Báo cáo khoa học: microRNA-203 suppresses bladder cancer development by repressing bcl-w expression potx

... 4 shows that bcl-w expression was increased in bladder cancer tissues that showed significantly decreased miR- 203 expression. miR-203 suppresses bladder cancer progression by repressing bcl-w Bcl-w ... miR-203 levels were decreased in bladder cancer samples. bcl-w expression in bladder cancer samples was analyzed by western bloting. The bcl-w levels in b...

Ngày tải lên: 15/03/2014, 00:20

... death induced by rotenone, MPP + , or paraquat. Proc Natl Acad Sci USA 105, 15136–15141. 14 Leong SL, Cappai R, Barnham KJ & Pham CL (2009) Modulation of alpha-synuclein aggregation by dopamine: ... Vime PJ, Gao L, Toledo-Aral JJ, Echevarrı ´ aM&Lo ´ pez-Barneo J (2006) Neuroprotection by transgenic expression of glucose-6-phosphate dehydrogenase in dopaminergic nigrostriatal...

Ngày tải lên: 14/02/2014, 19:20

... role [64]. Interestingly, EspB was shown to be essential for the development of attaching and effacing (A⁄ E) lesions by EHEC by recruiting a-catenin, a cytoskeletal pro- tein that recognizes ... type III secretion activ- ity by Yersinia pseudotuberculosis. J Biol Chem 285, 25269–25284. 61 Tengel T, Sethson I & Francis MS (2002) Conforma- tional analysis by CD and NMR spectrosco...

Ngày tải lên: 14/02/2014, 22:20

... cleavage were observed. TZD-induced adipocyte apoptosis could be blocked by the caspase-3 inhibitor Ac-DEVD-CHO or by overexpression of Bcl2. In cultured rat primary adipocytes, similar apoptosis-inducing ... adipocyte apop- tosis, the expression of PPARc is increased by 80% [10]. Furthermore, the activation of PPARc stimulates apoptosis in a variety of cell types, including cancer...

Ngày tải lên: 16/02/2014, 09:20

... of binding only if the heterodimer is tethered by the wild-type partner. By contrast, we found that, if the heterodimer is tethered to the resin by the mutant partner, the amount of binding observed ... : 250) followed by a secondary rabbit anti-(mouse IgG). The resulting antibody complex was developed with [ 125 I]protein A and detected by means of autoradiography followed by Pho...

Ngày tải lên: 18/02/2014, 08:20

... the manu- script. The work was supported by Slovenian Research Agency (grant P4-0127 J.K. and grant P1-0140 V.T.) and partially by the Sixth EU project Cancerdegra- dome (J.K.). Regulation of ... antibody products: challenges and opportunities. Cancer Metastasis Rev 24, 569–584. 28 Kimby E (2005) Tolerability and safety of rituximab (MabThera). Cancer Treat Rev 31, 456–473. B. Mirkovi...

Ngày tải lên: 18/02/2014, 11:20

... were infected at a multiplicity of infection of 10. Infection was followed by a 72 h expression period. Cells were harvested by centrifugation (1200 g, 10 min), and resuspended in five pellet volumes ... triacylglycerols. HSL activity is controlled by phosphorylation of at least four serines. In rat HSL, Ser563, Ser659 and Ser660 are phosphorylated by protein kinase A (PKA) in vitro...

Ngày tải lên: 18/02/2014, 11:20

... network might exist, we tested for the expression of PDZ scaffolding proteins in the olfactory epithelium using RT-PCR (Fig. 1A). We detected robust expression of MUPP1 and weak expression of ZO-1, but could ... (1 + 2, A C D B Fig. 1. MUPP1 expression in olfactory sen- sory neurons. (A) Expression of mRNA of different PDZ scaffolding proteins in the olfactory epithelium by RT-PCR...

Ngày tải lên: 18/02/2014, 13:20

... of a central b-sheet surrounded by a-helices; the domains are joined by a hinge region contain- ing three segments. c-Cyclodextrin is located at a cleft formed by the two domains. A common functional ... surrounded by a-helices. The two domains are joined by a hinge region, which contains three segments (residues 128– 130, 280–282 and 331–333). The sugar-binding site is located at a...

Ngày tải lên: 19/02/2014, 00:20

... quantification of iNOS mRNA was performed by real-time RT-PCR. The relative level was calculated as the ratio of inflammatory mRNA expression to b-actin mRNA expression. ***P<0.001 versus C; ### P<0.0001 ... l-[ 3 H]citrul- lineÆmin )1 Æmg protein )1 . Real-time quantitative RT-PCR assay of iNOS mRNA expression Quantification of the iNOS mRNA levels was done by SYBR green two-step...

Ngày tải lên: 19/02/2014, 00:20