Báo cáo khoa học: Novel diadenosine polyphosphate analogs with oxymethylene bridges replacing oxygen in the polyphosphate chain pdf

... Novel diadenosine polyphosphate analogs with oxymethylene bridges replacing oxygen in the polyphosphate chain Potential substrates and/or inhibitors of Ap4A hydrolasesAndrzej ... tounderstand their biological functions has been through the use of NpnN¢ analogs. Here, we present the results of studies using novel diadenosine polyphosphate analogs, with an oxymethylene group replacing ... in the analogs with respect to their adenosine moieties.Details of the syntheses will be published elsewhere, andwe present, in the Supporting information, only the keysteps leading to the formation...

Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf

... enrichment in iNOS activitywithin the particulate fraction of epithelial cells.Apical distribution of iNOS in intestinal epithelialcellsTo get more insight into the localization of iNOS in intestinal ... of iNOS to apical domains of intestinal epithe-lial cells.Particulate fraction association of iNOS in vivo In order to determine the distribution of iNOS in intestinal epithelial cells in vivo, ... the C-termi-nus of iNOS promotes in vitro interactions with the PDZ protein EBP50 [13]. Interestingly, EBP50 has dif-ferent binding partners including ezrin that can beanchored to the actin...

Tài liệu Báo cáo khoa học: "Arabic Tokenization, Part-of-Speech Tagging and Morphological Disambiguation in One Fell Swoop" pdf

... Proper Nounresulting from missing entries in the Buckwalter’slexicon. The remaining case was a failure in the conversion process relating to the handling of bro-ken plurals at the lexeme level. ... at all. In Arabicnouns, arguably the gender feature is the more inter-esting distinction (rather than the number feature) asverbs in Arabic always agree with their nominal sub-jects in gender. ... canexpress these features. We discuss the training anddecoding of these classiﬁers in Section 5.Third, we choose among the analyses returned by the morphological analyzer by using the output ofthe...

Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt

... two termini of the inserted Ca2+-binding sites in the model structure of the EF-hand of STIM1 is within15 A˚. Accordingly, a total of six glycine linkers is sufﬁ-cient to enable the grafted ... Ca2+-coordinating residues in positions 1 and 3 of the EF-hand motif signiﬁcantlycompromised the metal-binding capability of the engi-neered protein, indicating that the metal binding ofCD2.STIM1.EF ... protein was graduallyadded to the initial mixture. The ﬂuorescence intensity wasnormalized by subtracting the contribution of the baselineslope using logarithmic ﬁtting. The Tb3+-binding...

Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt

... throughout the dynamics in DDwtleading to an uninterrupted stacking at the steps ﬂank-ing the mismatch. The difference in the A C mismatchhydrogen bond may therefore be attributed to the signi-ﬁcant ... absence.These, in turn, bring about dynamic water interactions in the vicinity of the mismatch. Enthalpy loss and the disproportionate entropy gain associ-ated with these are implicated as the factors ... factors contributing to the increase in free energy and diminished stability of PNAÆDNA duplex with the A Cmismatch. Absence of these in the isosequential DNA duplex, notwith-standing the A C mismatch,...

Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

... PtdIns(5)P,PtdIns(3,4)P2and PtdIns(3,5)P2 with the strongestbinding to PtdIns(5)P (Fig. 1). The ORD domainshowed a very similar lipid binding pattern as the full-length protein. While the ... staining in- creased, and ﬁnally punctate staining was almost lost with predominant vacuolar staining at 30 min. Com-pared with wildtype, no delay of FM4-64 transport wasobserved in osh6D. In ... Asshown in Fig. 7, at 0 min of chase, p1 CPY was the predominant form. The p2 form was seen at the 5 mintime point. At a later time (10 min), mature CPY wasobserved together with some remaining...

Báo cáo khoa học: "Combining Acoustic and Pragmatic Features to Predict Recognition Performance in Spoken Dialogue Systems" pdf

... feature indicating the numberof indeﬁnite NPs that can be uniquely resolved in the Information State (#uniqueIndeﬁnites, e.g. “atower” where there is only one tower in the do-main). We include these ... TiMBL with all possible parameter set-tings on the generated training and test setsand store the best performing settings.4. Classify the left-out hypotheses with the recorded parameter settings.5. ... settings (the other results are in- cluded for comparison). The individual rows show the performance when different combinations offeature groups are used for training. The results for the three-way...

Báo cáo khoa học: "Evaluating language understanding accuracy with respect to objective outcomes in a dialogue system" doc

... Compar-ing linguistic features for modeling learning in com-puter tutoring. In Proceedings of the 2007 confer-ence on Artiﬁcial Intelligence in Education: Build-ing Technology Rich Learning Contexts ... use them for predicting the learninggain that a student could achieve when interacting with a new version of the interpretation compo-nent for the system, not yet tested with users. Wecan then ... something correct, but incomplete, with some parts of the expected answer missingcontradictory The student’s answer contains something incorrect or contradicting the expected answer, rather than...

Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf

... side -chain of methi-onine decreases in these two enzymes both the KmforNMP and the kcatcompared with the parent molecules[16,18]. In conclusion, the amino acids interacting with the phosphate ... to the N3atom of cytosine. As the main chain carbonyl ofD129, a H-bond acceptor, interacts with the terminal oxygen of S36 side chain, the latter can only behaveas a H-bond acceptor with the ... domain, is conserved in NMP kinases. It isused as a rigid platform around which the short a-heli-cal LID domain, situated in the C-terminal moiety,and the NMP-binding (NMPbind) domain move in...

Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the speciﬁcity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf

... its S1¢ binding subsite. Substrate bindinginduces conformational changes involving the ArgA145-PheA146 fragment of the protein chain, acting as a ﬂap, ﬁrstopening and then closing the groove ... follows: Ne with the O atom of the main chain CO group of the TrpB240;Ng1shares H bonding with O atom of the COgroup of LeuB387 together with O atom ofc-OH of SerB386; Ng2is in H bonding with Od1of ... X-raycoordinates of amino acid residues in penicillinacylase [19] and AM1 docking calculations. The three N atoms of the side chain d-guanidinogroup of this residue are involved in H-bonding as...

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