Báo cáo khoa học: Structure–function relationship of novel X4 HIV-1 entry inhibitors – L- and D-arginine peptide-aminoglycoside conjugates pptx

... 274 (2007) 652 3–6 536 ª 2007 The Authors Journal compilation ª 2007 FEBS Structure–function relationship of novel X4 HIV-1 entry inhibitors – L- and D-arginine peptide-aminoglycoside conjugates Ravi ... anti -HIV-1 and mode of action of neomy-cin and neamine conjugated at speciﬁc sites to arginine 6- and 9-mersd- and l-arginine peptides (APACs). The d-APACs inhibit the infectivity of X4 HIV-1 ... of more potent HIV-1 cell entry inhibitors. AbbreviationsAAC, aminoglycoside-arginine conjugates; ALX40-4C, N-a-acetyl-nona- D-arginine amide; APACs, aminoglycosides poly D- and L-arginine conjugates; ...

Báo cáo khoa học: Structure ⁄function analysis of spinalin, a spine protein of Hydra nematocysts doc

... spinalin peak was often observed at56 0–6 30 mm NaCl. SDS ⁄ PAGE in the absence of areducing agent showed that the spinalin of the ﬁrstpeak migrates as a double band in the gel, with oneband running ... which,ABCDFig. 2. Expression of spinalin in HEK293 cells and puriﬁcation of the recombinant protein. Aliquots of serum-free culture medium of the cells(lanes 1) or of the protein puriﬁed by MonoQ ... Morphology and mor-phodynamics of the stenotele nematocyst of Hydraattenuata Pall (Hydrozoa, Cnidaria). Cell Tissue Res224, 26 9–2 90.2 Holstein T (1981) The morphogenesis of nematocytes inHydra and...

Báo cáo khoa học: Structure–function analysis of the ﬁlamentous actin binding domain of the neuronal scaffolding protein spinophilin pot

... residues 1–1 54, 1–2 21, 1–3 05, 15 4–2 21, 15 4–3 01 and 22 1–3 05 were pro-duced in Escherichia coli and puriﬁed to homogeneity,free of afﬁnity tags used for increased solubility duringexpression and puriﬁcation. ... oneexception: the number of valine residues was doubledin the 1–2 21 and 1–3 05 sequences of spinophilin. Thus,the increasingly structured C-terminal regions of resi-dues 1–2 21 and 1–3 05 of spinophilin ... indicative of F-actin binding, whereas residues 15 4–2 21,15 4–3 05 and 22 1–3 05 of spinophilin do not cosediment withF-actin (arrowheads). (B) Cosedimentation assay of F-actin and resi-dues 1–2 21 of spinophilin...

Tài liệu Báo cáo khoa học: Structure-activity relationships of a-conotoxins targeting neuronal nicotinic acetylcholine receptors ppt

... speciﬁcity and potency, A10 and N11, are shown in pink and labeled. Residues 4–7 and 9–1 0 in the closelyrelated PnIB have also been shown to inﬂu-ence activity [38].Ó FEBS 2004 Structures of neuronally ... in the residue type and in the case of loop 2 in both thetype and number of residues. The N- and C-termini are labeled. (B) On the left is the three-dimensional structure of the 4/6 framework ... – – GIDIRDcCCSNPACRVNNOHVC 4/7 a7, a3b2, a4b2a5, 3, 150 [16] NMR 0.34PIARDPCCSNPVCTVHNPQIC* 4/7 a6b2b3, a3b2a0.95, 74.2 [31] – – AnIBGGCCSHPACAANNQDYC* 4/7 a3b2, a7a0.3, 76 [30] – – AuIAGCCSYPPCFATNSDYC*...

Tài liệu Báo cáo khoa học: parDtoxin–antitoxin system of plasmid R1 – basic contributions, biotechnological applications and relationships with closely-related toxin–antitoxin systems ppt

... 215,46 3–4 68.31 Jensen RB, Grohmann E, Schwab H, Diaz_Orejas R &Gerdes K (1995) Comparison of ccd of F, parDE of RP4, and parD of R1 using a novel conditionalreplication control system of plasmid ... our understanding of impor-tant aspects of the autoregulation of the operon. Inparticular, Tsuchimoto and Ohtsubo [37] described theinteraction of fusion variants of the proteins of the sys-tem ... parD of R1 and the ccd system of F whosetoxins belong to the same superfamily. We often referto the pem system, which is identical to parD of R1 and was identiﬁed in plasmid R100 [20], and to...

Tài liệu Báo cáo khoa học: Glucuronate, the precursor of vitamin C, is directly formed from UDP-glucuronate in liver pptx

... UDP-glucuronosyltransferase(s). Inhibitors of b-glucuronidase and esterases did not affect the formation of glucuronate,arguing against the involvement of a glucuronidation–deglucuronidationcycle. The sensitivity of UDP-glucuronidase ... (1979) A method for the estimation of esterase synthesis and degradation and its application toevaluate the inﬂuence of insulin and glucagon. Eur JBiochem 102, 50 9–5 19.19 Touster O, Aronson NN ... Speciﬁcity of human UDP-glucuronosyltransferases and xenobioticglucuronidation. Life Sci 57, 181 9–1 831.32 Ethell BT, Anderson GD & Burchell B (2003) The effect of valproic acid on drug and steroid...

Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc

... input variables and to explain thebehaviour of the branch-point in terms of the kineticproperties of CGS and TS.Sensitivity analysisIn a ﬁrst analysis, ﬂuxes of cystathionine and threonine(Fig. ... independent of what happens on the cystathionineside. The Phser branch-point combines the divergence of two ﬂuxes (ﬂuxes of cystathionine and threonine) with theconvergence of two ﬂuxes (ﬂuxes of Phser ... [10,1 3–1 5] and inhibited by AMPin vitro [16,17]; (e) Piinhibits the activity of both CGS and TS [12,17]; (f) the enzymatic products cystathionine and threonine do not inhibit the activities of...

Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf

... right-handed aglycon helix wasgreater than that of DDI-1B; (e) the enhancementeffects of DDI-1A and DDI-1B on AAT, CAG and CA repeats are stronger than that of the ATT, CTG and GT strand, which ... thanthose of their complementary strands, ATT and GT(Fig. 1B,C). As such, the enhancement effects of AB CDE FFig. 1. Expansion of the various repeats and the effect of diastereomers DDI-1A and DDI-1B ... (concentrations of 1 0–1 00 lm) of DDI-1A and DDI-1B did not affect DNA slippage (data notshown). Interestingly, there was a similar hierarchy of intensities for the three bands in the (AAT)3⁄ (ATT)5and...

Báo cáo khoa học: "An Empirical Study of Active Learning with Support Vector Machines for Japanese Word Segmentation" pptx

... LanguageProcessing and Very Large Corpora, pages 1 8–2 5.Taku Kudo and Yuji Matsumoto. 2000b. Use of supportvector learning for chunk identiﬁcation. In Proceed-ings of the 4th Conference on CoNLL-2000 and LLL-2000, ... of Active LearningWe use pool-based active learning (Lewis and Gale,1994). SVMs are used here instead of probabilisticclassiﬁers used by Lewis and Gale. Figure 1 showsan algorithm of pool-based ... current increment of support vectors isless than half of the average increment.Table 2: Accuracy at Different Labeled Data Sizeswith Random Sampling #of Sen-tences# of Ex-amples #of BinaryFeaturesAccuracy(%)21...

Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf

... recruitment of MOF to recently transcribed regions, especially at the3¢ ends of genes where H3-K36me3 is enriched [128].In mammalian cells MOF also exists as part of a sepa-rate MOF–MSLv1 complex ... withbinding of both MOF and Tip60 (equivalent to Esa1in yeast NuA4) [124,130]. However, in human cellsdepletion of MOF, but not Tip60, results in reducedglobal levels of AcH4-K16 and defective ... embryos, MOF is essentialfor AcH4-K16, and loss of MOF results in embryonic-lethal chromatin condensation [13 2–1 34]. In yeast,acetylation of H4-K16 is also required to suppress thespread of heterochromatin,...

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