Báo cáo khoa học: Large conformational changes in the Escherichia coli tryptophan synthase b2 subunit upon pyridoxal 5¢-phosphate binding pot

... result indicates that the hydrophobic interaction at the subunit interface of the dimer increases with PLP binding. Upon binding of PLP to the active site of one b subunit (bA), the side chain of ... K (2005) Conformational changes in the a -subunit coupled to binding of the b 2 -subunit of tryptophan synthase from Escherichia coli: crystal structure...

Ngày tải lên: 15/03/2014, 11:20

... whereas insertion F45 disrupts a8. These insertions are likely to affect the positioning of the arginine finger in site D, thereby blocking DNA binding by RFC–PCNA. Of the remaining three inactivating ... an arginine finger and the finger protrudes into the ATP -binding site of the neighbouring subunit. The exact biochemical roles of the arginine fingers have not been precis...

Ngày tải lên: 07/03/2014, 02:20

... possible in order to minimise oxidative damage. There are two conceivable hypotheses how dmeglob1 may be involved in such scenario. On the one hand, dmeglob1 may be directly involved in the enzymatic decomposition ... pro- tein exhibits ligand -binding properties and expression patterns that resemble those of other known insect globins has actually suggested a common, conserved fun...

Ngày tải lên: 30/03/2014, 02:20

... process involving multiple enzymes and retinoid -binding pro- teins between photoreceptors and retinal pigment epi- thelium (RPE); it is essential for maintaining normal vision [3,4]. The key step in ... RPE65c in the inner retina, including Mu ¨ ller cells, but not in the RPE. Furthermore, double-immuno- staining of dissociated retinal cells using antibodies for RPE65c and gluta...

Ngày tải lên: 14/02/2014, 14:20

... 2007) doi:10.1111/j.1742-4658.2007.06051.x The mechanisms underlying increased cardioprotection in younger female mice are unclear. We hypothesized that serine-threonine protein kinase (protein kinase B; Akt) triggers ... that these changes occur in an Akt-independent manner. Further studies are therefore required to identify the precise signaling mechanisms that control the metab...

Ngày tải lên: 18/02/2014, 16:20

... integrin-dependent cell volume sensing and signaling integrates into the overall context of insulin signaling. Similarly, sensing of glutamine-induced hepatocyte swelling by integrins feeds into ... hepatocyte swelling [12,13], insu- lin-induced hepatocyte swelling is registrated by the integrin system, leading to a Src-dependent activation of the p38-type MAPK and thereby inhibition of...

Ngày tải lên: 18/02/2014, 16:20

... of the c ring, respectively. The C-terminal helices show a clear handedness, and two of the rings face in the opposite direction in the membrane to the other two, forming the same pattern as in ... of the ion -binding site. Therefore, the c ring of A. woodii has only 10 membrane-buried negative charges that are essential for binding the ion and also for the rota...

Ngày tải lên: 18/02/2014, 17:20

... Follistatin, Chordin and Noggin [12]. These proteins are antagonists of BMP and other members of the TGF-b family, binding to and inhibit- ing these signaling molecules from binding to their receptors ... for some time. Other factors affecting the BMP pathway Factors inhibiting BMP2b, other than direct binding proteins such as Noggin and Chordin, also affect dorsoventral patterning....

Ngày tải lên: 19/02/2014, 00:20

... membrane protein with its N-terminal FAD -binding domain facing the intermembrane space. This topology would enable Gut2 to transfer of electrons to Nde1 in the intermembrane space [32]. Alo1 catalyses the ... material (P). Proteins were then analyzed by immunoblotting after SDS ⁄ PAGE using antisera against the matrix-located mtHsp70, the membrane protein porin and GFP. Integral...

Ngày tải lên: 19/02/2014, 07:20

... categories. M ethod C * there is telephone and the books on the desk. Eb: The word in the beginning of the part which should be replaced. Ee: The word in the middle or the end of the part which ... word and the one preceding/ the targeted word and the one following/ the targeted word and the two preceding/ the targeted word and the two fol- lo...

Ngày tải lên: 20/02/2014, 16:20