Báo cáo khoa học: The bioactive dipeptide anserine is transported by human proton-coupled peptide transporters doc

... The bioactive dipeptide anserine is transported by human proton-coupled peptide transporters Stefanie Geissler 1 , Madlen Zwarg 1 , Ilka Knu ¨ tter 1 , ... at the intestinal epithelium. The purpose of this study was to investigate whether anserine is a substrate of the H + ⁄ peptide cotransporters 1 and 2 (PEPT1 and PEPT2). We first assessed the ... revised...

Ngày tải lên: 15/03/2014, 09:20

... uncharged state or that the positive charge is no longer proximal to the cosubstrate side-chain. This result is consistent with loss of activity in this mutant when 2-OG is used as the cosubstrate. Higher ... uncoupling of the two reactions, i.e. the stoichiometry is less than 1 : 1. Comparison of the results for assays 1a and 2a imply that the level of 2-OG conversion...

Ngày tải lên: 20/02/2014, 23:20

... to develop their agonists and antagonists. In the present study, we report the identification of the first known molecule antagonizing the activity of the plant hormone cytokinin at the receptor level. ... proteins [17]. The results obtained in the present study further support the hypothesis that the receptors have different structural requirements for the stereochemical...

Ngày tải lên: 16/03/2014, 04:20

... are (labeled) rooted MTs where children are distin- guished by their position. Let s and t be two MTs; t contains s (written as s ~ t) if there exists an in- jective embedding such that (1) ... T(DB, g). The set of all database trees that con- tain every query treegram. (3) Compute the result set R = {t E Cand~(q)l q ! t}. The costly operation in this approach is the las...

Ngày tải lên: 17/03/2014, 23:20

... the His- tag, the mixture was loaded again on the Ni 2+ –Sepharose column. Owing to the moderate intrinsic affinity of PfFNR for the nickel ion, the enzyme was retained by the column even in the ... apicoplast- localised electron transfer pathway involved in the isoprenoid biosynthesis of Plasmodium falciparum. FEBS Lett 579, 6433–6438. 8 Wiesner J & Jomaa H (2007) Isop...

Ngày tải lên: 23/03/2014, 05:22

... to mean that the His-tag is translocated to the trans side of the membrane and then blocks the channel when negative voltages are applied. This is likely, because the His-tag with the linker possesses ... translocate the His-tag at the N-terminal region across the lipid membrane. The same occurs in the case of EqtII; how- ever, the pore formation efficiency of His 6...

Ngày tải lên: 23/03/2014, 10:20

... 5B). Human hepatocyte specific delivery of EGFP by infection The preS1 peptide displayed on the surface of L parti- cles recognizes the specific receptor present on human hepatocytes and is essential ... of the S region is rich in hydrophobic residues. Therefore, the computer-assisted prediction assumes that there should be two a helices, which traverse the ER membrane t...

Ngày tải lên: 23/03/2014, 15:20

... evidence exists on the role of this protein in the biogenesis of heme-containing proteins in plants. To gain insight into this process, we decided to study the role of frataxin using the enzyme ... especially in photosynthetic organisms. Iron as a cofactor is involved in many cel- lular processes: (a) biogenesis of Fe–S proteins accom- plished by the Fe–S cluster machinery locat...

Ngày tải lên: 28/03/2014, 23:20

... prevent hemostasis in the host during hematophagy. In this article, we describe the molecular mechanisms underlying the ability of the leech product saratin to inhibit platelet binding to collagen. In the ... with the process of hemostasis has allowed the leech to continue its alimentary habit of hemato- phagy. The presence of anticoagulants in the salivary glands of the l...

Ngày tải lên: 30/03/2014, 09:20

... contribution to the theoretical P 1/2 shift, suggesting that the DV change, caused by the sucrose or glycerol was associated with the protein stabilization. The origin of the DV change is discussed. The ... these agents, is the same stabilization mechanism involved when the denaturation is induced either by chemical denaturants or by hydrostatic pressure? To answ...

Ngày tải lên: 31/03/2014, 01:20