Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx

... A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low K m for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme Joana Relat, ... 0 to 15 lm for P50H, H50P, P128H, H128P, PigE17DCPT1B and HumanD17ECPT1B, and from 0 to 500 lm for D 18 PigCPT1B and D28PigCPT1B)....

Ngày tải lên: 16/03/2014, 04:20

... knock-down of ZNHIT -1 The oligonucleotides encoding the ZNHIT -1 siRNA were 5¢- GATCCGAGACTGCCTCAGTTTGATTCAAGAGATCA AACTGAGGCAGTCTCTTTTTT-3¢ and 5¢-AGCTTAAA AAAGAGACTGCCTCAGTTTGATCTCTTGAATCAAA CTGAGGCAGTCTCG-3¢. ... enzyme [10 ], a- fetoprotein [11 ] and rat apoAI [12 ] genes. Rev-erba is also involved in cir- cadian timing in brain and liver tissue, and regulates Bmal1...

Ngày tải lên: 07/03/2014, 05:20

... 5¢-AAGTAGGCGGAGTATCGAAC-3¢ (sense) and 5¢-GAAAAAACGCGATCCTACTT-3¢ (antisense). Primers for unmethylated DNA were: 5¢-GAAGTAGGTGGAGT ATTGAAT-3¢ (sense) and 5¢-CAAAAAAACACAATCCT ACTT-3¢ (antisense). Caspase 3 activity Cells ... (Occ WT ) and variant occludin in apoptosis and invasion, as determined by assay, we revealed that exon 9 played a major role in the induc- tion of mitoc...

Ngày tải lên: 18/02/2014, 18:20

... showed the same mobility as the native enzyme on native-PAGE, and the N-terminal amino-acid sequences of the a1 and b1 subunits of the recombinant enzyme were confirmed to be identical to those of the ... their molecular masses, the molecular a1 : b1 ratio was calculated to be about 1 : 1, which means the enzyme has a heterooctameric (a 4 b 4 ) st...

Ngày tải lên: 20/02/2014, 01:20

... structural information is consid- ered as a reasonable and ideal parameter for ad- dition to the current model. The implementation and the evaluation of the model will be exactly the same as a statistical ... to that of its control sentence. For example, slower reading time was observed in (1 3a) and (1 4a) compared to (13 b) and (14 b) at the disambi...

Ngày tải lên: 20/02/2014, 11:21

... of signals between the states of high and low. (A signal is formula AXc AGb AF(AX (a /x b) ) sense for all paths, at the next state c is true for all paths, globally b is true for all ... and then after one cycle eventually r falls and then after one cycle eventually a falls. which can be rendered in CTL as (4). (4) AG('-,r AAXr ~ AF(- ,a AAX (a...

Ngày tải lên: 20/02/2014, 19:20

... 5¢-ATGGTCTCAAAGGGTGAAGAAGA TAAC-3¢ and P570 5¢-GGATTCTAGACTAGTTTTCCTT CCTCC-3¢. The cpr-6 and mCherry PCR products were fused using P 612 5¢-TGGACCGTTCTCAGAAAGTAA CTCCGC-3¢ and P598 5¢-TTTATGTTTTCTTTTAAAC CTTCCTCC-3¢. ... catalyzes the for- mation of phosphatidylinositol 3,5-bisphosphate and is orthologous to PIKfyve in mammals and Fab1p in yeast [ 21] . Cells lacking t...

Ngày tải lên: 06/03/2014, 09:22

... α-Myc whole FLAG-GST IP: α-FLAG B 1A 2A 3A 4A 5Awt 1A 2A 3A 4A 5Awt whole IP: α-FLAG N10 M10 C10wt C α-FLAG WB: α-Myc FLAG-GST wt N10 M10 C10 A 18 1 200 wt 1A 2A 3A 4A 5A N10 M10 C10 LVCEETANYLVIWCIDLEVE AAAAETANYLVIWCIDLEVE LVCEAAAAYLVIWCIDLEVE LVCEETANAAAAWCIDLEVE LVCEETANYLVIAAAALEVE LVCEETANYLVIWCIDAAAA LVCEETANYL TANYLVIWCI VIWCIDLEVE Fig. ... (Fig. 5A) . Alanine- scann...

Ngày tải lên: 07/03/2014, 21:20

... 82 413 18 E-mail: cczheng@sdau.edu.cn or cawu@sdau.edu.cn (Received 26 January 2 011 , revised 22 April 2 011 , accepted 28 April 2 011 ) doi :10 .11 11/ j .17 42-4658.2 011 .0 814 7.x The molecular mechanism for ... which was assigned a value of 1. Data represent the average of three independent experiments ± standard deviation. Standard errors are shown as bars above the colu...

Ngày tải lên: 14/02/2014, 18:20

... franco.novelli@unito.it (Received 5 November 2 010 , revised 19 January 2 011 , accepted 21 January 2 011 ) doi :10 .11 11/ j .17 42-4658.2 011 .08025.x a- enolase (ENOA) is a metabolic enzyme involved in the synthesis of ... suggests that IgGs against TAA are not only a diagnostic tool, but also allow the selection of TAAs for cancer immunotherapy. In many cancer patien...

Ngày tải lên: 14/02/2014, 19:20