Báo cáo khoa học: Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice doc
... clearance of soluble CD69 receptors in the blood-
stream of C57BL ⁄ 6 mice. (A) The
125
I-radiolabeled recombinant pro-
teins were injected into the tail vein of the mice and the
radioactivity in individual ... 2008 The Authors Journal compilation ª 2008 FEBS
Soluble recombinant CD69 receptors optimized to have
an exceptional physical...
... concentration in
the membrane results in an increase in the number of
binding sites (compare Fig. 3D and 3E) and also in an
average site with increased affinity. If Gla proteins
were to bind exclusively to ... mem-
brane binding affinity enhancement by minor increases
in the overall on-rate and ⁄ or minor decreases in the
overall off-rate.
The results obtai...
... available:
Fig. S1. Amino acid alignment of linker region and
ligand-binding domain (LBD) of ecdysone receptors.
Fig. S2. Amino acid alignment of linker region and
ligand-binding domain (LBD) of ultraspiracle ... images. The pixel intensity of the band
signal was determined for the defined band area and adjusted
relative to one of the signals, as designated, to...
... clone and gave the
remaining part of the coding region. The coding part
of the Y
6
gene is contained within one exon and
encodes a protein of 374 amino acids displaying the
characteristics of other ... several
other primates [32,34,36], pig [37] and guinea-pig [38],
and it has been lost in rat [39]. On the other hand,
the gene has an intact open reading fra...
... vector pET16b containing an N-terminal His tag and the sequence
for the Factor Xa cleavage site in- frame with the human prolidase sequence under the control of the T7 promoter.
Human recombinant ... 55% of the initial activity and the
untagged prolidase 66% (Fig. 5B).
The addition of MnCl
2
and GSH showed the most
promising effects in stabilizing and ac...
... understanding with regard to decision levels
Based on analysis of the veterinarians' perceptions of how
they wished to use the metritis score in their practice and
on dialogue with the farmer and ... scores and
veterinarians' decision making connected to metritis treat-
ment and potential links to data quality. This understand-
ing provides insight into p...
... and
the incidence of crisis recognition in hospitals. Their review of
over 2000 events revealed an increase in events at certain
times of the day, notably near nursing handoffs and physician
rounding. ... patients who are in crisis, and then respond to
them swiftly and effectively to prevent unnecessary deaths.
In 1994, Franklin and Mathew [1] recognized that...
... ligands: the five same ligands in the
Mn-reconstituted enzyme and an additional water oxy-
gen, which, together with the OD2 of Asp165, the
NE2 of His79 and the NE2 of His169, formed an
equatorial ... had an independent metal-binding site
at the interface of the two domains, which consists
of four side chains: two (His31 and His79) from the
N-terminal do...
... SJS259 and SJS260, and the creation
of the ARE II ⁄ ARE IV double mutant used primers SJS261
and SJS262. The mutagenesis of ARE I and III introduced
HindIII restriction sites and so the creation of ... was analysed by
RNase protection assay or by northern analysis (10 lgof
total RNA) using the intron spanning
32
P-labelled globin
and GAPDH probes described above....
... binding. Point
mutations disrupting the RNA-binding capacity of any
of the two RRMs led to the nuclear sequestration of
the reporter protein, confirming the inability of such
mutants to exit the ... in the
presence of RNAse. Interestingly, most of the identi-
fied proteins are known to be involved in RNA metab-
olism and belong to three large families...