Báo cáo khoa học: The periplasmic peptidyl prolylcis–transisomerases PpiD and SurA have partially overlapping substrate specificities doc
Báo cáo khoa học: The periplasmic peptidyl prolylcis–transisomerases PpiD and SurA have partially overlapping substrate specificities doc
... doubledeletion of the ppiD and surA genes did not result inloss of viability.To investigate how these PPIases interact with theirsubstrates and whether they have potentially overlap-ping substrate ... SurA, indicating that the two PPIases might interact with different substrates. Wetherefore propose that, although PpiD and SurA have partially overlapping substrate specificities, they fulfil different ... simultaneous deletion of both ppiD and surA genes was found to confer synthetic lethality, and therefore it was suggested that these PPIases have an overlapping substrate specificity [14]. However,...
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Tài liệu Báo cáo khoa học: The capsid protein of human immunodeficiency virus: intersubunit interactions during virus assembly doc
... conditions;(b) the different shapes of the immature and the matureHIV-1 capsid [32], and of the mature capsids of differentretroviruses; (c) the different structural organization ofCA in the immature and ... membrane, and the remainingdomains forming the innermost protein layers accor-ding to their positions in Gag: MA, CA-NTD,CA-CTD, the spacer peptide SP1, NC, the spacerpeptide SP2 and the C-terminal ... residues in the 310-helix of one monomer and residues in helices 9 and 10 of the other monomer. NoMHR residues are involved. The structural descriptionof the dimerization interface in the isolated...
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Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx
... than 17 A˚apart from the exocyclicmethylene C-atom of the MIO prosthetic group.On the basis of the experimental structures, hypothe-ses on the role of the Tyr110-loop have been put for-ward. ... from the PAL-based models. Merging the MIO portions to the mono-mers and building the ‘Tyr-loop-out’ tetrameric models(PlPALout and SmPALout) was achieved in the same way asdescribed for the ... the biosynthesis of the photoactive yellow proteinchromophore of these bacteria. The two recently discovered aminomutases, the phe-nylalanine 2,3-aminomutase (PAM) involved in taxolbiosynthesis...
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Báo cáo khoa học: The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction docx
... on the dynamics of the cytoskeleton, the former promoting the recruitment of elements of the Rac1 signaling pathway, and the latter regulatingRho activity [47–50]. However, investigating whether the ... the bandswas then determined by densitometry. Plots of the con-centration of MBP-DHPH2 in the pellets versus totalMBP-DHPH2 protein added to the reaction mixtureare reported in Fig. 6B, and ... association completely. Thesedata support the hypothesis that the DHPH2 domainis largely responsible for the interaction between full-length RasGRF1 and microtubules. The DHPH2 module does not...
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Báo cáo khoa học: The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides pot
... single band on t hese gels, as the ODN h ybridizes to the center of the input target RNA. The asterisk indicates the input targetRNA; the arrow indicates the digested product bands. The amount ... levels of eitherRNase H1 or H2. The discrepancy between the in vivo and in vitro measurements could be due to a compartmentaliza-tion of a component in the in vivo system. On the otherhand, we ... distribution over the cell lines. 15PC3 and Mia-PacaII display the highest l evel of the 1.2-kb mRNA, and HEK293 the lowest. The 5.5-kb mRNA species detected by the RNase H2 probe (described by Wu...
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Báo cáo khoa học: The occurrence of riboflavin kinase and FAD synthetase ensures FAD synthesis in tobacco mitochondria and maintenance of cellular redox status docx
... EGFP–AtRibF2[43]. The hypothesis for the localization of FADS(AtRibF1 and AtRibF2) isoforms in mitochondriacannot, moreover, be ruled out on the basis of bioin-formatics (see TAIR). Whether and how ... in the mitochondrial fractionthan in protoplasts (Fig. 1). The mitochondrial and the extramitochondrialamounts of Rf, FMN and FAD in the acid-extractablefractions were measured via HPLC and ... (proto),mitochondria (mt) and plastids (0.05–0.1 mg) the amount of SDH-Fp, detected with a-FAD, and the FUM, PGI and ADH activitieswere measured, as reported in Experimental procedures. The values of the enzymatic...
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Báo cáo khoa học: The viral SV40 T antigen cooperates with dj2 to enhance hsc70 chaperone function docx
... DnaJs lack the cysteinerepeats and type III DnaJs lack both the G ⁄ F region and the cysteine repeats. Type I and Type II proteinsseem to have similar functions and they bind non-native substrates, ... antigen whichincluded the J domain, the Rb-binding domain and the DNA-binding domain, but lacked the ATPase domainof the C-terminus. Given that the ATPase domain of the T antigen has been shown ... bacterial DnaJ and the yeast Ydj1 [10–12]. It ismainly cytosolic and acts as a cochaperone to hsc70assisting the folding of denatured proteins and facilitat-ing the protein import into the mitochondria...
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Báo cáo khoa học: The potent inhibitory activity of histone H1.2 C-terminal fragments on furin doc
... masses and the calculated ones of the recombinantproteins were aligned in the table. (B) The sequence of the synthetic 36- amino acidpeptide around the reactive site (underlined) and the mass ... suggesting that the inhibi-tion was temporary.To identify the reactive site of the inhibitor, the P4fragment was incubated with furin and the reactionwas stopped at the indicated times and analyzed ... by a random coil (negative peak at 196–193 nm), and the contents of the a-helix (estimated from the ellipticity value at 222 nm [24]) are only 1.6, 1 and 0.5% for full-length H1, P4 and the 36-...
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Báo cáo khoa học: The interaction between casein kinase Ia and 14-3-3 is phosphorylation dependent pot
... 14-3-3 g and c isoforms bind most tightly to both the phospho-peptide and the dephosphopeptide and have been identified both in vitro and in vivo as bind-ing most strongly to CKIa (Figs 1–3). The ... Therefore, the difference inphosphorylation dependence of the interactions thatwe have demonstrated in the present study has impor-tant implications for the respective roles of centaurin-a and the ... and 0.1% Triton] were lysed by sonication and clar-ified by centrifugation. The GST fusion protein was removedfrom the lysate using glutathione Sepharose 4B beads (GEHealthcare), and then the...
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Báo cáo khoa học: A new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-jB pathway in skeletal muscle pdf
... each condition. The box embraces the 25th and 75th percentile values. Whiskers extend from each end of the box to the minimum and maximum values in the data.Crosses represent the mean value.Bio-informatics ... 71. Considering the location of the N-terminal extremity of DNter2 on the structure ofCARP and the presence of the potential cleavage sites,we identified the localization of the cleavage site ... full-length or the trun-cated CARP constructs (DNter1–4). The molecular weight of DNter2 matches the lower band detected when CARP is co-expressed withcalpain 3 (37 kDa band; compare the first and the...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Thơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ