Báo cáo khoa học: CHIP participates in protein triage decisions by preferentially ubiquitinating Hsp70-bound substrates pdf

... ubiquitin themselves but generally bring substrates and E2 ubiquitin-conjugating enzymes in close proximity by binding to both proteins. It has been shown that CHIP has the ability to bind substrates ... more regulatory role by interacting mainly with Hsp90 to ubiquitinate signaling proteins. It has been shown in vitro and in cell culture that sev- eral Hsp90 clients are ubiqu...

Ngày tải lên: 15/03/2014, 23:20

... dramatically by 24 h. Expression of caspase-3 and caspase-9 remained unchanged in the presence of PMA/ionomycin. Interestingly, in DT40, PMA/ionomycin treatment increased the caspase-8 mRNA level by 3 ... hand, in E2A )/) , PMA/ionomycin treatment did not in uence the protein level of survivin by 8 h and thereafter it decreased gradually. PMA/ion- omycin treatment did not chang...

Ngày tải lên: 16/03/2014, 04:20

... histone-binding proteins remains still unknown [15,16]. As mentioned above, the histone N-terminal tails are involved in protein binding. They are accessible both in the nucleosome [2] and in chromatin [17] ... diluted in immu- nostaining buffer containing 0.05% Tween 20 and 5% fetal bovine serum was used. After rinsing with immunostaining buffer, sections were further rinsed in d...

Ngày tải lên: 08/03/2014, 10:20

... The protein levels of Ca 2+ -regulated protein kinase Ca were decreased in AJRP parkin mutant cells. Neomycin and dantrolene both decreased the intracellular Ca 2+ levels in parkin mutants in ... functions of the protein remains unknown at present. In support of the first idea, parkin has been shown to protect against the neuro- toxicity induced by unfolded protein stress, su...

Ngày tải lên: 16/03/2014, 03:20

... PDZ proteins: Mint-3, AMPA binding protein and gluta- mate receptor interacting protein (GRIP). It would therefore be interesting to determine whether this motif is also involved in the interaction ... 1 matrix metalloproteinase cytoplasmic tail-binding protein- 1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regula...

Ngày tải lên: 18/02/2014, 04:20

... (2008) Mapping alpha-helical induced folding within the intrinsically dis- ordered C-terminal domain of the measles virus nucleo- protein by site-directed spin-labeling EPR spectroscopy. Proteins 73, ... disordered C-terminal domain of the measles virus nucleoprotein interacts with the C-terminal domain of the phosphoprotein via two distinct sites and remains predominantly unfolded. Prot...

Ngày tải lên: 18/02/2014, 04:20

... engineer increased protein protein interaction stability at will; in particular, the ability to increase stability by kinetic design. For example, by achieving this predominantly by decelerating ... synthesized for analysis, extending the number of interactions from 10 to 45, permitting a rigid interpretation in distinguish- ing interacting from noninteracting proteins. One Fig. 1...

Ngày tải lên: 18/02/2014, 13:20

... observation that heparin does not inhibit FVa inactivation by recombinant APC variants that are unable to bind heparin supports the mechanism in which heparin binding to the basic cluster in APC is responsible ... Heparin and APC-catalyzed inactivation of FVa (Eur. J. Biochem. 271) 2735 30. Fernandez-Recio, J., Totrov, M. & Abagyan, R. (2002) Soft protein protein docking in inte...

Ngày tải lên: 19/02/2014, 13:20

... (Fig. 4A). Inter- estingly, neither inhibiting phosphorylation of Ser916 by mutating the amino acid to alanine, nor poten- tially mimicking it by mutation to aspartic acid or glutamic acid, in uenced ... kinase (ERK) – cascade. The serine ⁄ threonine kinases ERK1 and ERK2 are activated by dual phosphorylation by the MAP kinase kinase, MEK, which becomes phosphorylated and activated...

Ngày tải lên: 19/02/2014, 18:20

... The protein kinase C pseudosubstrate peptide (PKC19-36) inhibits insulin-stimulated protein kinase activity and insulin-mediated translocation of the glucose transporter glut 4 in streptolysin-O ... homology 2 domains (a) the growth factor receptor binding protein activates the ras/ mitogen-activated protein kinase pathway and (b) phosphatidylinositol 3-kinase activates the protein...

Ngày tải lên: 20/02/2014, 02:21