Báo cáo khoa học: The tetralogy of Fallot-associated G274D mutation impairs folding of the second epidermal growth factor repeat in Jagged-1 potx

... vi- tro induced by the G274D mutation, the misfolding of the G274S and G274A mutants, the impossibility of rescuing the G274D mutation with compensatory mutations, the sensitivity of the G274D ... folded. The results obtained in the present study suggest the opposite. There are several lines of evidence in support of this hypothesis: the dramatic i...

Ngày tải lên: 16/03/2014, 00:20

... conformation, the SH2 domain binds to pY i on the C-terminal tail, and the Src homology 3 (SH3) domain binds to the linker between the SH2 and kinase domains at the back of the small lobe, preventing the ... by presenting the pulse of Src activity in the plane of the inactive and active fractions, s i and s 1 . If the duration of the stimulus exceeds the...

Ngày tải lên: 18/02/2014, 11:20

... composition, indicating that the peptide is of the correct sequence and free of contaminating pro- teins. Five cycles of N-terminal sequencing confirmed the expected residues including the presence of methio- nine ... : 2000 in blocking solution for 30 min. Cells were rinsed in NaCl ⁄ P i several times and incubated in blocking serum containing anti-mouse IgG (Vectastain)...

Ngày tải lên: 18/02/2014, 13:20

... transcriptional initi- ation sites. Determining the translation initiation site of isoform 2 KLK11 Messenger RNA for isoform 2 contains two initiation codons near the 5¢ end. To examine whether the first initiation ... signal peptide of isoform 1 (Fig. 2A) [8]. The second initi- ation codon is thought to be an actual translation initiation site of isoform 2, instead of the...

Ngày tải lên: 19/02/2014, 06:20

... important for the second- half reaction and are part of the active-site of CoaB; these residues may be directly involved in binding of one of the substrates, the amino acid cysteine (Fig. 3). ... cysteine. The synthesis of PPC occurs in two half reactions starting with the formation of 4¢-phosphopantothenoyl-CMP (activation of the carboxyl group of 4¢-phosph...

Ngày tải lên: 19/02/2014, 12:20

... cycles in such a combination do not touch each other). The value (the sign) of a cycle is the product of the values (the signs) of its steps. The sign o f the magnitude of a Ôminus combined autoinfluence ... concentrations of its species. Therewith the c oefficient of o rder p of the characteristic equation equals the sum of all simplest combined Ôminus autoi...

Ngày tải lên: 19/02/2014, 16:20

... (F340) increases, whereas the fluorescence intensity at 380 nm (F380) decreases upon the binding of Ca 2+ . The change in the ratio of F340 ⁄ F380 determined the change in Ca 2+ concentration inside ... Ca 2+ ⁄ CaM-binding IQ domain that is involved in the activa- tion of Ras-GRF1. Stimulation of all the splice variants of the G s -coupled serotonin receptor 5-h...

Ngày tải lên: 19/02/2014, 18:20

... sequences of HS chains are de®ned for the bFGF binding and for the recognition of the speci®c bFGF receptor, leading to the formation of a ternary complex comprising HSPG±bFGF±FGFR. These oligosaccharidic motifs ... FGF at the transcriptional l evel. The bFGF inhibitory eect was also observed in the presence of dibu- tyryl-cAMP, ch olera toxin or R O-20-1724, all indu...

Ngày tải lên: 21/02/2014, 03:20

... aldosterone and insulin, as well as intracellular signalling components such as protein kinase C and protein kinase A, were found to increase the in vivo phosphorylation of the C termini of both b ... by phosphorimaging of the band corresponding to the full- length protein, and normalizing to the Coomassie blue staining of the same band.) It was next demonstrated that t...

Ngày tải lên: 21/02/2014, 03:20

... Microinjection of NLS-containing substrates in a myoblast. The movie represents the NLS microin- jection experiment shown in Fig. 6A. Video S2. Microinjection of NLS-containing substrates in a ... cultured in 1.2 mgÆmL )1 puromycin-containing DMEM supplemented with 10% fetal bovine serum. After confirming their ability to differentiate, cells were maintained in 1.0 mgÆmL )1 p...

Ngày tải lên: 06/03/2014, 01:20