Báo cáo khoa học: Catalytic mechanism of the primary human prostaglandin F2asynthase, aldo-keto reductase 1B1 – prostaglandin D2 synthase activity in the absence of NADP(H) pptx
Báo cáo khoa học: Catalytic mechanism of the primary human prostaglandin F2asynthase, aldo-keto reductase 1B1 – prostaglandin D2 synthase activity in the absence of NADP(H) pptx
... compilation ª 2011 FEBS Catalytic mechanism of the primary human prostaglandin F 2a synthase, aldo-keto reductase 1B1 – prostaglandin D 2 synthase activity in the absence of NADP(H) Nanae Nagata 1 , ... as the (a ⁄ b) 8 -barrel fold [ 3–6 ]. The nucleotide cofactor binds in an extended conformation at the top of the a ⁄ b-barrel, with th...
Ngày tải lên: 14/03/2014, 23:20
Tài liệu Báo cáo khoa học: Catalytic mechanism of SGAP, a double-zinc aminopeptidase from Streptomyces griseus pdf
... exact binding mode of the hydroxide to the metal ions, the proton pathway in catalysis and the specific involvement of the catalytic residues in the enzymatic reaction. The two zinc ions in the active ... protein residues near the active site, whereas in AAP the free amine interacts with the second zinc ion (Zn2) [2 4–2 8]. Open issues regarding the cat...
Ngày tải lên: 19/02/2014, 02:20
Báo cáo khoa học: Targeting mechanism of the retinoblastoma tumor suppressor by a prototypical viral oncoprotein Structural modularity, intrinsic disorder and phosphorylation of human papillomavirus E7 doc
... equilibrium, increasing the PII propen- sity of the E7 CR2 region. The E7 C-terminal domain binds independently to RbAB The increased affinity of the full-length E7 protein compared with the E7 N-terminal ... specificity of the E7C–RbAB interac- tion. A peptide containing the CR2 region of E7 did not compete with E7C binding, indicating that E7C does not bind to the Rb...
Ngày tải lên: 22/03/2014, 21:20
Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc
... present in the spleen of these mice. For the determination of the number of PMM56 bacteria present in the spleen of these four mice, we thus chose a value of 50 CFU per spleen. The average number of ... determined by calculating the ratio of mutant to wild-type bacteria after correcting for the ratio of these strains in the inoculum. It appeared that, 21...
Ngày tải lên: 23/03/2014, 09:20
Báo cáo khoa học: Spectroscopic investigation of the reaction mechanism of CopB-B, the catalytic fragment from an archaeal thermophilic ATP-driven heavy metal transporter potx
... significant shifts of the a, b and c absorbance bands, resulting from strong interactions of phosphate groups with amino acid side chains lining the nucleotide binding site of the protein [26,3 1–3 4]. It ... production of inorganic phosphate was observed in the absence of Mg 2+ , which indicates that Mg-ATP is the substrate of CopB-B. Furthermore, the ATPase act...
Ngày tải lên: 07/03/2014, 00:20
Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... excluded all residues involved in the binding of the metal cofactor. ApeSODs in different forms were superimposed with the least square fit of Ca atoms of the residues in the range 1 0–2 00. ApeSOD and ... metal-binding site at the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
... named according to the residue in the mid- point of the respective loop, as shown in Fig. 2. To the east of the active site the 37- and 60-loops border the S2¢ pocket of the proteinase. The observed ... formation of the catalytic machinery and structures required for binding of the main chain of the substrate peptide and proper positioning of the...
Ngày tải lên: 19/02/2014, 00:20
Báo cáo khoa học: Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafficking of human acetylcholinesterase and butyrylcholinesterase potx
... counterparts, in agreement with the higher mass of BChE subunits [37]. This indicates a predominant in uence of the catalytic domain on oligomerization. Role of the C-terminal cysteine Mutation of the ... modified, suggesting that degradation was increased by suppres- sion of the cysteine. Thus, it appears that the effect of a C-terminal cysteine on the traffickin...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt
... Subsequently, the GST–PP2Aca:His–TIPRL complex was incubated with His–a4 and the GST–PP2Aca:His–a4 complex was incubated with His–TIPRL. Binding of His–TIPRL to the previously formed GST–PP2Aca:His–a4 ... occlusion of the active site, in vitro binding assays were performed in the presence of the PP2Ac inhibi- tor okadaic acid. These assays showed that binding of His–TI...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldfish pot
... in inhibitor binding and suggest the proteins use a similar catalytic mechanism. These findings underline the importance of the evolutionarily con- served phosphodiesterase activity of 2H proteins ... regrowth upon the optic nerve crush, and also expressed in the germinal neuroepithelium of retina, which generates new neurons throughout the lifespan of the fish [1...
Ngày tải lên: 07/03/2014, 10:20