... were extracted, and the targetZI3 1A gene was amplified by PCR with primers 5¢-AAATATAAAACGCTAGCGTCGACATGGCGC-3¢ and 5¢-AGCGTAAAGGATGGGGAAAG-3¢. The final ratio of targetcells was determined by ... amount of target strain(BFG2Z18-I3 1A; ZI3 1A –Fc) and an excess amount of nontarget strain (BFG2118; None–Fc). As in the cur-rent system, the other contained a minor amount of sig-nal-amplifiable ... in yeast. J Biochem 147, 87 5–8 84.20 Togawa S, Ishii J, Ishikura A, Tanaka T, Ogino C &Kondo A (2010) Importance of asparagine residues atpositions 13 and 26 on the amino-terminal domain...