Báo cáo khoa học: Fully active QAE isoform confers thermal hysteresis activity on a defective SP isoform of type III antifreeze protein docx

... Fully active QAE isoform confers thermal hysteresis activity on a defective SP isoform of type III antifreeze protein Manabu Takamichi 1,2 , Yoshiyuki Nishimiya 1 , Ai Miura 1 and Sakae Tsuda 1,2 1 ... that the ice growth inhibitory activity of an antifreeze protein isoform lacking the active component is restored by the addition of a minute...

Ngày tải lên: 16/03/2014, 04:20

... 88, 12–19. 19 Ohtani N, Yanagawa H, Tomita M & Itaya M (2004) Identification of the first archaeal type 1 RNase H gene from Halobacterium sp. NRC-1: archaeal RNase HI can cleave an RNA-DNA junction. Biochem ... Type 1 RNase H [19,20]. Archaeal Type 1 RNase H can cleave an RNA–DNA junction (a junction between the 3¢ side of RNA and 5¢ side of DNA) of an Okazaki fragment-like...

Ngày tải lên: 19/02/2014, 18:20

... Invitrogen (Carlsbad, CA, USA). [ 14 C]AA was purchased from Amersham (Piscataway, NJ, USA). Goat anti-(rabbit IgG)–fluorescein isothiocyanate (FITC) conjugate, saponin, SLO, Triton X-100 and triethylenedi- amine ... COX-2 has an inducible nature, has a lower capacity to be stably expressed, and may also lead to numerous pathological processes, such as cancers and inflammation. Given the natu...

Ngày tải lên: 30/03/2014, 02:20

... regulation of proliferation and sur- vival), Janus-activated kinase 2, signal transducer and activator of transcription 3 (which is recognized as an oncogene implicated in many cancers) and extracellu- lar-signal ... nuclear localization in addition to its localization in the cytoplasm and was implicated in a transcription control role. In gene array assays, CgB induced or suppressed tr...

Ngày tải lên: 07/03/2014, 05:20

... 1B, left panel). SDS/PAGE analysis r evealed a single band displaying an ap parent m olecular weight o f 85 kDa, which was quantified based on known concentrations of BSA run on neighboring lanes. ... activity was normalized based on protein concentrations. FACS analysis and cytokine displacement BAF GLC and SK-N-GP cells were incubated in the presence of increasing concentrati...

Ngày tải lên: 08/03/2014, 10:20

... mutation had an influence on the catalytic activity of RPTPa-D2 in vitro by using glutathione S-transferase (GST)-fusion proteins and pNPP as a substrate. The phosphatase activity of D2-R554H was ... indi- cate that the catalytic activity of RPTPa-D2 plays an important role in Src activation. To confirm that the catalytic activity of RPTPa-D2 is required for Src binding and...

Ngày tải lên: 15/03/2014, 10:20

... 5¢-AGCACGTTAATCAATTGACTACGGTTGCATTAGCGC-3¢ Vps4 Ter2 F 5¢-TTAAAAGAACCAGATTAGTCAATTGATTAACGTGCT-3¢ Vps4 Ter2 R 5¢-AGCACGTTAATCAATTGACTAATCTGGTTCTTTTAA-3¢ Vps4 RDE F 5¢-AAGCAAGAACAGTTCACTGCAGCTTTTGGTCAAGCAGGTAACTAGTCAATTGAT-3¢ Vps4 ... 5¢-AAGCAAGAACAGTTCACTGCAGCTTTTGGTCAAGCAGGTAACTAGTCAATTGAT-3¢ Vps4 RDE R 5¢-ATCAATTGACTAGTTACCTGCTTGACCAAAAGCTGCAGTGAACTGTTCTTGCTT-3¢ Vps4 RKI F 5¢-GCGCTAATGCAACC...

Ngày tải lên: 16/03/2014, 13:20

... it participates, without specifying their types in advance. We will generate a concept class and a variety of natural binary relations involving that class. An advantage of our method is that ... domains we considered indicate that, taken as an aggregate, the relations that are gen- erated for a given domain paint a rather clear picture of the range of information pertinent to...

Ngày tải lên: 23/03/2014, 18:20

... other reactions Figure 1: Aspects for TAC’s guided summarization task, category 2: Attacks 3 Fully Abstractive Approach Guided summarization categories and aspects define an information need, and ... a methodology that relies on Informa- tion Extraction and Natural Language Generation, and discuss our early results. 2 Guided Summarization The stated goal of the guided summarization tas...

Ngày tải lên: 30/03/2014, 17:20

... Class-Based Construction of a Verb Lexicon. AAAI ’00. Anna Korhonen, 2002. Subcategorization Acquisition. Ph.D. thesis, University of Cambridge. Hang Li and Naoki Abe, 1998. Generalizing Case Frames ... allowable frames for each type. Consequently, the common evaluation focuses on the quality of the allowable frames acquired for each verb type, and not on the classification of...

Ngày tải lên: 30/03/2014, 21:20