Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt

... FEBS Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron- encoded protein contribute to its maturase and intron DNA-insertion function Marı ´ a D. Molina-Sa ´ nchez, ... that the C-tail of the RmInt1 intron- encoded protein contributes to the maturase function of this reverse transcri...

Ngày tải lên: 15/03/2014, 09:20

... 1209. Structure of the core region of the Hep4-glycoforms. In the 1 H NMR spectrum of OS-1¢-B (Fig. 5C), anomeric resonances of the three heptose residues (HepI–HepIII) in the inner-core region were ... acetylation at O-4 of GlcI and at O-3 of HepIII, as observed for the Hep3-glycoforms (see above). From the combined data, the structure in Scheme 4 is p...

Ngày tải lên: 08/03/2014, 08:20

... FEBS 3759 Introduction C-peptide is derived from most of the proinsulin seg- ment in between the B and A chains of insulin [1] and has an important structural role in the proper folding and disulfide ... in the presence of trifluoroethanol [15]. In addition, molecular dynam- ics simulations propose turn-like motifs in the mid- region and in the C-termi...

Ngày tải lên: 18/02/2014, 04:20

... splicing. Insertion of a new exon originating from the non-coding sequences of intron 4 of the SAF-1/MAZ gene resulted in creation of a different C-terminus consisting of eight zinc finger domains ... binding of the N-terminus by ancillary factors, (c) the lack of a negatively functioning module that is present in the N-terminus of SAF-1 [26], or (d) all of t...

Ngày tải lên: 07/03/2014, 02:20

... plasmids containing the PHO5 coding region transcribed under the control of the GAL1 promoter, but differing in the length of their 3¢-UTR. To increase the length of the 3¢-UTR we inserted Escherichia ... presence of sublethal amounts of MPA. (B) mRNA levels of the transcription units and strains ana- lyzed in (A) cultured in the absence of MPA. The...

Ngày tải lên: 07/03/2014, 12:20

... of the AP dimer showing the spin- labeled sites. The spin-labeled sites comprised S53, C67, S78, S80, S337 and S373. The other subunit binds to the left of the subunit shown and in front of the ... The S80 position allows the nitroxide spin-label placed there to point into the active site close to the S65 and in the direction of the metal ions...

Ngày tải lên: 16/03/2014, 01:20

... B. The isoalloxa- zine ring of FAD is in yellow. The axis of the pink helix points directly at the C 2 -O of the isoalloxazine. Cys397, covalently bound to the 8a-carbon of the isoalloxazine ring, ... differences within one type of binding fold. With regard to the latter, in most VAO-type covalent flavoproteins, the flavin is covalently linked to an amino a...

Ngày tải lên: 16/03/2014, 01:20

... study was to investigate the intracellular fate of PrP C by examining for the first time its biosynthesis in the secretory pathway of neuro- endocrine cells in vivo and the effect of the transgene expression ... suggesting that the expression of the GFP–PrP C fusion protein in the inter- mediate pituitary is coregulated with these proteins. The POMC and...

Ngày tải lên: 16/03/2014, 14:20

... reported the binding of E2F4 and p130 to the CDE element in G0 [22]. Binding of LIN54 to the adjacent CHR element could therefore stabilize the binding of E2F4 ⁄ p130 at CDE. To address whether the ... important for the binding of LIN54 to the cdc2 promoter (Fig. 5C). We next asked whether the same element was involved in the binding of a LIN54-contai...

Ngày tải lên: 23/03/2014, 04:20

... N-domain, homologous to the N-domains of ClpA or ClpB, and a linker domain homologous to, but half the size of, the linker domain of ClpB [19]. The N-terminal region contains two 32-amino acid ... Clp family of proteins in survival and virulence, it is of interest to understand the mode of action of these proteins in M. tuberculosis. In this study, we f...

Ngày tải lên: 23/03/2014, 06:20