Báo cáo khoa học: Role of the structural domains in the functional properties of pancreatic lipase-related protein 2 pot

... levels [13 ,23 ,28 ,30–33]. The genetic evidence for the role of AMPK in the hypothalamus is less clear because the loss of the AMPKa2 subunit in specific hypothalamic cell types resulted in the opposite ... promoting an increase in proopiomelanocortin and cocaine and amphetamine regulated transcript. Taken together, these findings provide a compelling argument for th...

Ngày tải lên: 14/02/2014, 22:20

... concentrations. The d 2 -crystallin in lens was recruited during evolution from argininosuccinate lyase, an enzyme involved in arginine biosynthesis derived from the urea cycle. These two proteins shared ... hydrophobic surfaces of the protein were assayed by incubating the GdnHCl-denatured protein in the dark with ANS (50 l M )for4hat25°C. The fluorescence emission s...

Ngày tải lên: 08/03/2014, 08:20

... pivotal role of the UPR is to maintain ER home- ostasis. Therefore, the presence of mutated proteins that are unable to fold into their native conformation in the ER induces the UPR in an effort ... peptide in the 24 -kDa a-zein and the 19-kDa a-zein endosperm storage proteins, respect- ively. These mutant proteins are translocated into the lumen of the ER, but...

Ngày tải lên: 16/03/2014, 11:20

... compilation ª 20 08 FEBS 54 Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- dye binding. Anal Biochem 72, 24 8 25 4. 55 ... strains to obtain the new strains reported in Table 1. Genomic HA-tagging of SDH1 Three consecutive copies of the HA epitope were fused to the 3¢-end of...

Ngày tải lên: 23/03/2014, 07:20

... the C-domain. The dis- tance of the S1003A residue to the active-site histidine of the C-domain is 16 A ˚ , making it within the reach of the missing Ppant arm (capable of reaching 20 A ˚ dis- tance ... these changes with intact proteins in solution. Furthermore, a close inspection of the results showed differences between the apo -protein and the holo-pro- t...

Ngày tải lên: 29/03/2014, 08:20

... 129 1 m 128 4– 128 8 127 9 m 128 1 m 127 3– 127 7 CH 3 bend, CH bend 125 8 w 125 3 m 125 2– 125 7 mCN(IV), mC 7 C 17 , d defs 123 6 m 120 6 sh 123 5– 123 7 dC m H(d), mC a C b (II), CH 2 bend, CH bend1194 sh 120 9– 121 2 1170 ... known as the EF hand [16]. Proteins containing the EF hand are divided into two classes according to their functions: signaling and buffering ⁄ transport pr...

Ngày tải lên: 30/03/2014, 02:20

... Kuwajima K (20 00) Role of the molten globule state in protein folding. Adv Protein Chem 53, 20 9 28 2. 31 Creighton TE (1997) How important is the molten glob- ule for correct protein folding? Trends ... for input data. The urea-induced unfolding curves were obtained by plotting the ellipticity at 22 2 nm against urea concentration. To estimate the urea concentration...

Ngày tải lên: 30/03/2014, 16:20

... of A 228 6, T 228 7 and T 228 8, and the G 229 1T transversion. These four mutations caused three amino acid changes, namely K762N, F763M and the R764D deletion. The rest of the protein remained unchanged. As ... such stress conditions in the absence of Pdr12p. Moreover, recent data indicate the involvement of Pdr12p in the export of by-products of amino-acid c...

Ngày tải lên: 19/02/2014, 00:20

... defining the set of items that have ever been the actor in a flight. Their definition is solely within NIKL using the QUA link [14], which is exactly the set of fillers of a slot. While having ... all of the definitions of approximately 20 0 items from the base vocabulary using the defini. tJons of those vocabulary items themselves in the dictionary. In...

Ngày tải lên: 21/02/2014, 20:20

... meaning of the basic expressions (basic meanings). 2. the meaning of the rules (rule meanings). In Montague Grammar these meanings are ex- pressed in intensional logic. In the Rosetta system the ... grammar defines the domain T of S-trees in the same way as for free M-grammars. The set TM of well-formed S-trees is defined by spec- ifying: 1. a set B of...

Ngày tải lên: 22/02/2014, 10:20