Báo cáo khoa học: Modeling of ATP–ADP steady-state exchange rate mediated by the adenine nucleotide translocase in isolated mitochondria potx

... 2009 The Authors Journal compilation ª 2009 FEBS 6955 Modeling of ATP–ADP steady-state exchange rate mediated by the adenine nucleotide translocase in isolated mitochondria Eugeniy Metelkin 1 , ... Effect of nigericin on ATP–ADP steady-state exchange rate mediated by ANT. Bar graph of ATP–ADP steady-state exchange rate mediated by AN...

Ngày tải lên: 16/03/2014, 00:20

... M (2004) Kinetics and binding sites for interaction of the prefol- din with a group II chaperonin: contiguous non-native substrate and chaperonin binding sites in the archaeal prefoldin. J Biol ... aggregation in vitro. PFD is thought to play a general role in de novo protein folding in archaea, and in the biogenesis of actin, tubulin and possibly other proteins in the...

Ngày tải lên: 07/03/2014, 04:20

... moiety of A76 maintains the appropriate orientation of the carboxyl group of the amino acid for Pa. On GluRS, in the absence of tRNA, the K m value of Glu in the ATP–PP i exchange reaction increases ... A ˚ . These distances within 0.9 A ˚ of the distance of 12.8 A ˚ in ArgRS indicate that, in these aaRSs, this space is the binding site of the adeno...

Ngày tải lên: 18/02/2014, 11:20

... ADP-ribosylation of EF-2 in the mitochondrial apoptotic response induced by the toxin remains to be determined. Finally, we assign an important role to the endosomal acidic cathep- sins B and D in increasing ... protease cathepsin D, as indicated by the following observa- tions: (a) the inhibitor profile of the endosomal ETA-degrading activity was very similar to that...

Ngày tải lên: 18/02/2014, 04:20

... 6. The ATP-binding site in the MD simulated model structure of the binary hGK–ATP complex and the domain motion induced by ATP binding to the hGK apoenzyme. (A) Close-up view of the ATP- binding ... change induced by the binding of adenine nucleotides to human pancreatic GK, as determined by intrinsic tryptophan fluorescence, using the catalytically inactive mut...

Ngày tải lên: 06/03/2014, 00:20

... stimulated during growth by changing the size of the polysomes [14]. This could occur either by moving the 40S ribosomal subunit at a faster rate along the 5¢-UTR, or by increasing the rate of elongation. ... Fine tuning of these steps is achieved by regulating the interaction of these initiation factors with each other and the mRNA [1]. PABP1 is an RNA-bindi...

Ngày tải lên: 18/02/2014, 12:20

... of alternative promoters in DNASE1 results in no generation of protein isoforms. The 5¢-UTR of eukaryotic mRNA in uences the initi- ation step of protein synthesis and thereby in part determines ... expression in vivo or in vitro, including characterization of the promoter region of the gene and the associated transcriptional factors. Therefore, delineation o...

Ngày tải lên: 19/02/2014, 06:20

... anisomycin or FBS and further incubating the cells with actinomycin D (10 lgÆmL )1 ) for the indicated periods. Likewise, the role of RhoA and p38 signaling was examined by preincubating the ce ... prompt termination of the pr otein s ynthesis. These data a dd to the growing body of information supporting a preponderant role of p38 in the regulation of gene express...

Ngày tải lên: 19/02/2014, 16:20

... in the N-terminal Mad homology 1 domain of all Smads binds to importin-b in the case of Smad3 and importin-a in the case of Smad4, while mutation of the NLS blocks the ability of these proteins ... implies the involvement of non-Smad pathways, in certain cases it was found that the Smad pathway may also play a role in the early activation of Rho protein...

Ngày tải lên: 07/03/2014, 01:20

... determi- nants required for binding of each of these proteins. Four point mutations targeting the residues C-terminal of Tyr1138 were generated to test their potential role in binding of the different STAT ... signalling. Of the two other intracellular tyrosine residues in LEPRb, Tyr985 can recruit either the tyrosine phosphatase SHP-2 or suppressor of cyto- kine signa...

Ngày tải lên: 07/03/2014, 16:20