Báo cáo khoa học: Tumor suppressor p16INK4a ) modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells ppt

... presentation of galectin-1- binding sites in p16 INK4a -expressing cells and acquisition of anoikis sus- ceptibility associated with the fibronectin receptor. Induction of anoikis by galectin-1 In order to ... down-regulation of A B C Fig. 6. Role of galectin-1 in p16 INK4a -mediated anoikis induction. Quantification of p16 INK4a and galectin-1 presenc...

Ngày tải lên: 16/03/2014, 10:20

... (200 7) Tumor suppressor p16 INK4a : modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate- dependent induction of anoikis in pancreatic carcinoma cells. ... Gal-1-stimulated anoikis and Gal-1 binding. (A) Determination of Gal-1-stimulated (100 lgÆmL )1 ) anoikis in p16 INK4a -restituted ce...

Ngày tải lên: 29/03/2014, 21:20

... We did observe an increase in the TDH1 mRNA level, encoding one of the GAPDH isoenzymes, in line with earlier reports of induction of this transcript in heat-shocked cells and under glucose starvation ... numbers increased over time (Fig. 1B). However, the cell num- ber increased exponentially in cells shifted to glucose excess in the continued presence of nitrog...

Ngày tải lên: 18/02/2014, 06:20

... colors) and H298E (orange, dark colors). (D) H298E. (E) H298E–C12. (F) H298Q. (G) H298Q–C12. (H) Superimposi- tion of H298Q and H298Q–C12. In (A, B) and (D–G), water molecules (red spheres) within ... decarboxylation [9]; (e) two threonines (T300 and T30 2), speculated to contribute to ACP binding during malonyl-ACP decarboxylation [2,3], and (f) a phenyl- alanine (F39 2)...

Ngày tải lên: 19/02/2014, 08:20

... cm )1 in the spectra of both chitosan and LMWC indicated the absence of free -OH groups and the involvement of both -OH… 3and -CH 2 OH…6 in intra- and intermolecular hydrogen bonding (Fig. 2) [31]. ... shifting ofthesameinLMWC(3368cm )1 ), indicating a decrease in the ordered structure. This is probably due to the decrease in the DA (Table 1) of LMWC, w...

Ngày tải lên: 19/02/2014, 12:20

... produces extension of oligomannosides bound to lipid intermediates) as in Fig. 4D (0% of Man5 population); (b) incubation of cells in the culture medium (5 m M Glc) which considerably reduces the labeling. In ... bound to LLO (A, B, C and D) and glycoproteins (E, F, G and H) were ana- lyzed by HPLC as described in Experimental procedures. (D) and (H) correspond to...

Ngày tải lên: 16/03/2014, 16:20

... cm )1 ) of native purple membrane (solid line), purple membrane after mild exposure to Triton (0.1% Triton, 7 min; dashed line), and Triton-treated purple membrane reconstituted in the absence of ... 1680 cm )1 (the amide I of b-turn structures), 1640 cm )1 (the amide I of random coil and b-sheet structures), 1545 cm )1 (the amide II of a-helical A mode),...

Ngày tải lên: 17/03/2014, 03:20

... cathepsin G, and pro- teinase 3: evidence for distinct cleavage sites within the aminoterminal domain of the thrombin receptor. Blood 89, 1944–1953. 33 Bastos M, Maeji NJ & Abeles RH (199 5) Inhibitors ... and mast cells, respectively, although CG may also be found in mast cells [4]. The understanding of their distinctive roles in inflammatory events involving both neutro...

Ngày tải lên: 28/03/2014, 22:20

... member of the ADAM (a disintegrin and metalloproteinase) family of type I membrane proteins and mediates the ectodomain shedding of various membrane- anchored signaling and adhesion proteins. TACE ... effects. HEK293 cells express G protein-coupled muscarinic receptors and agonist binding results in an intracellular increase of the second messengers inositol 1,4,5-trisph...

Ngày tải lên: 08/03/2014, 02:20

... septic shock and are also defective in the production of the active cytokines interleukin-1b, interleukin-18 and interleukin-33 [6–9]. Involvement of caspase-1 in TNF-a-induced cytotoxicity has been ... (199 9) Defective binding of IRFs to the initiator element of interleukin-1beta-converting enzyme (ICE) promoter in an interferon-resistant Daudi subline. FEBS Lett 450,...

Ngày tải lên: 16/03/2014, 05:20