Báo cáo khoa học: Pathways involved in testicular germ cell apoptosis induced by H2O2 in vitro doc

... to induce apoptosis in testicular germ cells in vitro. Following 1 h of treatment with 10 lm H 2 O 2 , a 10-fold rise in the percentage of apoptotic cells was observed. Induction of germ cell apoptosis ... to induce apoptosis in testicular Leydig cells in vitro [11]. Germ cells constitute the major inner seminiferous tubular cell mass and the role of H 2 O 2 a...

Ngày tải lên: 16/03/2014, 04:20

... e in vivo. Properties of binding of DmPCNA2 and DmPCNA1 to chromatin damaged by various mutagens As described earlier, PCNA is involved in DNA repair [3]. In humans, the amount of PCNA binding ... of cellular proteins S2 cells were exposed to MMS or mitomycin C for 1 h or to H 2 O 2 for 15 min. The cells were then washed once and incubated prior to sampling. UV-irradiated S2 cells w...

Ngày tải lên: 23/03/2014, 10:20

... signaling pathways in oridonin -induced L929 cell apoptosis. Moreover, oridonin -induced autophagy can block apoptosis by up-regulating the p38-NF-jB sur- vival signal pathway. These inspiring findings ... autophagy up-regulated p38 activation in oridonin -induced L929 cells. Interestingly, we found that inhibition of p38 decreased oridonin -induced NF-jB activation, indicat-...

Ngày tải lên: 18/02/2014, 13:20

... recombinant protein. Raising the cellular redox potential by increasing aeration levels is expected to reduce the level of cysteine and increase the level of cystine in the cell, thus reducing ... characterization of xan- thine dehydrogenase in a baculovirus-insect cell system. In Flavins and Flavoproteins 1993. Proceedings of the 11th International Symposium on Flavins and Flavopr...

Ngày tải lên: 16/03/2014, 23:20

... implemented in the two other remaining units. Approval of the ethics committee was obtained; informed consent was waived. A surgical ICU-independent clinical pharmacist with experi- ence in medication ... modified, since this classification is ade- quate for administration errors, but not entirely for prescription errors. An independent panel, consisting of one clinical pharmacist, not i...

Ngày tải lên: 25/10/2012, 10:39

... cadherin–catenin complexes The cytoplasmic domain of cadherin interacts with F-actin via b-catenin and a-catenin; b-catenin binds to cadherin and a-catenin, which in turn interacts with F-actin (Fig. ... increases in cell cell adhesion. (A) Simultaneous increases in intracellular [Ca 2+ ] in two adherent cells lead to disrup- tion of cell cell adhesion through disassem- bly of cad...

Ngày tải lên: 16/02/2014, 09:20

... in the mitochondrial apoptotic response induced by the toxin remains to be determined. Finally, we assign an important role to the endosomal acidic cathep- sins B and D in increasing the in vitro ... other cell types. In vivo [36] and in vitro [37] studies have shown that the normal airway epithelium is highly resistant to P. aeruginosa -induced apoptosis. Moreover, in...

Ngày tải lên: 18/02/2014, 04:20

... structure of the cobalamin-binding domain Homocysteine Methionine Co Methylcob(III)alamin Cob(I)alamin Primary turnover cycle H CH 3 H 4 folate AdoHyc Cob(I)alamin Cob(II)alamin H 4 folate Co NADPH A doMet e – FAD FMN NADPH NADP + e – Reactivation Co Fig. ... Pichia was adapted from the Invitrogen protocol (Pichia Fermenta- tion Growth Guidelines; Invitrogen). Expression of recom- binant hMS...

Ngày tải lên: 18/02/2014, 08:20

... (made in rat) and gp-anti-dW4 (produced in guinea pig), two antisera discriminating CHH isomers (gp-anti-pQl made in guinea pig and rb-anti- pQd in rabbit) [38] and an antiserum raised against ... coloration for D-cells: D-CHH cells (red, thin arrow), D-VIH cells (green, short arrow) and D-cells producing both D-isomers (orange, long arrows). (G) Sinus gland axonal arborizations conta...

Ngày tải lên: 18/02/2014, 11:20

... structure determination by NMR was performed using a 50 mL incubation of 50 lm vitamin D3 with 2 lm P450scc in 0.45% cyclodextrin, with the product being purified by TLC [4], and then by gradi- ent ... 20-hydroxyvitamin D3 and 20,23-dihydroxyvitamin D3 by P450scc Incubation of 20-hydroxyvitamin D3 in cyclodextrin with P450scc resulted in the formation of 20,23-di- hydroxyvitamin...

Ngày tải lên: 18/02/2014, 17:20