Báo cáo khoa học: Substrate and inhibitor specificity of Mycobacterium avium dihydrofolate reductase pptx

... compilation ª 2007 FEBS Substrate and inhibitor specificity of Mycobacterium avium dihydrofolate reductase Ronnie A. Bo ¨ ck 1, *, Jose L. Soulages 2 and William W. Barrow 1 1 Department of Veterinary Pathobiology, ... (1983) Directed mutagenesis of dihydrofolate reductase. Science 222, 782–788. 2 Hartman PG (1993) Molecular aspects and mechanism of action of...

Ngày tải lên: 16/03/2014, 10:20

... localization of the different epimers. Taken together, these results allow a discus- sion of the existence of enzyme(s) converting l-to d- residues in the peptide chain in terms of substrate specificity and ... the elution zone of CHHs and VIHs from lobster sinus glands was per- formed: 10 lL aliquots of each fraction were pipetted in triplicate into the wells of a microt...

Ngày tải lên: 18/02/2014, 11:20

... mRNA and protein levels of key pro- fibrotic proteins like PDGFR-a and -b, TGFb1, and TIMP-1 and -2 in the liver of transgenic mice. PDGF-C treatment increased both mRNA and protein levels of the ... model of the PDGF-C growth factor domain indicates the disulfide bridges in PDGF-C to consist of Cys250 and 294, Cys280 and 335, and Cys287 and 337, and the inter- mon...

Ngày tải lên: 19/02/2014, 07:20

... structures of the tandem GAF domains of PDE2 and cyaB2 do not reveal how cNMP specificity is enco- ded, we generated chimeras between the tandem GAF domains of cyaB1 and PDE2. Both bind the ligand in ... [2,3]. The lig- and for the tandem GAF domains of PDE2, PDE5, PDE6, and PDE11 is cGMP and that for PDE10 is cAMP. Usually ligand-binding enhances catalytic activity [4–6]....

Ngày tải lên: 19/02/2014, 02:20

... (1977) Mechanism of action of adenosylcobalamin: glycerol and other substrate analogues as substrates and inactivators for propanediol dehydratase – kinetics, ste- reospecificity, and mechanism. ... (DR), and GDR (GR) are indicated with arrowheads to the right of the gels, and their complexes 1–4 to the left. Bands 1 and 2 correspond to 1 : 2 and 1 : 1 DD–DDR complexes, r...

Ngày tải lên: 16/03/2014, 05:20

... synthesized is 35% of the total amount of plasma membrane proteins, in comparison with the level of H + -ATPase which represents 20–50% of the total amount of plasma membrane proteins [23,24], and assuming ... 5537 mechanisms of adaptation (storage mobilization, expression of exporter, adaptation of membrane per- meability), their timing and the modeling of biomass produ...

Ngày tải lên: 07/03/2014, 04:20

... D esign and synthesis of a p otent c yclic ana- logue of the myelin basic p rotein epitope MBP72-85: importance of the Ala81 carboxyl group and of a cyclic conformation for induction of experimental ... side chain of Arg78 (H g ) and Glu82 (H c1 )and the medium-range NOE between the side chains of Asp81 (H b ) and Arg78 (H g and H c )]. In addition, Glu82 i nteracts...

Ngày tải lên: 07/03/2014, 16:20

... Isolation and purification of the pro- tein and cloning and sequencing of its operon. J Biol Chem 267, 14193–14203. 27 Uhlmann H, Kraft R & Bernhardt R (1994) C-Terminal region of adrenodoxin ... the microsomal type of P450s like P450 BM3 [24] and are reduced by a diflavin reductase. This is the first report on the heterologous expression and purification of all component...

Ngày tải lên: 07/03/2014, 16:20

... factor of 18.8% and an R free of 22.3% with over 90% of the residues in the most favored region of a Ramachandran plot (Table 2). The resulting model is depicted in Fig. 2. The Ca backbones of the ... b-strands are labeled. Fig. 3. B-factor plots of the main chains of the two molecules of CDase in the asymmetric unit. The averages of molecules A (solid line) and B (broke...

Ngày tải lên: 08/03/2014, 02:20

... 2011 Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia coli transthyretin-related protein Erik Lundberg 1 , Anders Olofsson 2 , Gunilla T. Westermark 3 and ... S & Shoham M (1999) Crystal structure of a thermophilic alcohol dehydroge- nase substrate complex suggests determinants of substrate specificity and thermostabili...

Ngày tải lên: 16/03/2014, 01:20