Báo cáo khoa học: The sigma factors of Mycobacterium tuberculosis: regulation of the regulators potx

... levels. Deletion of the upstream sequence of )507bpcausedthe activity of the promoter to be higher than the activity of pCMV-Luc. However, progressive deletion of the NF-jB binding sites from the CMV ... translocation of NF-jB to the nucleus, where NF-jB facilitates the transcriptional upregulation of genes downstream from the jB motif. The exposure of macrop...

Ngày tải lên: 30/03/2014, 13:20

... [31]. The substrate specificity of Rv0386 may be deter- mined by the concentrations of available ATP and GTP at the cellular location of the enzyme, yet the intracellular concentrations of ATP ... of these proteins. We are aware, of course, that this does not exclude the exist- ence of novel, hitherto unknown cGMP binding pro- teins in the pathogen. In conclusion t...

Ngày tải lên: 07/03/2014, 21:20

... this event, the protein would have a predicted molecular mass of 72 kDa rather than the 75.2 kDa of the pre- dicted product of the full-length ORF. The smaller value is more consistent with the observed ... Louis, MO). Assay of trehalase activity The enzymatic activity of the trehalase was measured by following the release of glucose, either by the reducing sug...

Ngày tải lên: 16/03/2014, 11:20

... replication in the lung during the short-term infection of mice [109,110], the negative regulation of their synthesis by r M negates the possibility of its role in virulence during the early course of infection ... evidence of the interplay of r factors and their interaction with regula- tors, how exactly these factors coordinate with one another in the face o...

Ngày tải lên: 15/03/2014, 09:20

... mice [28]. The instability of DA at the neutral pH of the cytosol causes the forma- tion of toxic aminochromes, and these species are not formed when DA is protonated at the acidic pH of the storage ... counter. Values are the mean of six replicates. The specific uptake was determined by subtracting the uptake counts in the absence of inhibitor from the uptake...

Ngày tải lên: 14/02/2014, 21:20

... observation). In the ternary complex, the histone H3 peptide binds in a deep chan- nel that divides a pair of acidic lobes, one of which is composed of residues from the SET-I region and the other of residues ... present. This is because the con- struct used to determine the structure of the MLL1 SET domain lacks the evolutionarily conserved Win motif in the region...

Ngày tải lên: 16/02/2014, 14:20

... in all of these conditions [22]. In the trau- matically injured brain, however, the synthesis of MT1 and MT2, as well as the synthesis of GIF, are up-regu- lated by reactive astrocytes in the vicinity ... fine processes of these cells. Astrocytic synthesis of GIF is mainly found in the cortex, brainstem and spinal cord [6]. Additionally however, there are also consistent re...

Ngày tải lên: 16/02/2014, 15:20

... [6] and inhibits the tyrosine kinase activity of the IR [7]. On the basis of the crystal structure of the tyrosine kinase domain of the IR in complex with the PIR ⁄ BPS domain of Grb14, Depetris ... we cannot assess whether the kinase activity of the IRs before KCl treatment repre- sents residual activity of receptors with Grb14 bound to them, the activity o...

Ngày tải lên: 18/02/2014, 18:20

... described in the Materials and methods. Completion of the hydrolysis was confirmed by the disappearance of the (Met1)-ONC (M23L) signal in the MALDI-TOF mass spectra 1 h after the addition of AAP. The ... calculating the slope of the curves of Pyr-ONC (M23L) vs. time. From the rates of cyclizationshowninTable1,itcanbeinferredthatthe formation of the N-termin...

Ngày tải lên: 19/02/2014, 12:20

... human cells may affect the metabolism of the target cells in more ways than just the therapeutic purpose of the introduced gene. The normal function of nucleoside kinases is to provide the cells with ... performed according to the instructions of the manufacturer of the membrane (Amersham). The washed membrane was ana- lyzed by PhosphorImager. Incorporation of...

Ngày tải lên: 20/02/2014, 01:20