Báo cáo khoa học: Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris ppt

... cell, indicating that it belongs to the class of nucleocytoplasmic jacalin-related lectins. Since the expression level of Orysata in rice tissues is very low the lectin was expressed in the methylotrophic ... simi- larly docked into the saccharide-binding site of Calsepa (RCSB Protein Data Bank code 1OUW) [28]. Cartoons showing the docking of Man ⁄ MeMan in...

Ngày tải lên: 14/03/2014, 23:20

... FEBS 4773 of Q A – in PSII centers that are affected by the nonsatu- rating concentration of inhibitor [7,8]. The E m of Q A is raised in the presence of DCMU in the Q B pocket, making it energetically ... N,N,N¢,N¢-tetramethyl- p-phenylenediamine [23,24]. The use of a nonsaturating concentration of DCMU, an inhibitor known to close the PSII reaction center by bi...

Ngày tải lên: 19/02/2014, 05:20

... 1A,C). These findings indicate that the region between amino acids 28 and 139 of DEC1 (including the bHLH domain) is sufficient for the suppressive activity of DEC1. The C-terminal region (amino acids ... region of DEC1, including the bHLH domain, interacted with the C-terminal r egion o f BMAL1 in a mammalian two-hybrid assay. Accordingly, a Fig. 5. Analysis of DEC1 mut...

Ngày tải lên: 19/02/2014, 16:20

... structure of the N-terminal domain of the human homologue, solved at 2.2 A ˚ resolution [11]. The obtained model covers residues 254–568 of HpPex5p, corresponding to the C-terminal half of the protein ... concluded from the structural model of the PTS1-binding C-terminal domain of HpPex5p (Fig. 4). In this domain three of the seven Trp residues of HpPex5p a...

Ngày tải lên: 21/02/2014, 00:20

... flavin-binding domain of ferre- doxin : NADP + reductase [10]. The lysine residue is not conserved; instead, an arginine residue interacts with the FAD molecule of the enzyme. In the case of FMN-binding ... Blanche F (1995) Purification of the two-enzyme sys- tem catalyzing the oxidation of the d-proline residue of pristinamycin IIB during the last step of pristi...

Ngày tải lên: 07/03/2014, 02:20

... (two cDNAs). Initial mapping of the region of PP2Ac involved in TIPRL binding was obtained from the cDNAs that showed positive interaction with TIPRL. The extension of these cDNAs is shown in Fig. ... with GST–a4 in the presence of His–PP2Aca (data not shown). Similar results were obtained using the PP2Ac-binding domain of a4, a4D222 [24], instead of the full-l...

Ngày tải lên: 07/03/2014, 05:20

... anal- ysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the two modes of aglycone binding. The amino acid sequence of Zm-p60.1 was com- pared with the ... distances of reacting atoms. The only noted difference was in the orienta- tion of the aromatic ring of pNPGlc in the F193A mutant (Fig. 6D), owing to...

Ngày tải lên: 16/03/2014, 04:20

... [5]. Furthermore, a chromosomal point mutatio n in srfA-B, changing a D to A in the His-motif (italic) of the corresponding Asp domain of the surfactin synthetase was found to abolish surfactin production ... bound in the binding poc ket o f a nother e nzyme molecule within the active homomultimer. This could explain the distance in sequence homology with NRPSs C-domai...

Ngày tải lên: 17/03/2014, 11:20

... excluded all residues involved in the binding of the metal cofactor. ApeSODs in different forms were superimposed with the least square fit of Ca atoms of the residues in the range 10–200. ApeSOD ... focus on the coordination of the metal cofactor in the active site as well as the changes it experiences in response to different metal cofactors. Finally, by c...

Ngày tải lên: 14/02/2014, 22:20

... 5-aza-2¢-deoxycytidine, resulted in the redistribution of CENP-B [24], indica- ting that the CpG methylation affects the CENP- B–DNA interaction. Our structural analysis of the CENP-B DNA-binding domain [CENP-B(1–129)] complexed ... sharply kinked by the CENP-B binding are encircled in red. The nucleotide sequences of sites 1 and 3 are indicated in the left column....

Ngày tải lên: 16/03/2014, 18:20