Báo cáo khoa học: N-glycosylation influences the structure and self-association abilities of recombinant nucleolin pot
Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... consti- tute the protein structure. Here, we report the analysis of the contribution of these putative domains to the structure/ function of recombinant human GCPII. We cloned 13 mutants of human ... absence of the intracellular and transmem- brane domains does not influence carboxypeptidase activity of GCPII and neither does the attachment of the His- Xpre...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III ppt
... works 4453 of the S1-bound chloride anion and ‘pushes’ the Cl ) out of the bottom of the S1 pocket (the occupancy of the Cl ) in this structure is 0.7). The alternate confor- mation of the Asp443 ... with the occupancy of the S1 pocket by a ligand and the ‘open–close’ transition exploits the hinges on the two sides of the lid made up of Asn540...
Ngày tải lên: 23/03/2014, 05:22
Báo cáo khoa học: Sulfated polysaccharides inhibit the catabolism and loss of both large and small proteoglycans in explant cultures of tendon doc
... inclusion of these sulfated agents there was significant inhibition (40%) of rate of loss of 35 S-labeled large proteogly- cans from the matrix by the end of the culture period. Similarly, by day 10 of ... that the rate of [ 35 S]sulfate appear- ing in the medium was suppressed by the inclusion of the sulfated polysaccharides. At the end of the culture peri...
Ngày tải lên: 23/03/2014, 10:21
Báo cáo khoa học: "Conundrums in Noun Phrase Coreference Resolution: Making Sense of the State-of-the-Art" docx
... B 3 presumes that the gold standard and the system response are clusterings over the same set of CEs. This, of course, is not the case when the system automat- ically identifies the CEs, so the scoring ... coreference elements, and knowledge of all and only the annotated CEs. We also measure the performance of state -of -the- art resolvers on sev- eral classes...
Ngày tải lên: 30/03/2014, 23:20
Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... understanding of the structure of both the Ab monomer and its various assemblies could help in the design of new therapeutic strategies targeted at preventing the formation or ameliorating the activity of ... Subramanian and Shree [42], there was no rigorous assessment of the purity of the product or the correctness of the sequence. In con- trast, the p...
Ngày tải lên: 18/02/2014, 13:20
Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot
... plate- lets and endothelial cells. The important role of these glycans in pharmacokinetics has been demonstrated by the slower and smaller increment of FDP in vivo after the injection of desialylated ... sequenced. Of these, 10 clones were found to encode the LC2 and LC1 sub- units. Others were found to encode other variants of the C-lectin-like venom proteins. The...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt
... purified from the gut of larvae of the yellow-spotted longicorn beetle Psacothea hilaris by acetone precipitation and elution from gels after native PAGE and SDS/PAGE with activity staining. The purified ... cockroaches and crayfish) and GHF 45 (mussel and beetle). These three families are structurally unrelated and their evolutionary origins are likely to be independent. L...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khoa học: Metal ions modulate the folding and stability of the tumor suppressor protein S100A2 docx
... on the thermodynamic stability of the Ca 2+ - and Zn 2+ -loaded mutants can be retrieved from the anal- ysis of metallation effects within the background of the same mutation (Table 2). With the ... to the high-affinity site. These effects of metal ions on S100A2 folding and stability contribute to a better understanding of the Ca 2+ - and Zn 2+ -dependent regul...
Ngày tải lên: 30/03/2014, 02:20
Báo cáo khoa học: Atg8L/Apg8L is the fourth mammalian modifier of mammalian Atg8 conjugation mediated by human Atg4B, Atg7 and Atg3 docx
... tagged protein. Merging of the images indicated that most of the puncta of CFP–Atg8L were colocalized with those of YFP–LC3 (Fig. 4,f,I,l). In the absence of inhibitors, only a few puncta of either type were ... [21]. The amino acid sequence of mouse Atg8L ⁄ Apg8L shows 100% and 54% identity to those of human Atg8L and yeast Atg8, respectively, and 33%, 60% and...
Ngày tải lên: 30/03/2014, 11:20
Báo cáo khoa học: Structural basis for the changed substrate specificity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D docx
... comparing the phosphorylation of the natural substrates of the double mutant with the single mutant, it seems that the mutation N45D in the double mutant counteracts the negative effect(s) of the N64D ... 3737 actions with the 3¢-OH of the deoxyribose of the substrate. The absence of this hydrogen bond and a flexible LID make the substrate binding pocket...
Ngày tải lên: 30/03/2014, 20:20