... resem- blance, and the functional analysis mentioned previ- ously, indicate data obtained by examining bacterially produced p26 are indicative of the protein synthesiz...

Ngày tải lên: 07/03/2014, 12:20

... hydrolysis of the nucleotide. To investi- gate the in uence of the binding and ⁄ or hydrolysis of ATP on the interaction of the AAA-peroxins, Pex1p and Pex6p carrying mutated ATP -binding sites ... do interact in vivo. We assigned their corresponding binding sites and elucidated the importance of ATP -binding and -hydrolysis of Pex1p and Pex6p for th...

Ngày tải lên: 07/03/2014, 16:20

... and the cysteine knot is boxed. Also boxed are the transmembrane domain, the ATP binding site, the L45 loop and the serine ⁄ threonine kinase domain. (B) The implied amino acid sequence of Cg- TGFbsfR2 ... the amino terminal end and C2 defines the domain closest to the cell membrane. A comparison of the inferred amino acid sequence of the C1 and...

Ngày tải lên: 07/03/2014, 21:20

... Ca 2+ binding and homodimerization of Iba1 and Iba2. Furthermore, F-actin binding and cross-linking assays were performed with both human Iba proteins, and their role in bacterial invasion was investigated. Results Crystal ... conformational change of EF-hand proteins upon Ca 2+ binding [18]. Thus, it may be concluded that EF-hand 1 of the Iba proteins is not capable...

Ngày tải lên: 16/03/2014, 06:20