Báo cáo khoa học: Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice doc
Báo cáo khoa học: Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice doc
... clearance of soluble CD69 receptors in the blood- stream of C57BL ⁄ 6 mice. (A) The 125 I-radiolabeled recombinant pro- teins were injected into the tail vein of the mice and the radioactivity in individual ... 2008 The Authors Journal compilation ª 2008 FEBS Soluble recombinant CD69 receptors optimized to have an exceptional physical...
Ngày tải lên: 16/03/2014, 04:20
Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane affinity and hyper-anticoagulant activity in mouse plasma pptx
... concentration in the membrane results in an increase in the number of binding sites (compare Fig. 3D and 3E) and also in an average site with increased affinity. If Gla proteins were to bind exclusively to ... mem- brane binding affinity enhancement by minor increases in the overall on-rate and ⁄ or minor decreases in the overall off-rate. The results obtai...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
... available: Fig. S1. Amino acid alignment of linker region and ligand-binding domain (LBD) of ecdysone receptors. Fig. S2. Amino acid alignment of linker region and ligand-binding domain (LBD) of ultraspiracle ... images. The pixel intensity of the band signal was determined for the defined band area and adjusted relative to one of the signals, as designated, to...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Neuropeptide Y-family receptors Y6and Y7in chicken Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region docx
... clone and gave the remaining part of the coding region. The coding part of the Y 6 gene is contained within one exon and encodes a protein of 374 amino acids displaying the characteristics of other ... several other primates [32,34,36], pig [37] and guinea-pig [38], and it has been lost in rat [39]. On the other hand, the gene has an intact open reading fra...
Ngày tải lên: 19/02/2014, 07:20
Báo cáo khoa học: Human recombinant prolidase from eukaryotic and prokaryotic sources Expression, purification, characterization and long-term stability studies pptx
... vector pET16b containing an N-terminal His tag and the sequence for the Factor Xa cleavage site in- frame with the human prolidase sequence under the control of the T7 promoter. Human recombinant ... 55% of the initial activity and the untagged prolidase 66% (Fig. 5B). The addition of MnCl 2 and GSH showed the most promising effects in stabilizing and ac...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: "Veterinary decision making in relation to metritis - a qualitative approach to understand the background for variation and bias in veterinary medical records"
... understanding with regard to decision levels Based on analysis of the veterinarians' perceptions of how they wished to use the metritis score in their practice and on dialogue with the farmer and ... scores and veterinarians' decision making connected to metritis treat- ment and potential links to data quality. This understand- ing provides insight into p...
Ngày tải lên: 25/10/2012, 10:45
Báo cáo khoa học: "Medical emergency teams: deciphering clues to crises in hospitals"
... and the incidence of crisis recognition in hospitals. Their review of over 2000 events revealed an increase in events at certain times of the day, notably near nursing handoffs and physician rounding. ... patients who are in crisis, and then respond to them swiftly and effectively to prevent unnecessary deaths. In 1994, Franklin and Mathew [1] recognized that...
Ngày tải lên: 25/10/2012, 10:45
Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... ligands: the five same ligands in the Mn-reconstituted enzyme and an additional water oxy- gen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed an equatorial ... had an independent metal-binding site at the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal do...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf
... SJS259 and SJS260, and the creation of the ARE II ⁄ ARE IV double mutant used primers SJS261 and SJS262. The mutagenesis of ARE I and III introduced HindIII restriction sites and so the creation of ... was analysed by RNase protection assay or by northern analysis (10 lgof total RNA) using the intron spanning 32 P-labelled globin and GAPDH probes described above....
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
... binding. Point mutations disrupting the RNA-binding capacity of any of the two RRMs led to the nuclear sequestration of the reporter protein, confirming the inability of such mutants to exit the ... in the presence of RNAse. Interestingly, most of the identi- fied proteins are known to be involved in RNA metab- olism and belong to three large families...
Ngày tải lên: 16/02/2014, 15:20