Báo cáo khoa học: The use of synthetic linear tetrapyrroles to probe the verdin sites of human biliverdin-IXa reductase and human biliverdin-IXb reductase potx

... The use of synthetic linear tetrapyrroles to probe the verdin sites of human biliverdin-IXa reductase and human biliverdin-IXb reductase Edward M. Franklin1, ... elevating the concentration of cytosolic biliverdin-IXa. The pau-city of information on the biliverdin binding site hashampered the development of inhibitors of BVR-A.With this in mind, and in the ... proceduresPreparation of biliverdin-IXd dimethyl ester and biliverdin-IXb dimethyl ester The dimethyl esters of biliverdin-IXb, biliverdin-IXc and biliverdin-IXd were synthesized by coupled oxidation of haem,...

Báo cáo khoa học: Combined use of selective inhibitors and ﬂuorogenic substrates to study the speciﬁcity of somatic wild-type angiotensin-converting enzyme docx

... 1 and 2) validates the use of selective inhibitors as convenient tools to study the spe-ciﬁcity of the somatic form of ACE. Thus, with thisapproach, the catalytic property of each domaintowards ... with the N-domain of the mouse and human enzymes, may in part be due to this mutation. The resolution of the 3D structure of the germinal form of ACE has provided a strong impetusfor further ... lower than that for the human N-domain, and Mca-Ala selectivity differs between rat and human ACE. In the human enzyme, the binding of RXP407 to the N-domain led to 80% inhibition of its activity(arrow...

Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... solubleforms of the receptor [25,26,34], indicates that the ectodomain of the receptor is capable of dimer forma-tion in the absence of ligand.Afﬁnity cross-linking of [125I]IGF-II to a mutantrepeat ... a localeffect because binding of PMP-BSA, which binds to other sites in the ectodomain of these heterodimers,resulted in the predicted amount of ligand binding.This loss of binding function ... betweenligand and receptor within the binding pocket. Thisleads to the question as to whether the RﬁA mutationactually causes a loss of binding energy sufﬁcient to abrogate Man-6-P binding or whether...

Tài liệu Báo cáo khoa học: Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics pdf

... cleavages in the center of the protein to formadditional loose ends to be denatured out of the ball of spaghetti. The ESI spectrum of the 200 kDa human comple-ment C4 glycoprotein (of 1714 residues ... beenfound to be speciﬁc and potent HAD inhibitors. To check for covalent modiﬁcations of the enzyme, the effect of the inhibitor on the molecular mass value of HAD was measured; instead of an adduct ... substitution;MS ⁄ MS will then show the substituent positions of dif-ferent isomers. A problem for MS ⁄ MS of either the peptides for the bottom-up approach or of the proteinsfor the top-down approach...

Tài liệu Báo cáo khoa học: Altered deoxyribonucleotide pools in T-lymphoblastoid cells expressing the multisubstrate nucleoside kinase of Drosophila melanogaster doc

... into human cells may affect the metabolism of the target cells inmore ways than just the therapeutic purpose of the introduced gene. The normal function of nucleosidekinases is to provide the ... previous reports. One of the most important differences may be the cell typeused in the different studies. The toxicity of nucleosides,as well as the sensitivity towards cytotoxic nucleosideanalogs, ... engineered XhoI and BamHI restriction enzyme sites were used to clone the open reading frame of Dm-dNKcDNA into the XhoI–BamHI site of the pLEGFP-N1 vector(Clontech, Mountain View, CA, USA). The plasmids...

Tài liệu Báo cáo khoa học: "An Information-Theory-Based Feature Type Analysis for the Modelling of Statistical Parsing" docx

... brother of the parent 0.2505 1.5603 0.61455.2 The analysis to the influence of the structural relation and the structuraldistance to the predictive informationquantities of the history feature ... ConclusionEither of the predictive information quantity of the first word and the second word in the objective word sequence is larger than that of the headword of the parent node which has the largest ... types is, the headword of the currentnode (type1), the headword of the parent node(type2), the headword of the grandpa node(type3), the first word in the objective wordsequence(type4), the first...

Tài liệu Báo cáo khoa học: Casein kinase 2 speciﬁcally binds to and phosphorylates the carboxy termini of ENaC subunits ppt

... between the C tails of b and c and the ubiquitin ligase Nedd4. The WW domains of Nedd4 bind to the proline-rich PY motifs of b and cENaC, leading to channel ubiquitination, internalization and degradation ... quantiﬁed byphosphorimaging of the band corresponding to the full-length protein, and normalizing to the Coomassie bluestaining of the same band.)It was next demonstrated that the kinase eluted infraction ... (Fig. 8A), the inhibitory effects of heparin and DPG (Fig. 8B), the fact that puriﬁed CK2 canphosphorylate bS631 and cT599 (Fig. 7A), the presence of the a subunit of CK2 in the active cytosolic...

Báo cáo khoa học: Endogenous tetrahydroisoquinolines associated with Parkinson’s disease mimic the feedback inhibition of tyrosine hydroxylase by catecholamines doc

... positively charged nitrogen atoms of NMTIQ and MPTP and the backbone oxygen of Ser324. The dis-tances between the nitrogen atoms and the oxygen of Ser324 were 2.01 A˚for NMTIQ and 2.26 A˚forMPTP, ... to produce a major effecton neurotransmitter synthesis. In comparison, up to 104-fold higher concentrations of TIQs are required to cause cytotoxic blockade of the mitochondrial respira-tory ... at the center of the catalytic site enabled bid-entate ligation between the two hydroxyl residues of their catechol moiety and the iron. The unbound ends of these molecules projected from the...

Báo cáo khoa học: 15-Deoxy D12,14-prostaglandin J2 suppresses transcription by promoter 3 of the human thromboxane A2 receptor gene through peroxisome proliferator-activated receptor c in human erythroleukemia cells ppt

... further evidence for the role of PPARc in the regulation of TP gene expression within the vasculature and point to further critical differencesinto the modes of regulation of TPa and TPb inhumans. ... 15d-PGJ2-suppression of Prm3 activity. On the other hand, either deletion of the PPARc (b) and RXR IV half sites, such as in the )154 subfragment, or site-directed mutagenesis of the RXR IV within the )320 ... owing to the inability to transfectHEL cells to high efﬁciency for RNAi and due to the failure of CHIP analysis to discriminate between the PPARc ⁄ RXR site located between )159–148 and the adjacent...

Báo cáo khoa học: N-Methylation in polylegionaminic acid is associated with the phase-variable epitope of Legionella pneumophila serogroup 1 lipopolysaccharide pptx

... group in the other legionaminic acid residues of the OPS. The proton signals of the major higher-®eld pair at dH3.03 and 2.95 correlated in the 1H,13CHMBCspectrumtodifferent nonprotonated ... between the LPS of wild-type R C1 and phase variant 811 [ 24] or between the L PS of the avirulentwild-type 5097 and the corresponding mutant 137 [17]. As the mAb 2625 LPS epitope was found to be ... residues of legionaminic acid was the smallest one that displayed t hese signals and, moreover, the relative intensity of these signals was the highestcompared to other pools from the same strain. Therefore,further...

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