Báo cáo khoa học: Metal exchange in metallothioneins – a novel structurally significant Cd5 species in the alpha domain of human metallothionein 1a ppt

... ª 2008 The Authors Journal compilation ª 2008 FEBS 2239 Metal exchange in metallothioneins – a novel structurally significant Cd 5 species in the alpha domain of human metallothionein 1a Kelly ... encompasses the metal- binding cysteines between Cys33 and Cys60 of the a domain of native human metallothionein 1a but also includes amino...

Ngày tải lên: 16/03/2014, 06:20

... do- main and cadmium in the a domain. Therefore, the b domain would regulate zinc and copper homeostasis, whereas the a domain may play a central role in heavy metal detoxification [7]. The loosely ... 12 3–1 34. 40 Pan PK, Hou FY, Cody CW & Huang PC (1994) Sub- stitution of glutamic acids for the conserved lysines in the alpha domain affects metal...

Ngày tải lên: 19/02/2014, 07:20

... were extracted, and the target Z I3 1A gene was amplified by PCR with primers 5¢-AAATA TAAAACGCTAGCGTCGACATGGCGC-3¢ and 5¢-AGC GTAAAGGATGGGGAAAG-3¢. The final ratio of target cells was determined by ... amount of target strain (BFG2Z18-I3 1A; Z I3 1A –Fc) and an excess amount of nontarget strain (BFG2118; None–Fc). As in the cur- rent system, the other contained a minor a...

Ngày tải lên: 05/03/2014, 23:20

... accuracy is badly hurt. In contrast, when searching in a constrained space rather than the raw space, the constrained tagger (beam=5) is 10 times fast as the baseline and the tagging accuracy is even ... compute the morph feature of a word as the set of all of its possible tags, i.e. all tag types that are assigned to the word in training data. Furthermore, we ap...

Ngày tải lên: 07/03/2014, 18:20

... is independent of the differ- entiation status (normal vs. c ancer) of the epithelial c ells in the mammary gland. We also show here that the abolition of hormone- mediated transactivation of a ... Primer pair 1 resulted in a PCR product of 216 bp. Additionally, forward primer 5¢-GAGCCCC AAGAAGAAAGA-3¢ and reverse primer 5¢- CATCCAAAATCTCC TCCA-3¢ were used. Primer p...

Ngày tải lên: 16/03/2014, 18:20

... aid of a parser or a chun- ker. Our resulting system obtains accuracies comparable to the current state -of -the- art at a fraction of the computational cost. 1 Introduction Semantic understanding ... whilst interactive human- machine applications require al- most instant response. Another issue is the cost of producing labeled training data required for statisti- cal mode...

Ngày tải lên: 17/03/2014, 04:20

... TTGCGGTTTAGGTACATCCA KCTD10RP TGTGGCTCTGTGGAACATTT Sp1FP GGACTACCTGGAGTGATGCCTAA Sp1RP CCCATCAACGGTCTGGAACT AP-2aFP CAACGTTACCCTGCTCACATCA Real-time PCR AP- 2a RP CAGGTCGGTGAACTCTTTGCA b-actinFP GCGCGGCTACAGCTTCA b-actinRP ... ChIP AP- 2a ⁄ chipR GAAAAGTCGGAGGACG ChIP Sp1 ⁄ chipF GGACTACCTGGAGTGATGCCTAA ChIP Sp1 ⁄ chipR CCCATCAACGGTCTGGAACT ChIP AP- 2a ⁄ si GCUCCACCUCGAAGUACAATT RNAi Sp1 ⁄...

Ngày tải lên: 30/03/2014, 02:20

... present in the petal margins, whereas the nod- ules in the interior parts of the petals are elongated tubulars [10,12]. In this study, nodules in both the margins and the interior parts of the petals ... accumulation of hypericins, indicating that HpPKS2 may have a role in the initial key reaction step in the biosynthesis of hypericins in H. perforatu...

Ngày tải lên: 18/02/2014, 18:20

... mid-way along the chain. Northern blot analysis showed high level expression of the CYP4x1 RNA in brain and in aorta, and this was confirmed by analysis of the EST database; this showed significant ... RNA for RNAase protection assay. M indicates the 124-base pair marker transcript (M), the full-length probe is indicated by a line, and the protected fragment is indicated...

Ngày tải lên: 19/02/2014, 07:20

... indicate that TM12 plays a key role in the progression of the ATP hydrolytic cycle in ABCB1 and, in particular, in coordinating conformational changes between the NBDs and transmembrane domains. Abbreviations ABC, ... interface. There was no alteration in the extent of labelling by BM in any conformational state examined. In contrast, there was a dramatic reducti...

Ngày tải lên: 06/03/2014, 22:21