Báo cáo khoa học: Site-directed enzymatic PEGylation of the human granulocyte colony-stimulating factor pptx

... number of poten- tial substrates during the screening of the full human proteome. This highlights the importance of factors other than the extended cleavage specificity in deter- mining whether ... cells that are dis- tributed along the surfaces of the body. They are fre- quently found in the mucosa of the airways and intestine, in connective tissue of the skin,...

Ngày tải lên: 22/03/2014, 21:21

... that the mutations primarily affect binding of the substrates, but they do not rule out the possibility of a reduced access of the substrate to the catalytic site upon mutation. On the other ... apparent affinity of the mutant. For both substrates, the mutation decreased the V max values. On the other hand, the V max of the mutant towards 17-epiestriol was sli...

Ngày tải lên: 23/03/2014, 09:21

... that one of the others becomes the PEGylation site. To verify which of the glutamines of Mut4 are transglutaminated, the PEGylation sites of native and mutated G-CSF were analyzed by enzymatic ... with the calculations performed on the mutant. We are led to conclude that PEGylation of one of the two glutamines impairs the PEGylation of the neighboring...

Ngày tải lên: 16/03/2014, 00:20

... presence of alternative splicing around the 5¢-end of exon 6 of Meis2 and Meis3 was tested by RT-PCR. The positions of molecular mass markers are shown to the left, and the size in base pairs of the ... However, the activity of the full-length Meis1a was not tested, and on the basis of our work we expect that its activity would be inhibited by the conserved Hth...

Ngày tải lên: 16/02/2014, 15:20

... increases the transcription but not the secretion of IL-1b and TNF-a, suggesting that the roles of these factors in the paracrine effects of MSCs are negligible. However, hypoxia ⁄ SD also enhances the ... and secretion of IL-10 Because of the lack of secretion of the inflammatory cytokines IL-1b and TNF-a from hypoxia ⁄ SD-stimu- lated MSCs, as well as the signifi...

Ngày tải lên: 18/02/2014, 04:20

... advantage of p53 mutation analysis, and a unique feature of this database, is the availabil- ity of the residual activity of the majority of p53 mis- sense mutants. The biological activity of mutant ... of a severe mutant. At the other end of the spectrum, where we have low energy, there is 75% probability for the mutation to be nonsevere if the energy is 0.125...

Ngày tải lên: 18/02/2014, 11:20

... obtained with the transition at posi- tions 1915 and 1917. These mutations had an effect on the formation of pseudouridine exclusively at the mutant position and did not affect either of the other two ... by Southern analysis. The rluD::cat strain had a full deletion of both rluD and yfiH, together with their annotated pro- moter sequences. The inserted cat cassette had a dis...

Ngày tải lên: 18/02/2014, 16:20

... Given that the roles of these residues definitely confirm each other, the dif- ference in their significance might be attributable to the importance and ⁄ or necessity of the receipt of the phenol-hydroxyl ... environments of BPA in the ligand-binding pocket of the ERRc. The proximity of each amino acid residue (within a distance of 5 A ˚ ) to BPA is shown in the...

Ngày tải lên: 18/02/2014, 16:20

... each extremity of the polypeptide chain, must of necessity arise from contacts that are made outwith the active site of the enzyme. The effect of removal of the N-terminal extension The full N-terminal ... binding of this helical N-terminal seg- ment of IA 3 . The E + I « EI balance thus lies far to the right and the addition of further residues at the N-te...

Ngày tải lên: 19/02/2014, 00:20

... less sensitive to the pres- ence of the anion. These residues also modulate the conformation of unfol- ded cytochrome c, influencing its spin state and the coordination to the prosthetic group. Abbreviation CT, ... effect of Cl – reflects the different affinities of the two anions for the protein [11,17]. We also monitored the effect of sulfate on the perchlorate-in...

Ngày tải lên: 19/02/2014, 05:20