Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx

... The Authors Journal compilation ª 2011 FEBS 2043 Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding ... free cysteines. In conclusion, we show a mechanism of action of bacitracin on PDI that involves covalent binding of an open thiol form of bac...

Ngày tải lên: 14/03/2014, 23:20

... sys- tems showed the end of the melting process at 41 °C. The decrease of the T m in PA-containing vesicles is explained by the partial extraction of PA from the bilay- er by the formation of PA ⁄ Ca 2+ complexes. ... adsorb onto the platinum wire. An important point in this step is to avoid dehydration of the sample to maintain the integrity of the...

Ngày tải lên: 30/03/2014, 11:20

... of b-turn in CD2 protein. In addition, the binding sites of the peptides to CD58 protein predicted by autodock were near the CD2–CD58 interface, either in the topcavityofCD58orneartheCC¢ sheet ... more potential since it may directly interrupt the binding mech- anism of CD2–CD58. Inhibition of cell adhesion involves interrupting the interaction of key residues...

Ngày tải lên: 19/02/2014, 13:20

... and the recombinant protein. All sera reacting with the natural protein in the ELISA were positive in the immunoblotting experiments. For the recombinant protein, 24 of 28 sera were negative in both ... release of the basophils, the allergen- induced histamine release was calculated as percent of the total amount of histamine determined after lysis of th...

Ngày tải lên: 21/02/2014, 00:20

... density of the headgroup of the latter, leading to different migration in the HPLC purification process. Additionally, the absence of a lipoprotein is confirmed by the absence of TLR2 reactivity in the ... preincubated with unlabeled LPS and recombinant human lipopolysaccharide-binding protein LBP was added. Endotoxin activity determination by the chromogenic...

Ngày tải lên: 21/02/2014, 00:20

... in the substrate-binding domain and strand E2 in the hinge domain, two residues appear to act, at least partly, as structural determinants for the closed conformation of the substrate loop. The ... positioning of the aromatic ring of Tyr456 in the closed state. In the absence of a closed complex of E542K P2Ox, the effect of the loss of this hydro...

Ngày tải lên: 07/03/2014, 03:20

... the x-intercept of the RNase activity plot. The oxidase activity matches the slope of the linear plot of reciprocal of lag times against the PDI concentrations in units of lm )1 Æmin )1 . The Z ... For the rate of decrease of the linear form, the original data were fitted by Y(t) ¼ e –kt · 100%, where Y is the percentage of the linear form, and t...

Ngày tải lên: 07/03/2014, 05:20

... compared with the control groups. In addition, we constructed another EGFP reporter vec- tor containing mutations in the regions encoding the miR-373 binding sites (Fig. 4A). Neither blocking of miR-373 ... of miR-373 with ASOs nor overexpression of miR-373 had any effect on the intensity of EGFP fluorescence from the vector containing mutations in the region enco...

Ngày tải lên: 14/03/2014, 23:20

... uptake into liposomes as a result of the decrease in pH was assayed by measuring the radioactivity of tritium, as described in Materials and methods (In the figure, the y-axis represents the cpm ... When the proteoliposomes were suspended in the indicated acidic buffer solutions containing [ 3 H], the radioactivity inside the proteolipo- somes increased with de...

Ngày tải lên: 29/03/2014, 23:20

... insecticidal activity of these proteins has been evaluated, it is unclear whether the toxins in the regurgitant of antlions are limited to these two proteins, and whether other symbionts of the larvae ... obtained by tryptic digestion of the protein. Cloning and sequencing of the insecticidal toxin ( SMC ) gene of B. cereus The SMC-encoding gene was amplified b...

Ngày tải lên: 30/03/2014, 13:20