Báo cáo khoa học: Structure–function analysis of the filamentous actin binding domain of the neuronal scaffolding protein spinophilin pot

... at the acidic C-terminus of the protein is not occupied. MS of the nonreduced protein showed a mass of 23 700.0 Da. The difference between the molecular masses of the reduced and nonreduced protein ... first appears in the matrix but is then transferred through the tubule wall at the end of morphogenesis to form spines on the external surface of the inve...

Ngày tải lên: 07/03/2014, 12:20

... studies of the actin binding domain of spinophilin. We demonstrate that the spinophilin actin binding domain is intrinsically unstructured, and that, with increasing C-terminal length, the domain ... of resi- dues 1–221 of spinophilin and F -actin, an apparent shift of actin from the pellet to the supernatant fraction can be observed. H. Schu ¨ ler and...

Ngày tải lên: 16/03/2014, 06:20

... Neo-r9, the median fluorescent intensity (MFI) of 12G5 mAb binding to MT2 cells was 55.56, whereas that of the isotype control was 4.0. In the presence of 2 and 10 lm of Neo-r9, the MFI of the mAb binding ... 2007 The Authors Journal compilation ª 2007 FEBS purpose of the study examining the inhibition of the 12G5 mAb binding to CXCR4 by the several APAC...

Ngày tải lên: 16/03/2014, 06:20

... apparent additivity of the stoichiometries of the complexes ren- dered by the separate moieties of CeMT1 and CeMT2, the summation of their CD spectra did not give rise in any case to spectra close to those of the entire ... terminal one. In the absence of a comprehensive analysis of the metal -binding abilities of CeMT1 and CeMT2, the cur- rently available i...

Ngày tải lên: 07/03/2014, 02:20

... bp intervals. The lighter colour shades depict the bottom half of the nucleosome, and the exploded view below the octamer depicts the arrangement of the histones con- tacting 73 bp of DNA within ... Since then there has been an explo- sion of papers on the multitude of chromatin modifica- tions and the factors that can either create or recognize them. We now have a...

Ngày tải lên: 14/02/2014, 18:20

... C-terminal domain in red. (B) Ribbon representation of the structure of the PSI domain of barley prophytepsin [25] (N-terminal domain, blue; C-terminal domain, red). (C) Model structure of the PSI domain ... linked with each other by three disulfide bridges. A unique feature of the PSI is the swap of the N- and C-terminal portions of the saposin-like domai...

Ngày tải lên: 19/02/2014, 12:20

... sheet with the b12 of the other monomer, and towards the C-terminus, the subsequent residues, Phe182–Lys186, pass through the a2 vicinity of the other monomer. For the FeR dimer, the N-terminal ... of the structure of NADP + -bound FeR revealed that the nicotinamide moiety of NADP + faces the re-side of the isoalloxazine ring, and that the 2¢-P-AMP moiety...

Ngày tải lên: 07/03/2014, 02:20

... [21,53,54]. The lack of a consensus can be attributed, at least in part, to the extrapolation of data from other KH domains to the KH1–KH2 domains of FMRP. The structure of the tandem KH1–KH2 domains of hFMRP ... respectively. The QUA2 domain (labeled) abuts the a2-helix of the KH domain, giving rise to an expanded contact with RNA, with the five nucleotides at...

Ngày tải lên: 07/03/2014, 05:20

... comparable to the hexokinase subunits [24]. One lobe consists of the phosphate1 subdomain and the predicted a-helix I. The other lobe consists of the phos- phate2 and the adenosine subdomains, the predicted ... determination of the K m of GlcNAc, different concentrations of GlcNAc were used in the presence of 10 m M ATP. For determination of the K m of ATP,...

Ngày tải lên: 08/03/2014, 10:20

... depends on the method of produc- tion [27]. The stoichiometry of oleic acid in the complexes probably significantly modifies the mecha- nism of cytotoxicity and the tumor selectivity of the complexes. In ... fully understood. The active group of the matrix, the DEAE group, is posi- tively charged and might therefore bind negative mole- cules. At pH 8.5 (the pH of...

Ngày tải lên: 29/03/2014, 21:20