Báo cáo khoa học: Ligand binding and antigenic properties of a human neonatal Fc receptor with mutation of two unpaired cysteine residues docx
Báo cáo khoa học: Ligand binding and antigenic properties of a human neonatal Fc receptor with mutation of two unpaired cysteine residues docx
... 4097–4110 ª 2008 The Authors Journal compilation ª 2008 FEBS Ligand binding and antigenic properties of a human neonatal Fc receptor with mutation of two unpaired cysteine residues Jan T. Andersen 1 , ... human HC variants. Table S3. Numbers of cysteine residues of FcRn HCs across species a . Table S4. Numbers of cysteine residues of non...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: GTP binding and hydrolysis kinetics of human septin 2 pot
... HPLC analysis of bound nucleotides. GTP hydrolysis assay and steady-state kinetics The initial velocity of GTP hydrolysis was measured by a radioactive assay. It was started by the addition of ... presence of [ 32 P]-ATP as described in the methods and then autoradiographed. (B) In-gel kinase assays show that commercial casein kinase II (left panel, right lane) gave bands compar...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Ligand binding promotes prion protein aggregation – role of the octapeptide repeats potx
... Xiao G, Grassi J et al. (2006) Prion pro- teins with insertion mutations have altered N-terminal conformation and increased ligand binding activity and are more susceptible to oxidative attack. ... of fatal neurodegenerative disease in humans and animals. It is believed that all prion diseases are caused by the conversion of a nor- mal cellular prion protein (PrP C ) to a...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: Oxygen binding and its allosteric control in hemoglobin of the primitive branchiopod crustacean Triops cancriformis pdf
... interval of L was calcu- lated from best-fit values and the standard errors of l T , l R , and L according to the law of error propagation [64]. Statistical analysis Unless otherwise stated, data are ... F-statistics predicted the increase in the vari- ance (or the SSR) of a fit that is associated with one standard deviation of the parameter. Acknowledgements The technical ass...
Ngày tải lên: 23/03/2014, 09:20
Báo cáo khoa học: DNA binding and partial nucleoid localization of the chloroplast stromal enzyme ferredoxin:sulfite reductase pptx
... shift assay A double-stranded 40-mer DNA probe was prepared by annealing the oligonucleotide 5¢-AGTCTAGACTGCAG TTGAGTCCTTGCTAGGACGGATCCCT-3¢ and its com- plementary strand. A 20-mer dsDNA probe was ... 1146–1157. 59 Sasaki N, Kuroiwa H, Nishitani C, Takano H, Higashiyama T, Kobayashi T, Shirai Y, Sakai A, Kawano S, Murakami-Murofushi K et al. (2003) Glom is a novel mitochondrial DNA...
Ngày tải lên: 30/03/2014, 08:20
Báo cáo khoa học: DNA-binding and transcription characteristics of three cloned sigma factors from mustard (Sinapis alba L.) suggest overlapping and distinct roles in plastid gene expression doc
... 5¢-GAGAAACAAGTGATACGTTGGA GA-3¢ and 5¢-TTTCCTGAATGCAGATGACTCTAT-3¢ from AtSig2 and 5¢-GTAGAGATGAACTGGTGAA AAGCA-3¢ and 5¢-AAAACTTGTAACCCCTTGTGT GAT-3¢ from AtSig3, were used for PCR-amplification from total S. alba DNA. ... sequence tags with sequence similarity to the coding regions of the AtSig2andAtSig3 genes from Arabidopsis thaliana (GenBank accession numbers AC003981 and N970...
Ngày tải lên: 31/03/2014, 07:20
Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
... exogenous aquacob(III)alamin to cob(II)alamin [Yamada K, Gravel RA, TorayaT & Matthews RG (2006) Proc Natl Acad Sci USA 103, 9476–9481]. Here, we report the expression and purification of human methionine ... domain of neuronal nitric oxide synthase) to: (a) stimulate formation of holo-MS from aquacob(III)alamin and the apo-form of MS; and (b) reactivate the inert cob(II)a...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: The antibacterial and antifungal properties of trappin-2 (pre-elafin) do not depend on its protease inhibitory function pptx
... influenzae, Streptococcus pneumoniae, Branhamella catarrhalis and the pathogenic fungi Aspergillus fumigatus and Candida albicans, in addition to P. aerugin- osa and S. aureus. A similar antimicrobial ... N-terminal domain and is comparable to that of human defensins and human lysozyme. Elafin and trappin-2 are both antimicrobial against S. aureus and P. aeruginosa [14,15]...
Ngày tải lên: 18/02/2014, 17:20
Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
... L, Hamid Q & Elias JA (2004) Acidic mammalian chitinase in asthmatic Th2 inflammation and IL-13 pathway activation. Science 304, 1678– 1682. 4 Kasprzewska A (2003) Plant chitinases ) regulation ... 1084–1092. 35 Kawase T, Yokokawa S, Saito A, Fujii T, Nikaidou N, Miyashita K & Watanabe T (2006) Comparison of enzymatic and antifungal properties between family 18 and 19 chit...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
... bond network around ArgB263 provide no reasonable explanation of the PA activity with substrates like PhAc-pAB, PhAc-mAB, PhAc-oAB vs. phenylacetyl 4-nitroanilide (PhAc-pNA), NIPAB and iso-NIPAB, ... Distances (A A ˚ ) between polar substrates groups (CO 2 – and NO 2 ) and the two ArgB263 and ArgA145 residues of penicillin acylase as a result of AM1 optimization of th...
Ngày tải lên: 16/03/2014, 16:20