Báo cáo khoa học: High levels of structural disorder in scaffold proteins as exemplified by a novel neuronal protein, CASK-interactive protein1 pot

... anticipated that structural disorder may be a general feature of scaffold proteins. In a recent review, structural disorder in several scaffold proteins and in other proteins of multiple binding partners ... introductory paragraphs, the N-terminal half of Caskin1 contains a number of well-known domains involved in protein–protein inter- action, such as...

Ngày tải lên: 16/03/2014, 02:20

... histidine and carboxylate (aspartate in chymotrypsin, glutamate in cholinesterases) of the cata- lytic triad. Compression of the His-Asp/Glu diad causes the basicity of the histidine to increase, ... interested in the molecular basis of substrate activation in wild-type and mutants of human BuChE [11,15,16], as well as substrate activation in wild-type and mutants of...

Ngày tải lên: 20/02/2014, 23:20

... 2737 Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the ... categorized into five groups: (a) perinatal hypophosphatasia, (b) infantile hypophos- phatasia, (c) childhood hypophosphatasia, (d) adult hypophosphatasia and (e) odonto hypophosphata...

Ngày tải lên: 07/03/2014, 05:20

... presence of an aspartate or asparagine residue adjacent to the acid ⁄ base catalyst of the xylanase, and therefore to the pH optimum of the xylanase. The lower the pH optimum of the xylanase, the ... the main-chain carbonyl oxygen of Phe375 TAXI-IA . A further cascade of conformational changes upon association of TAXI-IA and BSX is observed in the aglycon-binding sites of...

Ngày tải lên: 23/03/2014, 05:22

... precipitation of a- lactalbumin commenced after 25 min and reached a plateau after 90 min. The amount of DTT-induced aggregation of a- lactalbumin was increased in a concentration-dependent manner by the ... was found to specifically increase the activity of aB-crystallin against DTT-induced precipi- tation of insulin at intermediate and high concentra- tions, and it also...

Ngày tải lên: 18/02/2014, 16:20

... RNase AA-G; (m) RNase AA; (·) RNase A. Fig. 1. Aggregation of DMPG vesicles induced by RNases. Sym- bols: (e) BS-RNase; (+) RNase AA-GG; (n) HHP-RNase; (n) RNase AA-G; (h) RNase AA; (m) HP-RNase; ... cationic proteins with cytotoxic activ- ity: BS-RNase [10] is a natural dimeric RNase; RNase- AA [21], RNase-AA-G [21], and RNase-AA-GG [22] are dimeric variants engineered from bovine...

Ngày tải lên: 19/02/2014, 06:20

... The main fraction contained a 75 kDa matriptase as a major component and a few contamin- ating proteins as analyzed by SDS ⁄ PAGE. RNAi experiments with OVISE cells Matriptase siRNAs and a scrambled ... of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substrates. J Biol Chem 275, 26333–26342. 34 Ihara S, Miyoshi E, Nakahara S, Sakiy...

Ngày tải lên: 19/02/2014, 07:20

... transfectants was used for GABA-uptake assay, and another aliquot was used to determine the quantity of plasma membrane pro- teins of GAT1 and mutants (see above). The relative amounts of plasma ... of plasma membrane proteins of GAT1 or mutants were analyzed by imager scanning of western blots. The GABA-uptake activity was normalized with the same amount of plasma membrane...

Ngày tải lên: 19/02/2014, 17:20

... 313 create one classifier for each type of label using instances with that label as positive instances and instances with any other label as negative instanc- es. The final class label is assigned ... not asso- ciated with the patient. 3 The Assertion Classifier We approach the assertion classification task as a supervised learning problem. The classifier is giv- en a medica...

Ngày tải lên: 20/02/2014, 05:20

... Proceedings of the 47th Annual Meeting of the ACL and the 4th IJCNLP of the AFNLP, pages 324–332, Suntec, Singapore, 2-7 August 2009. c 2009 ACL and AFNLP 1 2 3 1 1 2 3 { } 324 X ...  X a X d X b X a X b X d ≺ X a ≺ X b ≺ X c ≺ X d ≺ X e X d ≺ X a ≺ X b ≺ X c ≺ X e X d     ≺  ≺   ≺  ≺    d(Y  , Y  ) 326      ❄ ❳ ❳ ❳ ❳ ❳③ ✘ ✘ ✘ ✘ ✘✾ ❄❄ ❄ ❄ X a ⇒ .....

Ngày tải lên: 20/02/2014, 07:20