Báo cáo khoa học: An unusual plant triterpene synthase with predominant a-amyrin-producing activity identified by characterizing oxidosqualene cyclases from Malus · domestica ppt

... Zealand Institute for Plant and Food Research Limited. Journal compilation ª 2011 FEBS 2499 An unusual plant triterpene synthase with predominant a-amyrin-producing activity identified by characterizing oxidosqualene ... and MdOSC3). MdOSC1 and MdOSC2 were expressed by transient expression in Nicotiana benthamiana leaves and by expression in the yeast Pichia met...

Ngày tải lên: 14/03/2014, 23:20

... the quality of the translations supplied by an MT system require manual post-editing. This serial process pre- vents the MT system from integrating the knowledge of the human translator. An alternative ... take advantage of the exist- ing MT technologies is to use them in collaboration with human translators within a computer-assisted translation (CAT) or interactive framework (Isab...

Ngày tải lên: 07/03/2014, 22:20

... manipulating a small, highly abstract grammar is 4Fully iustantiated grammars have been used, among others, by Tomita(1985) in an LR parser, and by Doff(1990), Fong(1991) in GB-based parsers. sit ... algorithms, the size of the graxrtrnsr (which iS a constant factor) can easily become the predominant factor for a11 useful inputs (see Berwick and Weinberg 1982). Work on compil...

Ngày tải lên: 23/03/2014, 20:20

... carried out by using a Shimadzu UV-2550 spectropho- tometer (Kyoto, Japan). b-CD was purchased from Shanghai Sanpu Chemical Plant (Shanghai, China), recrystallized three times from water and dried ... lipid membranes and other cellular components against oxidative damage [1–4]. It is related to many diseases and is regarded as one of the most important antioxidant enzymes in living or...

Ngày tải lên: 19/02/2014, 02:20

... 950–956. 49 Wang Q, Zhang H, Kajino K & Greene MI (1998) BRCA1 binds c-Myc and inhibits its transcriptional and transforming activity in cells. Oncogene 17, 1939–1948. 50 Zhang H, Somasundaram ... supplemented with 10% fetal bovine serum, penicillin (100 unitsÆmL )1 ) and strepto- mycin (50 lgÆmL )1 ). Transient transfection Lipofectamine was obtained from Invitrogen and used for th...

Ngày tải lên: 16/03/2014, 02:20

... purchased from Invitrogen. Horseradish peroxidase linked donkey anti-rabbit IgG and sheep anti-mouse IgG antibodies were obtained from GE Healthcare (Piscataway, NJ, USA). Cell culture and transfection The ... Invitrogen) and transferred onto poly(vinyli- dene difluoride) membranes (Millipore, Billerica, MA, USA) by the appliance of an electric field (100 V for 1 h). The transfer efficie...

Ngày tải lên: 29/03/2014, 21:20

... symptoms of an acute gouty attack and be made aware that changes in certain medication dosages may precipitate an attack. Awareness of radiographic changes associated with gout is still of importance ... out infection. Conclusion This is an uncommon and unusual case of gout in the wrist which occurred in isolation and which may have been induced by a change in anti-hypertensive me...

Ngày tải lên: 25/10/2012, 10:06

... reviewing the manuscript. This study was supported by NIH grants EY018659, EY012231 and EY019309; a grant (P20RR024215) from the National Center for Research Resources; and a grant from OCAST. References 1 ... membrane was then incubated for 1.5 h with a 1 : 25 000 dilution of anti-mouse IgG conjugated with Dy- Light 549 and anti-rabbit IgG conjugated with DyLight 649 (Pierce, Roc...

Ngày tải lên: 14/02/2014, 14:20

... were washed once with NaCl ⁄ P i , and then incubated in medium with 1 · 10 )8 m PA and 1 · 10 )8 m LF for 4 h or 24 h. The cells were resuspended in medium, stained with trypan blue, and counted ... 9500 g for 30 min, and the supernatant was decanted. The pellet was resuspended in 10 mL of supernatant, and then centrifuged at 20 000 g for 30 min. The supernatant was decanted, a...

Ngày tải lên: 16/02/2014, 09:20

... localization of PBX1 and EXD proteins depends on nuclear import and export signals and is modulated by association with PREP1 and HTH. Genes Dev 13, 946–953. C. Hyman-Walsh et al. Meis2 transcriptional ... within these proteins that can mediate both positive and negative influences on transcriptional activity. Interestingly, splice variants of mammalian Meis1 and Meis2, and Drosophila HTH...

Ngày tải lên: 16/02/2014, 15:20