Báo cáo khoa học: Improvement of ecdysone receptor gene switch for applications in plants: Locusta migratoria retinoid X receptor (LmRXR) mutagenesis and optimization of translation start site pptx

... FEBS Improvement of ecdysone receptor gene switch for applications in plants: Locusta migratoria retinoid X receptor (LmRXR) mutagenesis and optimization of translation start site Ajay K. Singh 1,2 , ... DBD, DNA-binding domain; EcR, ecdysone receptor; FMV, figwort mosaic virus; HsRXR, Homo sapiens retinoid X receptor; LBD, ligand-binding...

Ngày tải lên: 15/03/2014, 23:20

... domain (AD) was fused to the LBD of Locusta migratoria retinoid X receptor (LmRXR) or Homo sapiens retinoid X recep- tor (HsRXR). The ligand sensitivity of the EcR gene switch was improved by using ... ecdysone receptor; FMV, figwort mosaic virus; HsRXR, Homo sapiens retinoid X receptor; LBD, ligand-binding domain; LmRXR, Locusta migratoria retinoid X...

Ngày tải lên: 16/03/2014, 06:20

... the expression of genes such as TNF, MAF, Rab and Reprimo. The AMK ligand induced changes in the expression of genes such as v-jun, FBJ and EGR. 20-Hydroxyecdysone (20E) did not affect gene expression, ... regulated expression of the transgene finely tuned to the chang- ing clinical state of a patient. Several gene switches have been developed for regu- lating the expressio...

Ngày tải lên: 30/03/2014, 03:20

... the per- formance of WSME models trained using MCMC and WSME models trained using PS. Features of n-grams and features of triggers were used In both kinds of models, and the WSME model trained with ... in the derivation of . To include the rules of the form , where and , in the set , we make use of a special symbol $ which is not in the terminals nor in the non...

Ngày tải lên: 23/03/2014, 19:20

... (solid line) and low-pH buffer-exposed (dashed line) IVIg to Escherichia coli antigens. Migration distances (x- axis) expressed in pixels were plotted against the intensity of binding (y-axis) expressed ... Increased binding of low-pH buffer-exposed IVIg to human factor H. In both panels, binding of the native IVIg is indicated by a solid line, and that of the low-pH buffer-ex...

Ngày tải lên: 16/02/2014, 15:20

... linker region and ligand-binding domain (LBD) of ecdysone receptors. Fig. S2. Amino acid alignment of linker region and ligand-binding domain (LBD) of ultraspiracle ⁄ RXR receptors. This supplementary ... Windler DA, Pawlak-Skrezecz A, Eaton RE et al. (2005) The X- ray structure of a hemipteran ecdysone receptor ligand- binding domain: comparison with a lepidopteran ecdyso...

Ngày tải lên: 18/02/2014, 08:20

... Molecular cloning of the family of glutamine synthetase genes from maize: expression of genes for glutamine synthetase and ferredoxin-depen- dent glutamate synthase in photosynthetic and non- photosynthetic ... discerned the specific role of the isoproteins of ferredoxin and ferredoxin:NADP + oxidoreductase in providing ferredoxin-GOGAT with photoreduced or enzymatically...

Ngày tải lên: 18/02/2014, 18:20

... Olesen H (1971) Nature of vitamin B 12 binding. II. Steric orientation of vitamin B 12 on binding and number of combining sites of human intrin- sic factor and the transcobalamins. Biochim Biophys Acta ... concentration of the binding sites. Binding of IF to the receptor IF, with or without ligands, interacted with the specific receptor cubilin immobilized on the surface o...

Ngày tải lên: 19/02/2014, 05:20

... were increased in response to EGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinase kinase, inhibited the EGF-induced c-Fos and c-Jun expression, AP1 DNA binding, Bcl -X L expression, ... determine the expression of Bcl -X L , Bad, and Bax by immunoblot- ting (Fig. 3A). EGF increased the expression of Bcl- X L , but not that of Bad or Bax. As for Bcl-2,...

Ngày tải lên: 19/02/2014, 06:20

... gazetteers, and text mining projects for extracting causal knowl- edge, properties of named entities, and opinions. Yet these familiar categories make up only about 40% of the world knowledge used in ... classi- fication of the knowledge. Functional relationships R imply that if x and x  have different values y and y  , then x and x  must be distinct, or mutually...

Ngày tải lên: 20/02/2014, 05:20