Báo cáo khoa học: Ligand binding and antigenic properties of a human neonatal Fc receptor with mutation of two unpaired cysteine residues docx

... 4097–4110 ª 2008 The Authors Journal compilation ª 2008 FEBS Ligand binding and antigenic properties of a human neonatal Fc receptor with mutation of two unpaired cysteine residues Jan T. Andersen1, ... human HC variants.Table S3. Numbers of cysteine residues of FcRn HCsacross species a .Table S4. Numbers of cysteine residues of nonclassicalMHC class I HC a .This material is available as ... the BIAevalutionWizard. The heterogeneous ligand- binding modelassumes that two parallel and independent interactions(KD1 and KD2) take place between the ligand and the receptor, and the...

Báo cáo khoa học: GTP binding and hydrolysis kinetics of human septin 2 pot

... HPLC analysis of bound nucleotides.GTP hydrolysis assay and steady-state kineticsThe initial velocity of GTP hydrolysis was measured by a radioactive assay. It was started by the addition of ... presence of [32P]-ATP as described in the methods and thenautoradiographed. (B) In-gel kinase assays show that commercial casein kinase II (left panel, right lane) gave bands comparable to ... were washed three times with HKA buffer, and the cell lysate was added along with NaCl to a ﬁnal concentration of 150 mm. The mixtureswere rotated at 4 °C for 1–2 h and washed ﬁve times with ristocetin-induced...

Báo cáo khoa học: Ligand binding promotes prion protein aggregation – role of the octapeptide repeats potx

... Xiao G, Grassi J et al. (2006) Prion pro-teins with insertion mutations have altered N-terminalconformation and increased ligand binding activity and are more susceptible to oxidative attack. ... of fatal neurodegenerativedisease in humans and animals. It is believed that allprion diseases are caused by the conversion of a nor-mal cellular prion protein (PrPC) to a pathogenic and infectious ... Comparison of the aggregation of rPrP enhanced by hepa-rin, low molecular mass heparin (LMW heparin, 3 kDa) and heparindisaccharide. rPrP (5 lM) was mixed with various concentrations of heparin,...

Báo cáo khoa học: Oxygen binding and its allosteric control in hemoglobin of the primitive branchiopod crustacean Triops cancriformis pdf

... interval of L was calcu-lated from best-ﬁt values and the standard errors of lT, lR, and L according to the law of error propagation [64].Statistical analysisUnless otherwise stated, data are ... F-statistics predicted the increase in the vari-ance (or the SSR) of a ﬁt that is associated with onestandard deviation of the parameter.AcknowledgementsThe technical assistance of Anny Bang, ... microcrustacean Daphnia magna :a conceptual approach. Adv Exp Med Biol 510, 101–107.26 Pirow R, Ba¨umer C & Paul RJ (2001) Beneﬁts of hae-moglobin in the cladoceran crustacean Daphnia magna.J...

Báo cáo khoa học: DNA binding and partial nucleoid localization of the chloroplast stromal enzyme ferredoxin:sulﬁte reductase pptx

... shift assay A double-stranded 40-mer DNA probe was prepared byannealing the oligonucleotide 5¢-AGTCTAGACTGCAGTTGAGTCCTTGCTAGGACGGATCCCT-3¢ and its com-plementary strand. A 20-mer dsDNA probe was ... 1146–1157.59 Sasaki N, Kuroiwa H, Nishitani C, Takano H,Higashiyama T, Kobayashi T, Shirai Y, Sakai A, Kawano S, Murakami-Murofushi K et al. (2003) Glomis a novel mitochondrial DNA packaging protein ... reductaseKohsuke Sekine1,2, Makoto Fujiwara2, Masato Nakayama3, Toshifumi Takao3, Toshiharu Hase3 and Naoki Sato1,21 Department of Molecular Biology, Faculty of Science, Saitama University,...

Báo cáo khoa học: DNA-binding and transcription characteristics of three cloned sigma factors from mustard (Sinapis alba L.) suggest overlapping and distinct roles in plastid gene expression doc

... 5¢-GAGAAACAAGTGATACGTTGGAGA-3¢ and 5¢-TTTCCTGAATGCAGATGACTCTAT-3¢from AtSig2 and 5¢-GTAGAGATGAACTGGTGAAAAGCA-3¢ and 5¢-AAAACTTGTAACCCCTTGTGTGAT-3¢ from AtSig3, were used for PCR-ampliﬁcationfrom total S. alba DNA. ... sequencetags with sequence similarity to the coding regions of the AtSig2andAtSig3 genes from Arabidopsis thaliana(GenBank accession numbers AC003981 and N97044) Two pairs, 5¢-GAGAAACAAGTGATACGTTGGAGA-3¢ ... the PCR primers 5¢-CACACAAGGGGTTACAAGTTCTCCACG-3¢ and 5¢-ACCAGCCAATTGGTTCCAAAAATCTATCT-3¢ and ligationinto pQE31 (Qiagen). Following transformation of M15,the recombinant proteins were puriﬁed...

Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through speciﬁc protein interactions in the methionine synthase–methionine synthase reductase complex docx

... exogenous aquacob(III)alaminto cob(II)alamin [Yamada K, Gravel RA, TorayaT & Matthews RG(2006) Proc Natl Acad Sci USA 103, 9476–9481]. Here, we report theexpression and puriﬁcation of human methionine ... domain of neuronal nitric oxide synthase) to: (a) stimulate formation of holo-MS from aquacob(III)alamin and theapo-form of MS; and (b) reactivate the inert cob(II)alamin form of MSthat accumulates ... Km of NADPH and the Kact of MSR), AqCbl and dithiothreitolwere replaced by varying concentrations of MSR and NADPH.Anaerobic hMS reactivation assayThe anaerobic hMS assays were performed in a Belle...

Tài liệu Báo cáo khoa học: The antibacterial and antifungal properties of trappin-2 (pre-elaﬁn) do not depend on its protease inhibitory function pptx

... inﬂuenzae,Streptococcus pneumoniae, Branhamella catarrhalis and the pathogenicfungi Aspergillus fumigatus and Candida albicans, in addition to P. aerugin-osa and S. aureus. A similar antimicrobial ... N-terminal domain and is comparable to that of human defensins and human lysozyme.Elaﬁn and trappin-2 are both antimicrobial againstS. aureus and P. aeruginosa [14,15] but not againstE. coli ... been shown to have antibacterial and antifungal properties, whereasrecent data indicate that trappin-2 has antimicrobial activity against Pseu-domonas aeruginosa and Staphylococcus aureus. In the...

Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf

... L, Hamid Q & Elias JA (2004) Acidicmammalian chitinase in asthmatic Th2 inﬂammation and IL-13 pathway activation. Science 304, 1678–1682.4 Kasprzewska A (2003) Plant chitinases ) regulation ... 1084–1092.35 Kawase T, Yokokawa S, Saito A, Fujii T, Nikaidou N,Miyashita K & Watanabe T (2006) Comparison of enzymatic and antifungal properties between family 18 and 19 chitinases from S. ... chitinaseshave catalytic domains that are at least as large asthose of the plant enzymes and that may contain atleast six subsites [26,30]. However, the catalyticdomains of ChiG, ChiC and most other...

Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the speciﬁcity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf

... bond networkaround ArgB263 provide no reasonable explanation of thePA activity with substrates like PhAc-pAB, PhAc-mAB,PhAc-oAB vs. phenylacetyl 4-nitroanilide (PhAc-pNA),NIPAB and iso-NIPAB, ... Distances (A A˚) between polar substrates groups (CO2– and NO2) and the two ArgB263 and ArgA145 residues of penicillin acylase as a result of AM1 optimization of the corresponding substrate ... L.M., Wegman,M .A. , Cao, L. & Janssen, M.H .A. (2001) Biocatalysis and biocatalyst in the synthesis of b-lactam antibiotics. In: Synthesis of b-Lactam Antibiotics. Chemistry, Biocatalysis and Process...

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