Báo cáo khoa học: Arginine-induced conformational change in the c-ring ⁄a-subunit interface of ATP synthase ppt

... result indicates that the hydrophobic interaction at the subunit interface of the dimer increases with PLP binding. Upon binding of PLP to the active site of one b subunit (bA), the side chain of ... bonds between the a and b subunits at their interface in the a 2 b 2 complex. The long loop and the COMM domain constitute the interface of the N and C dom...

Ngày tải lên: 15/03/2014, 11:20

... containing atRE in the presence of 1m M bipyridine (B) and liposomes containing atRE, in the presence of 1 m M bipyridine and 6 mM FeSO 4 (C). The generated retinoids were analyzed by HPLC. The ... contribute to providing chromophore to melanopsin to maintain their light sensitivities. The expression and function of RPE65c in other cell types in the retina remains to...

Ngày tải lên: 14/02/2014, 14:20

... most of the protein components are integral in the membrane, most of these mitochondrial pro- teins behave as if they have a-helical transmembrane domains, rather than b-barrels. These proteins ... protected in the intermembrane space (Fig. 3C). An antibody recognizing the GFP tag at the C-terminus of Om14-GFP shows the electrophoretic mobility of the fusion protein...

Ngày tải lên: 19/02/2014, 07:20

... protein indicates that the structure of the N-terminal domain is maintained, at least in the vicinity of the tryptophan residues. The increase in exposed clustered hydrophobicity observed for the mutants ... Frequency of amino acid residues in the flexible region of the C-terminal extensions of human and murine sHsps. Residues present in the flexible region...

Ngày tải lên: 07/03/2014, 04:20

... The PH domain of TFKs usually binds to phosphatidylino- sitol-3,4,5-trisphosphate, thereby bringing them in close proximity to other membrane-tethered signaling proteins. In SYK, the linker B region ... pro- tein kinase B (PKB)]. In AKT, a cysteine substitution of the corresponding R25 in the pleckstrin homology (PH) domain also results in loss of function [16,17], the...

Ngày tải lên: 14/02/2014, 18:20

... reaction of the luciferin ⁄ luciferase mixture, energy donor (ATP) and oxygen results in the emission of light. The intensity of the bioluminescence was detected using a luminometer, and the mitochondrial ... that these changes occur in an Akt-independent manner. Further studies are therefore required to identify the precise signaling mechanisms that control the metabo...

Ngày tải lên: 18/02/2014, 16:20

... integrin-dependent cell volume sensing and signaling integrates into the overall context of insulin signaling. Similarly, sensing of glutamine-induced hepatocyte swelling by integrins feeds into ... hepatocyte swelling [12,13], insu- lin-induced hepatocyte swelling is registrated by the integrin system, leading to a Src-dependent activation of the p38-type MAPK and thereby inhibit...

Ngày tải lên: 18/02/2014, 16:20

... the densities correspond to the N- and C-terminal helices of the c ring, respectively. The C-terminal helices show a clear handedness, and two of the rings face in the opposite direction in the membrane ... to that of the c ring present in native ATP synthase solubi- lized in the same detergent (Fig. 1). Fig. 1. Isolation and subunit composition of the...

Ngày tải lên: 18/02/2014, 17:20

... Follistatin, Chordin and Noggin [12]. These proteins are antagonists of BMP and other members of the TGF-b family, binding to and inhibit- ing these signaling molecules from binding to their receptors ... rate of Chordin, which may change the preference of site utilization; and (c) promotion of the dissociation of Chordin cysteine-rich (CR)-containing fragments from the...

Ngày tải lên: 19/02/2014, 00:20

... definite descrip- tions and nicknames. 1 Introduction Most of the existing approaches to opinion mining propose algorithms that are independent of the text genre, the topic and the target involved. ... Also interesting is the information contained in the distributions of positive opinions. that there is a large correlation ( The Pearson corr lation coefficient is r...

Ngày tải lên: 20/02/2014, 05:20