Báo cáo khoa học: Nigrocin-2 peptides from Chinese Odorrana frogs – integration of UPLC/MS/MS with molecular cloning in amphibian skin peptidome analysis pot

... Odorrana frogs – integration of UPLC/MS/MS with molecular cloning in amphibian skin peptidome analysis Lei Wang 1 , Geisa Evaristo 2 , Mei Zhou 1 , Martijn Pinkse 2,4 , Min Wang 1 , Ying Xu 1 , Xiaofeng ... residues in (A) nigrocin-2- related peptides from O. sch- mackeri skin, (B) nigrocin-2- related peptides from O. grahami skin, and (C,D) identic...

Ngày tải lên: 15/03/2014, 10:20

... array of linear a-helical antimicrobial peptides that are 1 9–3 4 residues long (Table 1). They include the dermaseptins B and dermaseptins S [3 0–3 3], phylloxin [34] and dermatoxin [35] from frogs of ... hypervariability of skin anti- microbial peptides and the proposed mechanisms of diver- sification could be part of a strategy for providing frogs with a maximum...

Ngày tải lên: 23/03/2014, 17:21

... Gaudin C & Be ´ laı ¨ ch JP (1999) Sequence analysis of scaffolding protein CipC and ORFXp, a new cohesin-containing protein in Clostridium cellulolyticum: comparison of various cohesin domains ... [17]. In C. cellulolyticum, the scaffolding protein contains eight cohesin modules that potentially bind to 62 dock- erin-bearing proteins. Previous studies have suggested that any Ci...

Ngày tải lên: 18/02/2014, 08:20

Ngày tải lên: 08/03/2014, 01:20

... agreement with the predicted molecular mass of the hexahistidine fusion protein (27 kDa). The N-terminal amino acid sequence of the protein bands correspond- ing to the higher -molecular- mass proteins ... Psylinakis E, Bouriotis V & Kokkinidis M (2007) Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus. Functional insights from structural data. FEBS...

Ngày tải lên: 15/03/2014, 11:20

... gambiae thioredoxin reductase, Gly–Cys–selenocysteine–GlyOH in human thio- redoxin reductase, and Cys–X–X–X–X–Cys–GlyOH in the P. falciparum enzyme. These differences offer an interesting approach ... structure of the C-terminal redox cen- tre, reflected in the varying resistance of catalytic inter- mediates to autoxidation. The relevant sequences of this centre are Thr–Cys–Cys–SerOH...

Ngày tải lên: 31/03/2014, 07:20

... wild type + – + – + – + + + ++ + + Mutants Tt4 (pilM::kat )– +–+ –+ ++++++ Tt5 (pilN::kat) +– – –+ ++++++ Tt6 (pilO::kat) +–+ – +++++++ Tt7 (pilW::kat) +–+ – +– –+ – +– Tt8 (pilQ::kat) +–+ – +–+ – +– Tt20 (pilA4::kat) +–+ – +–+ +++ +– AB CD Fig. ... temperature. The secretin-like PilQ was detected in sufficient amounts in the inne...

Ngày tải lên: 07/03/2014, 12:20

... & Weinstein D (1976) Assay of proteins in the presence of interfering materials. Anal Biochem 70, 24 1–2 50. 47 Klingenberg M (1985) End-point UV-methods. In Meth- ods of Enzymatic Analysis ... (PLS) regressions For future metabolic engineering of strains with the purpose of obtaining increased NADPH levels and for the understanding of the regulation of the NADPH l...

Ngày tải lên: 07/03/2014, 17:20

... problem of parse tree features – For instance, in one of the 5- 60 fold data, 34% of syntactic paths in test instances are unseen in training data. For sentences with the average length of more ... the way using auto-parser for Chinese function label- ing is not the optimal choice. 4.5 Error Analysis In the course of our experiment, we wanted to at- tain some under...

Ngày tải lên: 08/03/2014, 01:20

... equili- brated with buffer A (0.1% TFA), and then eluted with a two-step gradient of 0–3 0% buffer B (acetonitrile in 0.1% TFA) in 5–2 0 min and 3 0–1 00% buffer B in 2 0–2 5 min at Recombinant peptides of mung ... Chi 1,2 1 Institute of Protein Research, Tongji University, Shanghai, China 2 Institute of Biochemistry and Cell Biology, Chinese Academy of Science...

Ngày tải lên: 15/03/2014, 09:20