Báo cáo khoa học: Membrane type-1 matrix metalloprotease-independent activation of pro-matrix metalloprotease-2 by proprotein convertases docx

... decrease of the mature enzyme form is specific for TACE, as the amount of mature ADAM10 was unaffected in PMA-treated HEK293 and SH-SY5Y cells. Our results indicate that the activation of TACE by the proprotein- convertases ... processing of TACE by overexpression of these convertases in HEK293 cells. We were able to show, that both proprotein- conver- tases contribute to...

Ngày tải lên: 08/03/2014, 02:20

... characterization of HpSDH demonstrates its activity with k cat of 7.7 s )1 and K m of 0.148 mm toward shikimate, k cat of 7.1 s )1 and K m of 0.182 mm toward NADP, k cat of 5.2 s )1 and K m of 2.9 mm ... Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China 2 School of Pharmacy, East China Univer...

Ngày tải lên: 19/02/2014, 05:20

... be synthesized is 35% of the total amount of plasma membrane proteins, in comparison with the level of H + -ATPase which represents 20–50% of the total amount of plasma membrane proteins [23,24], and ... Pdr12 became one of the most abundant plasma membrane proteins [22], whereby the measured level of Pdr12 was comparable to the level of H + - ATPase. Assuming that the l...

Ngày tải lên: 07/03/2014, 04:20

... followed by an increase in ferritin synthesis [37]. Such NO-dependent IRP2 degradation was caused by the S-nitrosylation of Cys178 which led to ubiquitination of the protein fol- lowed by its degradation ... Fer- ritin was immunoprecipitated by using anti-ferritin IgG and analyzed by SDS ⁄ PAGE followed by autoradiography. Fig. 6. Polysome profiles of mRNAs isolated by sucr...

Ngày tải lên: 07/03/2014, 12:20

... or grammaticalization, is a process of linguistic change by which a content word becomes part of the grammar by losing its lexical and phonological load. 7 By full NPs we mean NPs with a nominal ... resolution of anaphoric full NPs, i.e. those semantically dependent on a previous mention. 2 We follow the ACE terminology (NIST, 2003) but in- stead of talking of objects in the w...

Ngày tải lên: 08/03/2014, 21:20

... stimulation of PC12 cells results in activation of the Smad pathway independently of TGF-b1. This activation is direct and results in nuclear translocation of Smad3 within only 30 min of NGF treatment. ... phosphory- lation of the C-terminal SSXS-motif of the R-Smad. Based on the diverse mechanism of Smad activation by either TGF-b1 or NGF, specific subsets of target...

Ngày tải lên: 16/03/2014, 16:20

... system's performance in terms of cov- erage and quality of translations by combining frag- mentary analyses when no spanning analysis of the input can be derived because of spontaneous speech phenomena ... greater length is preferred. The coverage of an analysis is 1405 the sum of the lengths of the component analyses it consists of. Note that the coverage of a...

Ngày tải lên: 17/03/2014, 07:20

... >2 induced by low concentrations of denaturants, we investigated the effect of NaCl on the formation of O n >2 by denatur- ing PARN in buffers with the addition of various amounts of NaCl. A ... Yan State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China The control of...

Ngày tải lên: 23/03/2014, 04:21

... formation of a distinct peptide of 27 kDa after trypsin treatment of cells indicates the existence of one large extension protruding at the external side of the plasma membrane. From the size of the ... susceptibility of the isolated membrane to the enzyme suggest that C-terminal of LBP may be oriented intracellularly. Intramembranous domain of LBP To identify the intrame...

Ngày tải lên: 21/02/2014, 00:20

... occurs via cleavage of the propeptide by membrane type-1 matrix metalloprotease. However, significant residual activation of pro -matrix metalloprotease-2 is seen in membrane type-1 matrix meta- lloprotease ... type-1 matrix metalloprotease-independent activation of pro -matrix metalloprotease-2 by proprotein convertases Bon-Hun Koo, Hee-Hyun Kim, Mich...

Ngày tải lên: 16/03/2014, 00:20