Báo cáo khoa học: Re-evaluation of the function of the F420dehydrogenase in electron transport ofMethanosarcina mazei potx

... absent in the cyto-plasmic fraction of the DfpoF mutant.This observation prompted investigation into the function of FpoF in more detail. The correspondinggene, mm0627, was expressed in E. ... in favour of this hypothesiscame from the ﬁnding that the rate of F420H2-depen-dent heterodisulﬁde reduction in cell lysates of the DfpoA-O mutant or the DfpoF mutant was in the range of ... present in Ms. mazei. Instead, the gene product FpoF is responsible for F420H2oxidation and functions as the electron- input device[3]. Interestingly, the gene fpoF is not part of the operon...

Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt

... contained a 30 bp insertion in the ORF, beginning at nucleotide 516. This resulted in insertion of a 10 amino acid segment (TREIDYPETI)and one substitution (Ser ﬁ Gly) at the C-terminalend of the ... ortho-log of the product of this gene. In the clade includingSpa¨tzle-1, the branch lengths are noticeably longer and the bootstrap values are lower than in the other cladescontaining Spa¨tzle-2 ... to obtain the missing ends of the cDNA, and then used primers encompassing the start and stop codons, with larval fat body cDNA astemplate, to obtain eight individual clones containing the complete...

Báo cáo khóa học: Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha pptx

... The protein part that holds two of the Fe-S clusters in the small subunit of the D. gigas enzyme is missing in HoxY of the SH, suggesting anexposure of part of the hydrophobic surface of the HoxH ... changes of the Ni-Fe site. The Fe-S clusters in the HoxFUY subunitsand the ﬂavin in the HoxF subunit are all considered tobe functional in the intramolecular electron transferduring the H2-NAD+reaction.It ... was 25%higher than the original activity (16.8 UÆmg)1), indicatingthat part of the original enzyme molecules did not containFMN-a.Integrity of the SH during the release of FMN-aOur experiments...

Tài liệu Báo cáo khoa học: a-Conotoxins as tools for the elucidation of structure and function of neuronal nicotinic acetylcholine receptor subtypes doc

... argues against a role for a3intheformation of native MII binding sites, but may reﬂect the scarcity of these sites in the investigated brain tissues and/or the formation of low afﬁnity binding sites ... microdomains within the same bindingsite [35]. The proline residue in position 6, which isconserved in all other a-conotoxins, appears to be the major determinant of the abilities of ImI and ... studies. The highselectivity of the a-conotoxins, together with the possibility of obtaining detailed information on their three dimen-sional structures and the relative ease of synthesis, makesthem...

Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt

... tripalmitin as standards. The scatteringpatterns were collected in 15 frames of 1 min to verify the absence of radiation damage. The experimental datawas normalized to the intensity of the incident ... enol form of indolepyruvate (Fig. 1). In the course of the establishment of the equilibrium % 85% of the enol form is converted into the ketone (half-time% 8 min at 20 °C) as deduced from the appearance ... withidentical integrals of all signals). The additional singlet of the pyruvyl moiety at 6.65 p.p.m. with a relative integral of 1 with respect to the indole protons is consistent with the occurrence of the...

Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

... successful metabolic engineering of freezing tolerance in plants should include such ananalysis of the dynamics of metabolism to gain com-prehensive information about the effects of individualoverexpression ... simulta-nous reduction in hexose concentration, particularlyduring the initial period of cold exposure. Using RNAinterference-mediated inhibition of the dominating vac-uolar invertase ATbFRUCT4 ... integral of carbon uptake perhour. Data points were spline-interpolated to obtain time-continuous information on net photosynthesis during the whole period of cold exposure. The rate of rafﬁnose...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... N7 (Fig. 1C). The 2¢OH of the ribose is hydro-gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the riboseare involved in coordinating a putative ... solutions. The ligandbound was interpreted as GMP because the N2 of the base makes a hydrogen bond to a main chain car-bonyl. Binding of a xanthine base from XMP in the same position, would lead to the ... for the quantitation of microgram quantities of proteinutilizing the principle of protein-dye binding. AnalBiochem 72, 248–254.28 Page R & Stevens RC (2004) Crystallization data min-ing in...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... all-alpha domain, STATprotein DNA-binding domain and SH2 domain of stat6, and the zinc-ﬁnger domain, leucine-zipperdomain and fork-head domain of foxp3. In addition,for stat6 and foxp3, there were ... adjacent DNA-binding sites,a coiled-coil STAT protein all-alpha domain, which isimplicated in other protein–protein interactions, aSTAT protein DNA-binding domain and an SH2domain, which binds phosphorylated ... areas of amino acid conservation throughout the vertebrates.Signiﬁcant homology was seen in the putative T-boxDNA-binding domain of t-bet, the STAT protein inter-action domain, STAT protein all-alpha...

Tài liệu Báo cáo khoa học: Post-translational modiﬁcation of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx

... strains [3]. PathogenicYersiniae are typically taken up through ingestion andﬁrst reach the intestine. The Yersinia surface proteininvasin binds to b1 integrins on the apical surface of M ... In addition to actin, other host cell proteins have beenshown to bind to the virulence factor YpkA, includingOTUB1 [13]. In order to investigate the role of OTUB1 in Yersinia invasion of HEK293T cells, ... serine ⁄ threonine kinasethat phosphorylates actin [12], binds the deubiquitinat-ing enzyme otubain 1 (OTUB1) [13,14], the smallG protein subunit Gaq [15] and interacts with mem-bers of the...

Tài liệu Báo cáo khoa học: Post-translational modiﬁcations of the linker histone variants and their association with cell mechanisms docx

... number of these modiﬁcations is low, the density of modiﬁcations is much the same as in the rest of the lin-ker histones. It is the shortness of the N-terminal tailsTable 1. Alignment of chicken, ... the triphosphate form being incorpo-rated into DNA in place of the natural base cytosine[12]. The use of such analogues results in the globaldepletion of DNMTs and a subsequent reduction in DNA ... in Swiss-Prot and similar databases.Structure and function of the linkerhistone variantsLocation and structure of the linker histonesHistorically, the location of the globular domain of the linker...

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