Mucins Methods and Protocols doc

... Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina , Chapel Hill , NC , USA 1 Michael A. McGuckin and David J. Thornton (eds.), Mucins: Methods and Protocols, Methods in Molecular ... Science+Business Media, LLC 2012 Chapter 1 Mucin Methods: Genes Encoding Mucins and Their Genetic Variation with a Focus on Gel-Forming Mucins Karine Rous...

Ngày tải lên: 14/03/2014, 22:20

... 2 and 0.5 m L of linear pCR2.1 TOPO plasmid. 2. The reactions were mixed, and then let stand at room tempera- ture for 40 min ( see Note 6 ). The protocols used for transformation and ... aggrecan cDNA domain structure and aggrecan structure. The drawings indicate the approximate locations of the globular domains (G1, G2 and G3) and the keratan sulfate (KS) and chondr...

Ngày tải lên: 05/03/2014, 23:20

... Toxin Methods and Protocols Edited by Dana Philpott Frank Ebel 8 Karmali Methods for Detection of STEC in Humans 9 9 From: Methods in Molecular Medicine, vol. 73: E. coli: Shiga Toxin Methods and ... Significance of STEC Infections 1 1 From: Methods in Molecular Medicine, vol. 73: E. coli: Shiga Toxin Methods and Protocols Edited by: D. Philpott and F. Ebel © Humana...

Ngày tải lên: 23/03/2014, 07:20

... with 0.1% CaCl 2 at 37°C Histological Methods for Detection of Mucin 29 29 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana ... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin h...

Ngày tải lên: 08/10/2012, 10:25

... Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The Mucins (Corfield, ... K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols...

Ngày tải lên: 08/10/2012, 10:25

... gel-forming mucins in particular, there are few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited ... and identification of the mucins present in mucous secretions or from cell culture, focusing primarily on those mucins involved in gel formation. At present, the mucins M...

Ngày tải lên: 08/10/2012, 10:25