Báo cáo khoa học: Nucleosome positioning in relation to nucleosome spacing and DNA sequence-specific binding of a protein doc

... epidermal growth factor receptor (EGFR) is com- posed of an extracellular ligand -binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. The binding of a ligand to the ... treatment of intestinal epithe- lial cells with gefitinib results in a dramatic increase in apoptosis and activation of the intrinsic apoptotic pathway via trafficking...

Ngày tải lên: 16/02/2014, 09:20

... of the R3 pair bound to L1 and L2. Turning a gene into an active state from a state of inactivation often involves binding of a single activator molecule to its site in a repressive chromatin structure [37]. ... proteins are the dominant determinants for nucleosome positioning and spacing. Several exam- ples of nucleosome positioning due to the binding...

Ngày tải lên: 16/03/2014, 10:20

... Terra WR & Ferreira C (2001) Amino acid residues involved in substrate bind- ing and catalysis in an insect digestive b-glycosidase. Biochim Biophys Acta 1545, 41–52. 21 Ferreira AHP, Marana ... be developed to establish the contribution of each resi- due in the aglycone -binding site to the binding of diverse types of aglycones. Furthermore, these data may be of pa...

Ngày tải lên: 18/02/2014, 17:20

... from Caveolin-1 cDNA are GCAAGTGTACGACGCGCAC and AACCAGAAGGGACACACA, respectively. As negative controls (scrambled shRNAs), shRNAcontrol 1 (TAGCGACTAAACACATCAA), shRNAcontrol 2 (TATA GCGACTAAACACATCAA) and ... for Caveolin-1 and P2X 7 of Caveolin-1 shRNA-treated E10 cells revealed a partial loss of colocalization of P2X 7 , with a remaining low amount of Caveolin-1 protein...

Ngày tải lên: 19/02/2014, 00:20

... 5¢-ATCGACGGGCCTGACTCAT-3¢, 5¢-CAACATTGAGCCCAGCAATAAC-3¢,5¢-CAGCTAT TTAAGCCGTCTTTTCCA-3¢,5¢-GATAGATGCAGAGC CACCAAAGA-3¢,5¢-CGGACATGAGAGAGCAAAGT CA-3¢,5¢-CAGCTGCAAATTATGGTGAAG-3¢ and 5¢- ACCCGACGGTGGTGACTACA-3¢, were ... 5¢-VIC-CAG TACCTTCCAGCAGATGTGGATCGC-TAMRA-3¢,5¢- FAM-CCAGCTTACTTACTTCAATGATGCTCAAAGG C-TAMRA-3¢,5¢-FAM-CTATGCAAGGTCTCAGTCAG GCTCGGC-TAMRA-3¢,5¢-FAM-ATGCTAATGTGGC TCCAGTTTGCCT...

Ngày tải lên: 07/03/2014, 21:20

... that has not been found to date in bacterial polysaccharides. Studies of binding of mAb 2625 to the N-methylated legionaminic acid derivatives is described in the accompanying paper [26]. MATERIALS ... was applied to stereo- chemical analysis of the N-methylated acetimidoylamino (acetamidine) groups in 2 and 3, which may occur as stereoisomers due to a partial double...

Ngày tải lên: 08/03/2014, 10:20

... RNA-cleaving activity in a self-cleaving DNAzyme. The DNAzyme with DNA- cleaving and RNA-cleaving activities was constructed by incorporating the catalytic domain of 8–17 variant DNAzyme into the ... insights into the engineering of DNAzymes. Experimental procedures Materials The DNA sequences (PL DNAzyme, 5¢-GAATTCTAATAC GACTCAGAATGAGTCTGGGCCTCTTTTTAAGAAC-3¢; 8–17 variant DNAzy...

Ngày tải lên: 16/02/2014, 15:20

... (KD2), a trans- membrane domain, and a cytoplasmic domain (Fig. 1A) . A human splice variant containing an extra 16 amino acids inserted between KD1 and the LDL Molecular interactions of HGFA with inhibitor ... The AB CD Fig. 1. Conformational states of the HGFA active site region. (A) Cartoon of HGFA and HAI-1 domain architectures. HGFA contains a heavy chain (A- chain) d...

Ngày tải lên: 15/03/2014, 11:20

... phPGK1–5aa–cerulean were constructed using the primers 5¢-CCGGA ATTCC AATGT CGCTT TCTAA CAAGC T-3¢ and 5¢-GGCGG ATCCA TAATA TTGCT GAGAG CATCC A- 3¢, and 5¢- CCGGA ATTCC AATGT CGCTT TCTAA CAAGC T-3¢ and ... the cytoplasmic area (in the case of cell samples with coexpression of GAPDH) of cells, and are expressed as mean ± standard deviation. n, number of cells examined. Prote...

Ngày tải lên: 16/02/2014, 09:20

... mutual effects in fusion proteins containing an intrinsically disordered and a globular protein Ilaria Sambi 1 , Pietro Gatti-Lafranconi 1 *, Sonia Longhi 2 and Marina Lotti 1 1 Dipartimento di ... an N-terminal hexahistidine tag was obtained by PCR using the pET2 1a ⁄ PNT- H6 plasmid [30] as the template. The forward primer (5¢-TACCGTTAACATCGATATGCATCATCATC ATCATCATGC-3¢) was desi...

Ngày tải lên: 18/02/2014, 04:20