Báo cáo khoa học: Phosphorylation modulates the local conformation and self-aggregation ability of a peptide from the fourth tau microtubule-binding repeat pdf
Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx
... or SYK themselves, is capable of transforming NIH-3T3 cells [70]. In addition, we and others have demonstrated that the activation and plasma membrane localization of the fusion construct are dependent ... key tyrosines that are subject to auto- and ⁄ or transphosph- orylation, and that mediate interaction with Vav, c-Cbl and the p8 5a subunit of phosphatidylinositol 3-...
Ngày tải lên: 14/02/2014, 18:20
Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf
... centrifugation through an oil cushion and dissolved prior to determination of the amount of dyes associated with them. The advantages of such an assay are that: (a) it is quantitative; (b) it is not affected ... they are hydrophobic and negatively charged. A comparison of rhodamine uptake, in the presence and the absence of uncouplers, allows the estimation of t...
Ngày tải lên: 18/02/2014, 13:20
Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx
... significant advantage that enthalpy changes can be monitored directly and data obtained in a model-free fashion [36,37]. Disadvantages of calo- rimetry are the high concentrations and amounts of material ... reports documenting that the lengths of the pre- and post- transition baselines selected and used in data analysis directly affect the values of the thermodynam...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: "Phrase Table Training For Precision and Recall: What Makes a Good Phrase and a Good Phrase Pair?" doc
... can put as many features as possible together under the log-linear model to eval- uate the quality of a phrase and a phase pair. The phrase table extracting procedure is trainable and can be optimized ... used to evaluate the quality of a phrase and a phrase pair. Ideally, a perfect combina- tion of feature functions divides the correct and in- correct candid...
Ngày tải lên: 08/03/2014, 01:20
Báo cáo khoa học: Phosphorylation of the Saccharomyces cerevisiae Grx4p glutaredoxin by the Bud32p kinase unveils a novel signaling pathway involving Sch9p, a yeast member of the Akt / PKB subfamily pot
... exponential phase, and then incubated for 30 min in galac- tose medium to activate the GAL regulon. Total mRNAs were extracted and subjected to standard northern blot analysis. GAL1 mRNA, and ACT1 ... the S13 4A- S ⁄ S13 4A- AS, S13 3A- S ⁄ S13 3A- AS, S13 3A- S13 4A- S ⁄ S13 3A- S13 4A- AS and S134D-S ⁄ S134D-AS pairs of primers (Table S2). Transformation of yeast cells with...
Ngày tải lên: 07/03/2014, 04:20
Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
... -ATC ATC TCC ATC GAC TAC TCC CTG-3¢, the antisense primer 5¢-AAG AAT TCT AGA TTA ATG GTG ATG ATG GTG ATG ATG GTG TGG GGT CAG CGG TGC AGC AGG GGG GGT-3¢ (XbaI sites underlined; His-tag in italic) ... by bis-ANS was decreased upon phosphorylation. A possible explana- tion for this apparent discrepancy could be that PKA phosphorylation induces a conformational change that increases the...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx
... 3¢-GG AACCACGAAAGTAG CAATC AGA ACACTA AAACC A GGTACAATGATGC-5 ¢ . All vector inserts were sequenced prior to use. CDK4 pY17 antibody generation Murex lop-eared rabbits were injected with a phosphopep- tide ... 537 of C-YES was mutated from TAC (Tyr) to TTC (Phe) with the forward primer: 3¢-GTCACAGAGCCACAGTTCCAG CCAGGA-5¢. Codon 305 of C-YES was mutated from AAA (Lys) to AGA (Arg...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt
... 5¢-TCATGCTGGTTGAGACTG GA-3¢ and 5¢-CCAGGTGTGGAGCAAGGCAG-3¢) and a 938 bp fragment of promoter 3 (primers, 5¢-CTCACAG CAGCCAGTAAGTG-3¢ and 5¢-ACTCACAGGCTCTGG GGCTG-3¢) were amplified using a Taq polymerase ... 5¢-GGACTCAAGAGAGGAACCTG-3¢; F1b, 5¢-CT GCCTTGCTCCACACCTG-3¢; F1c, 5¢-CTGCCGTCTCC GCCGCCACT-3¢; FU, 5¢-TCAAGCCCCGCTACATAG TT-3¢; and R3, 5¢-AGGAACCAAACACCAAGTGG-3¢ (Fig. 2A)...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... from cleavage of the core residue Gal (Fig. 3A) , and by 0,3 A 5 at m/z 1111. The C 2a ion at m/z 528 shows that one Fuc is at the subterminal GlcNAc, and the characteristic D 1a- 2a ion [13] at ... fragment ions from both branches, and as no additional fragment ions are apparent, it can be deduced that the disaccharide sequence on the 3-branch shares the same...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Long-distance interactions between enhancers and promoters The case of the Abd-B domain of the Drosophila bithorax complex pdf
... both iab-5 and iab-6 are active, and iab-7 and iab-8 are silenced, but only iab-6 drives the expression of Abd-B. Similarly, although three different Abd-B cis-regulatory domains are active in A7 , the ... of these sequences are derived from the Mcp [11], and the Fab-7 [12] regions. Both contain a boundary and at least one PRE, and are able to medi- ate long-dista...
Ngày tải lên: 20/02/2014, 01:20