Báo cáo khoa học: An engineered right-handed coiled coil domain imparts extreme thermostability to the KcsA channel docx

... FEBS An engineered right-handed coiled coil domain imparts extreme thermostability to the KcsA channel Zhiguang Yuchi 1 , Victor P. T. Pau 2 , Bridget X. Lu 1 , Murray Junop 1 and Daniel S. C. Yang 1 1 ... a left-handed coiled coil, but the bundle crossing on KcsA is a right-handed coiled coil (RHCC). We hypothesized that the splicing of two different...

Ngày tải lên: 16/03/2014, 00:20

... h and R as Boltzmann’s constant, Planck’s constant and gas constant, respectively, and T and DG # as absolute temperature and Gibbs free energy of activation, respectively, the DG # of unfolding ... above, that the intro- duction of the disulfide bridge stabilizes the molecule more than calcium ions. The influence of isopropanol on the unfolding rate constant To measure unfolding...

Ngày tải lên: 19/02/2014, 17:20

... send only an ID of a TFS. Only when the ID is passed to other CSAs and they try to modify a TFS with the ID, the actual transfer of the TFS's real image occurs. Since the transfer is ... the name-concatenator waits for the name-concatenator-sub to return concatenated names, and puts the return val- ues into the variable R. The CA name-concatenator contro...

Ngày tải lên: 20/02/2014, 18:20

... but retains the coiled- coil region, was reported by Wang et al. [84]. This isomer cannot translocate to the nucleus and has been proposed to be a negative regulator of Par-4 apoptotic activity ... region N-terminal to the leucine zipper domain provides an electrostatic surface to counter the negative charges in the leucine zipper domain or otherwise helps to stab...

Ngày tải lên: 16/03/2014, 02:20

... acceptors The kinetic constants for the two artificial electron acceptors cytochrome c and NBT, using NADH as the electron donor, were determined from Hanes–Haldane plot in the presence of either ... The numbers on the left represent the molecular masses (kDa). Lane A: low molecular mass standards; lane B: crude extract; lane C: after anion exchange chromatography on a DE 52-cell...

Ngày tải lên: 21/02/2014, 00:20

... connected by an intrarepeat loop, and m utations in distinct lo ops alter the binding of the B and C subunits [13]. The B subunits bind to repeats 1±10 of the A subunit, whereas the C s ubunit binds to ... about the molecular basis for the interaction of the B subunits with the AC heterodimer. None of the B subunits have been mapped to de®ne the Correspondence t...

Ngày tải lên: 08/03/2014, 10:20

... and 40%), the Department of Anesthesia Kantosspital Basel, HPRN-CT-2002-00331 from the European Union, the Italian Space Agency and the Swiss Muscle Foundation. R. Gambari is supported by grants ... transcription factor binding to the P1 promoter sequences of the human AbH-J-J locus Giordana Feriotto 1 , Alessia Finotti 2 , Giulia Breveglieri 1 , Susan Treves 3 , Francesco Zorza...

Ngày tải lên: 23/03/2014, 07:20

... gene into these target tissues. On the other hand, there is the COX-1 enzyme, which is well known to have a similar function (coupling to PGIS to synthesize PGI 2 in vitro and in vivo) to that ... compared to that of the previously engineered Trip-cat enzyme-2. Furthermore, an HEK293 cell line that can stably express the active new Trip-cat enzyme-1 and constantly synthe...

Ngày tải lên: 30/03/2014, 02:20

... (ortho- logue of human RPE65) and retains all of the known key residues for the enzymatic activity of RPE65. Similar to the other RPE-specific RPE65, RPE65c was present in both the membrane and cytosolic ... [11,19–21]. Cone photoreceptors have faster responses to light than rod photoreceptors and thus demand more chro- mophore supplies [22,23]. It has been suggested that the con...

Ngày tải lên: 14/02/2014, 14:20

... the toxin, and cleaves six members of the mitogen-activated protein kinase kinase family after being delivered to the cytosol by the cell- binding component of the toxin, protective antigen. The ... 9500 g for 30 min, and the supernatant was decanted. The pellet was resuspended in 10 mL of supernatant, and then centrifuged at 20 000 g for 30 min. The supernatant was decant...

Ngày tải lên: 16/02/2014, 09:20