Mucins Methods and Protocols doc

... Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina , Chapel Hill , NC , USA 1 Michael A. McGuckin and David J. Thornton (eds.), Mucins: Methods and Protocols, Methods in Molecular ... Science+Business Media, LLC 2012 Chapter 1 Mucin Methods: Genes Encoding Mucins and Their Genetic Variation with a Focus on Gel-Forming Mucins Karine Rous...

Ngày tải lên: 14/03/2014, 22:20

... 2 and 0.5 m L of linear pCR2.1 TOPO plasmid. 2. The reactions were mixed, and then let stand at room tempera- ture for 40 min ( see Note 6 ). The protocols used for transformation and ... aggrecan cDNA domain structure and aggrecan structure. The drawings indicate the approximate locations of the globular domains (G1, G2 and G3) and the keratan sulfate (KS) and chondr...

Ngày tải lên: 05/03/2014, 23:20

... Toxin Methods and Protocols Edited by Dana Philpott Frank Ebel 8 Karmali Methods for Detection of STEC in Humans 9 9 From: Methods in Molecular Medicine, vol. 73: E. coli: Shiga Toxin Methods and ... Significance of STEC Infections 1 1 From: Methods in Molecular Medicine, vol. 73: E. coli: Shiga Toxin Methods and Protocols Edited by: D. Philpott and F. Ebel © Humana...

Ngày tải lên: 23/03/2014, 07:20

... by Anthony P. Corfield The Mucins Glycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11 Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical ... monitor and collect fractions using a fraction col- lector and subject to the appropriate carbohydrate and antibody analyses. 3.6. Analysis of Mucins Methods for the detec...

Ngày tải lên: 08/10/2012, 10:25

... with 0.1% CaCl 2 at 37°C Histological Methods for Detection of Mucin 29 29 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana ... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin h...

Ngày tải lên: 08/10/2012, 10:25

... Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The Mucins (Corfield, ... K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols...

Ngày tải lên: 08/10/2012, 10:25

... GI Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., Totowa, ... protective barrier and a stable unstirred layer with its own microenvironment, between the mucosal sur- face and the lumen (1,2). In the mouth, where salivary mucins are secreted, and the eso...

Ngày tải lên: 08/10/2012, 10:25

... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana ... Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial mucins are secretory ... Pho...

Ngày tải lên: 08/10/2012, 10:25

... gel-forming mucins in particular, there are few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited ... and identification of the mucins present in mucous secretions or from cell culture, focusing primarily on those mucins involved in gel formation. At present, the mucins M...

Ngày tải lên: 08/10/2012, 10:25