Báo cáo khoa học: Interactions of elongation factor EF-P with the Escherichia coli ribosome doc

... posi- tion of the EF-P protein on the Escherichia coli 70S ribosome and its subunits. Binding of labeled EF-P to ribosomes To study the binding site of EF-P on ribosomes, we first ascertained that ribosomes ... deter- mined, as was the area of each peak. The ratio of the density, which is related to the amount of EF-P bound, to the area of the peak...

Ngày tải lên: 16/03/2014, 06:20

... given within parentheses after the UniProtKB ⁄ Swiss-Prot ID. For UniProtKB ⁄ TrEMBL IDs, the accession number forms the first part of the ID. All type 1 PPases are found in the upper part of the ... in the highest yield, and they all were among the amino acids found in the Murchison mete- orite [11]. Both the high content of the ‘very early’ amino acids in these se...

Ngày tải lên: 30/03/2014, 10:20

... A ˚ crystal structure of EF-G bound to the T. thermophilus ribosome showed the FA-binding site for the first time, and revealed the detailed interactions of EF-G with the ribosome [22] (summarized ... Gly617 are in the area of contact with the 1095 and 2473 regions of 23S RNA. The two helices are facing the ribosome, and the four-stranded b-sheet is facin...

Ngày tải lên: 06/03/2014, 22:21

... h after the start of treatment with EGF, indicating that EGF regulated the level of the Bcl-X L protein at the transcriptional level. Next we determined the effect of ADR on the expression of Bcl-X L (Fig. ... did not mediate the antiapoptotic action of EGF in TMK-1 cells. EGF increased the expression of the Bcl-X L protein, an antiapoptotic member of the B...

Ngày tải lên: 19/02/2014, 06:20

... so the nitro- genous group of agmatine must be within 3 A ˚ of N5 of the isoalloxazine ring of the flavin. Agmatine, however, binds poorly to MAO-A, giving only 50% inhibition of the oxidation of ... expected for ligands that alter the spectrum of the enzyme. In order to compare the conformation of all three ligands near the flavin in MAO-A, the docking was rep...

Ngày tải lên: 07/03/2014, 10:20

... at the last base, G to A, abolished the interaction of HIPPI, so the last motif would not interact with HIPPI. The other two motifs might be the target of HIPPI. In the upstream sequence of the Table ... whether HIPPI could interact with the 5¢-AAAGACATG-3¢ () 101 to ) 93) motif present in the putative promoter of the caspase-1 gene. Interactions of HIPPI...

Ngày tải lên: 07/03/2014, 10:20

... changed by any of the four types of CRLPs. Effect of the fatty acid composition of CRLPs on COX-2 mRNA expression The effect of CRLPs of varying fatty acid composition on expression of COX-2, an ... keeping with the reduced level of NF-jB activation following CRLP treatment. There has been little study of the effects of CRLPs on macrophage cytokine synthesis, b...

Ngày tải lên: 23/03/2014, 04:20

... to the next in relation to the vigor of the cell culture at the time. Thus, unless otherwise indicated, for each experiment we show a typical result of one of the multiple occasions that the experiment ... 3B). The specificity of the binding is again confirmed by the competition of unla- belled DR1 probe. The presence of USP in the com- plex was confirmed by a s...

Ngày tải lên: 23/03/2014, 13:20

... showing the amidolytic activity of bound FXIIa in the absence (n) and presence (s, d) of the C1-INH (the slope of the lines is 1/V max ; V max is the maximal activity of the bound FXIIa). The intercept ... binding to the cells by determining the S-2302 amidolytic activity of the bound FXIIa. In the absence of C1-INH the activity increased hyperbolically w...

Ngày tải lên: 23/03/2014, 20:22

... NF-jB activity, the biological significance of the FIH–IjBa interaction was not identified. In the present study, we investi- gated the consequence of the FIH–IjBa interaction in the opposite direction, ... mediated the transcription of the HIF1A gene during lipopolysac- charide stimulation of human monocytes. Recently, the role of NF-jB in HIF-1a mRNA expression was...

Ngày tải lên: 29/03/2014, 23:20